百晓学者学者目录

黄光明

副教授、博导、教授、讲师,中国科学技术大学

精选作品

期刊论文

2026

Integrating Aptasensor With Mass‐Tag Signal Amplification Strategy for the Detection of Three Steroid Hormones in Serum

Jia Zhao, Huimin Liu, Liujuan Zhan, Weizhou Shao, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2026

Mapping Cell Metabolic States by Image-Enabled Gating Metabolomic Cytometry.

Yuanyi He, Zijun Ren, Xiao-Jie Chen, Lecheng Lyu, Zhi-Cheng Liu, Wen-Zhen He, Xiao-Hu Zheng, Guang-Ming Huang

Analytical Chemistry

2026

Single-bacterium metabolome revealing heterogeneous cellular states in bacterial populations

Liujuan Zhan, Huimin Liu, Wen-Jiao Chang, Kai-Ming Cao, Shangpu Zhang, Yiwen Gao, Baoyou Chu, H. Yin, Hong-Bin He, Rong-Bin Zhou, Xiao-Ling Ma, Zhuanghao Hou, Guangming Huang

Nature Communications

2025

诱导纳升电喷雾串联质谱法区分单细胞中柠檬酸/异柠檬酸

零晨,侯壮豪,黄光明

《质谱学报》

2025

Automated Chronological Sequence Analysis of Intersecting Lines between Laser Printing and Seals via Pattern Selection-Based DESI-MS Imaging.

Ying Chen, Huimin Liu, Yiting Yuan, Da Qin, Zhuanghao Hou, Guangming Huang

Analytical Chemistry

2025

Direct Metabolic Profiling of Biofluids via Wimshurst Machine–Nanoelectrospray Ionization Mass Spectrometry

Qiming Kang, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2025

Solid‐Phase Extraction Coupled to Tandem Mass Spectrometry for Rapid Quantitation of Urinary Catecholamines and Total Metanephrines

Yu-Han Li, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2025

Dual Desalting Electrospray Strategy for In-Cell Mass Spectrometry to Reveal Novel Sphingolipid Metabolism in an Epithelial-Mesenchymal Transition.

Huimin Liu, Tianhong Wu, Hong-Bin He, Rong-Bin Zhou, Jia Zhao, Liujuan Zhan, Zhuanghao Hou, Guang-Ming Huang

Analytical Chemistry

2025

Nondestructive Metabolic Monitoring of Living Organisms by Water-Droplet Extraction and Contact-Free Electrospray Ionization Mass Spectrometry.

Yuanyi He, Xiaojie Chen, Lecheng Lyu, Richard N. Zare, Guang-Ming Huang

Analytical Chemistry

2025

Effect of Dimethyl Sulfoxide on Lysozyme Structural Stability During Electrospray Ionization Revealed by Molecular Dynamics Simulations

Zhuoyuan Du, Mou-Jun Luan, Zhuanghao Hou, Guangming Huang

Chemical Research in Chinese Universities

2025

Metal-induced nanoscale clusterization initiates protein liquid-liquid phase separation.

Sijia Xiang, Zhuanghao Hou, Yu Wang, Yang Yang, Hong-Ze Hu, Changbao Yin, Guangming Huang, Kai-Ming Cao, Yang-Zhong Liu

Cell Reports

2025

In-Cell Mass Spectrometry and Ultraviolet Photodissociation Navigates the Intracellular Protein Heterogeneity.

Shirui Yang, Zhuanghao Hou, Zhe-Yi Liu, Zhixiong Jin, He Zhao, Kai-Ming Cao, Shan Zhao, Wei-Qing Zhang, C. Xiao, Xue-Ming Yang, Guang-Ming Huang, Fang-Jun Wang

Journal of the American Chemical Society

2024

Paper Spray Ionization Mass Spectrometry Coupled with Paper-Based Three-Dimensional Tumor Model for Rapid Metabolic Gradient Profiling.

Huimin Liu, Liujuan Zhan, Jia Zhao, Shan Zhang, H. Yin, Zhuanghao Hou, Guang-Ming Huang

Analytical Chemistry

2023

Inter-Domain Repulsion of Dumbbell-Shaped Calmodulin during Electrospray Ionization Revealed by Molecular Dynamics Simulations.

Mou-Jun Luan, Zhuanghao Hou, Buchun Zhang, Likun Ma, Si-Ming Yuan, Yang-Zhong Liu, Guang-Ming Huang

Analytical Chemistry

2023

Recent advances in single bacterium metabolic analysis techniques

Liujuan Zhan, Huimin Liu, Zhuanghao Hou, Yi-Wen Gao, Baoyou Chu, Guang-Ming Huang

Trends in Analytical Chemistry (TrAC)

2023

Metabolite profiling and identification in living cells by coupling stable isotope tracing and induced electrospray mass spectrometry.

Zhuanghao Hou, Liujuan Zhan, Kai-Ming Cao, Mou-Jun Luan, Xinchen Wang, Buchun Zhang, Likun Ma, Hao Yin, Zhi-Cheng Liu, Yang-Zhong Liu, Guang-Ming Huang

Analytica Chimica Acta

2022

Native Mass Spectrometry for Peptide-Metal Interaction in Picoliter Cell Lysate.

Zhuanghao Hou, Mou-Jun Luan, Liujuan Zhan, Xinchen Wang, Si-Ming Yuan, Kai-Ming Cao, Yaping Sheng, Hao Yin, Yang-Zhong Liu, Guang-Ming Huang

Analytical Chemistry

2022

Whole-brain mapping of metabolic alterations in a mouse model of Alzheimer's disease by desorption electrospray ionization mass spectrometry imaging

Xiaoqun Wang, Xiaoli Yang, Zhuanghao Hou, Shuangshuang Tian, Guangwei Xu, Jian-Yu Li, Lang Wen, Dan-Lei Bi, F. Gao, Yong Shen, Guang-Ming Huang

Talanta: The International Journal of Pure and Applied Analytical Chemistry

2022

Rapid Profiling of Metabolic Perturbations to Antibiotics in Living Bacteria by Induced Electrospray Ionization Mass Spectrometry.

Liujuan Zhan, Zhuanghao Hou, Yu Wang, Huimin Liu, Yang-Zhong Liu, Guang-Ming Huang

Journal of the American Society for Mass Spectrometry

2022

α-Synuclein as a Target for Metallo-Anti-Neurodegenerative Agents.

Kai-Ming Cao, Yang Zhu, Zhuanghao Hou, Man-Man Liu, Yan-Yan Yang, Hong-Ze Hu, Yi Dai, Yu Wang, Si-Ming Yuan, Guang-Ming Huang, Jia-Ming Mei, P. Sadler, Yang-Zhong Liu

Angewandte Chemie

2021

Automatic Registration of the Mass Spectrometry Imaging Data of Sagittal Brain Slices to the Reference Atlas.

Shuangshuang Tian, Zhuanghao Hou, Xin Zuo, Wei Xiong, Guang-Ming Huang

Journal of the American Society for Mass Spectrometry

2021

Rapid desalting during electrospray ionization mass spectrometry for investigating protein-ligand interactions in the presence of concentrated salts.

Yuting Chen, Si-Ming Yuan, Yang-Zhong Liu, Guang-Ming Huang

Analytica Chimica Acta

2021

Bicarbonate buffers can promote crosslinking and alternative gas-phase dissociation pathways for multiprotein complexes

Gongyu Li, Junfeng Huang, Zhen Zheng, Qinjingwen Cao, Yuwei Tian, Guang-Ming Huang, Ling-Jun Li, B. Ruotolo

2021

Suppression of Protein Structural Perturbations in Native Electrospray Ionization during the Final Evaporation Stages Revealed by Molecular Dynamics Simulations.

Mou-Jun Luan, Zhuanghao Hou, Guang-Ming Huang

Journal of Physical Chemistry B

2021

Metabolomic profiling of single enlarged lysosomes

Hongying Zhu, Qianqian Li, Tie-Peng Liao, Xiang Yin, Qi Chen, Ziyi Wang, M. Dai, Lin Yi, Siyuan Ge, Chenjian Miao, Wen-Ping Zeng, Lili Qu, Z. Ju, Guang-Ming Huang, Chunlei Cang, Wei Xiong

Nature Methods

2020

感应交流电延缓nESI中模拟生物样品引发的毛细管堵塞(英文)

任梦婷,黄光明

《中国科学技术大学学报》

2020

三氟乙酸增强的解吸电喷雾电离质谱成像用于脂质空间分布研究

王晓群,黄光明

《高等学校化学学报》

2020

水凝胶涂覆纸喷雾离子化用于生物流体样品直接质谱分析

何元毅,詹柳娟,黄光明

《分析测试学报》

2020

Introducing charge tag via click reaction in living cells for single cell mass spectrometry†

Meihui Zhuang, Zhuanghao Hou, Peiyao Chen, G. Liang, Guang-Ming Huang

Chemical Science

2020

Protein precipitation coupled to paper spray with a tube for one-step analysis of blood.

Meng-Syuan Dai, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2020

Agarose hydrogel-enhanced paper spray ionization mass spectrometry for metabolite detection in raw urine.

Liujuan Zhan, Zhuanghao Hou, Guang-Ming Huang

In Analysis

2019

单细胞代谢物的质谱分析及其在单个神经元分析中的应用

侯壮豪,詹柳娟,栾谋君,田双双,戴梦杰,黄光明

《分析化学》

2019

In situ analysis of unsaturated fatty acids in human serum by negative-ion paper spray mass spectrometry.

Ling-Zhong Wan, G. Gong, Hao Liang, Guang-Ming Huang

Analytica Chimica Acta

2019

Covalent versus Non-covalent Binding of Ruthenium η6-p-Cymene Complexes to Zinc-finger Protein NCp7.

Yaping Sheng, Zhuanghao Hou, Shiyong Cui, Kai-Ming Cao, Si-Ming Yuan, Mei Sun, Jakob Kljun, Guang-Ming Huang, I. Turel, Yang-Zhong Liu

Chemistry

2019

Ultrafast Micro-Electrophoresis: Behind Direct Mass Spectrometry Measurements of Proteins and Metabolites in Living Cell/Cells.

Yuting Chen, Gongyu Li, Si-Ming Yuan, Yang Pan, Yang-Zhong Liu, Guang-Ming Huang

Analytical Chemistry

2019

Charge-dependent modulation of specific and nonspecific protein-metal ion interactions in nanoelectrospray ionization mass spectrometry.

Shihui Zheng, Si-Ming Yuan, Zhuanghao Hou, Gongyu Li, Yuting Chen, Yang Pan, Yang-Zhong Liu, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2019

High-throughput paper spray mass spectrometry via induced voltage.

Hai-Jing Zhu, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2019

Tetrathiomolybdate induces dimerization of the metal-binding domain of ATPase and inhibits platination of the protein

Tiantian Fang, Wanbiao Chen, Yaping Sheng, Si-Ming Yuan, Qiao-Wei Tang, Gongyu Li, Guang-Ming Huang, Ji-Hu Su, Xuan Zhang, J. Zang, Yang-Zhong Liu

Nature Communications

2018

Enhanced Desorption Electrospray Ionization Mass Spectrometry via Synchronizing Ion Generation and Ion Injection

Zhuanghao Hou, Xingchuang Xiong, Xiang Fang, Guang-Ming Huang

Journal of the American Society for Mass Spectrometry

2018

Selective Targeting of the Zinc Finger Domain of HIV Nucleocapsid Protein NCp7 with Ruthenium Complexes.

Yaping Sheng, Kai-Ming Cao, Ji Li, Zhuanghao Hou, Si-Ming Yuan, Guang-Ming Huang, Hong-Ke Liu, Yang-Zhong Liu

Chemistry

2018

In Situ Living Cell Protein Analysis by Single-Step Mass Spectrometry.

Gongyu Li, Si-Ming Yuan, Shihui Zheng, Yang-Zhong Liu, Guang-Ming Huang

Analytical Chemistry

2018

Time-resolved method to distinguish protein/peptide oxidation during electrospray ionization mass spectrometry.

Jiying Pei, Cheng-Chih Hsu, Ke-Fu Yu, Ying-Hui Wang, Guang-Ming Huang

Analytica Chimica Acta

2018

Reliable Tracking In-Solution Protein Unfolding via Ultrafast Thermal Unfolding/Ion Mobility-Mass Spectrometry.

Gongyu Li, Shihui Zheng, Yuting Chen, Zhuanghao Hou, Guang-Ming Huang

Analytical Chemistry

2018

Arsenic trioxide preferentially binds to the ring finger protein PML: understanding target selection of the drug.

Kai-Ming Cao, Yaping Sheng, Shihui Zheng, Si-Ming Yuan, Guang-Ming Huang, Yang-Zhong Liu

Metallomics

2018

A facile approach to improve the spray time and stability of paper spray ionization mass spectrometry with a Teflon tube

Yuan Cao, Guang-Ming Huang

2017

Ion suppression effect in desorption electrospray ionization and electrospray ionization mass spectrometry.

Zheng Wang, Hai-Jing Zhu, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2017

The Effect of Salts in Promoting Specific and Competitive Interactions between Zinc Finger Proteins and Metals

Gongyu Li, Si-Ming Yuan, Shihui Zheng, Yuting Chen, Zhen Zheng, Yang-Zhong Liu, Guang-Ming Huang

Journal of the American Society for Mass Spectrometry

2017

Unexpected Reduction of Iminoquinone and Quinone Derivatives in Positive Electrospray Ionization Mass Spectrometry and Possible Mechanism Exploration

Jiying Pei, Cheng-Chih Hsu, Rui-Jie Zhang, Ying-Hui Wang, Ke-Fu Yu, Guang-Ming Huang

Journal of the American Society for Mass Spectrometry

2017

Nanocoating cellulose paper based microextraction combined with nanospray mass spectrometry for rapid and facile quantitation of ribonucleosides in human urine.

Ling-Zhong Wan, Hai-Jing Zhu, Ya-Feng Guan, Guang-Ming Huang

Talanta: The International Journal of Pure and Applied Analytical Chemistry

2017

Single-neuron identification of chemical constituents, physiological changes, and metabolism using mass spectrometry

Hongying Zhu, Gui-Chang Zou, Ning Wang, Meihui Zhuang, Wei Xiong, Guang-Ming Huang

Proceedings of the National Academy of Sciences of the United States of America

2016

纸芯片用于比色法筛查和质谱鉴定的研究(英文)

刘蒙蒙,黄光明

《中国科学技术大学学报》

2016

Binding States of Protein-Metal Complexes in Cells.

Gongyu Li, Si-Ming Yuan, Yang Pan, Yang-Zhong Liu, Guang-Ming Huang

Analytical Chemistry

2016

Mechanistic study of CBT-Cys click reaction and its application for identifying bioactive N-terminal cysteine peptides in amniotic fluid

Zhen Zheng, Peiyao Chen, Gongyu Li, Yun-Xia Zhu, Zhong-Hua Shi, Yu Luo, Chun Zhao, Ziyi Fu, Xian-Wei Cui, Chen-Bo Ji, Fuqiang Wang, Guang-Ming Huang, G. Liang

Chemical Science

2016

Sheathless interface to match flow rate of capillary electrophoresis with electrospray mass spectrometry using regular-sized capillary.

Yue Yin, Gongyu Li, Ya-Feng Guan, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2015

Reactive paper spray mass spectrometry for in situ identification of quinones.

Xuan Zhou, Jiying Pei, Guang-Ming Huang

Rapid Communications in Mass Spectrometry

2015

Alleviation of electrochemical oxidation for peptides and proteins in electrospray ionization: obtaining more accurate mass spectra with induced high voltage.

Jiying Pei, Xuan Zhou, Xiaoqun Wang, Guang-Ming Huang

Analytical Chemistry

2015

Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry.

Gongyu Li, Jiying Pei, Yue Yin, Guang-Ming Huang

In Analysis

2014

Increased disulfide peptide sequence coverage via “cleavage ON/OFF” switch during nanoelectrospray

Gongyu Li, Yue Yin, Guang-Ming Huang

2014

Reactive intermediate detection in real time via paper assisted thermal ionization mass spectrometry

Jiying Pei, Yan‐Biao Kang, Guangming Huang

The Analyst

2012

Gas-flow assisted ion transfer for mass spectrometry.

Sandilya V B Garimella, Wei Xu, Guang-Ming Huang, J. Harper, R. Cooks, Z. Ouyang

Journal of Mass Spectrometry

2012

Rapid detection of urushiol allergens of Toxicodendron genus using leaf spray mass spectrometry.

Fatkhulla K. Tadjimukhamedov, Guang-Ming Huang, Z. Ouyang, R. Cooks

In Analysis

2011

New ionization methods and miniature mass spectrometers for biomedicine: DESI imaging for cancer diagnostics and paper spray ionization for therapeutic drug monitoring

R. Cooks, Nicholas E. Manicke, A. Dill, D. Ifa, Livia S. Eberlin, Anthony B. Costa, He Wang, Guang-Ming Huang, Z. Ouyang

Faraday discussions

2011

Induced nanoelectrospray ionization for matrix-tolerant and high-throughput mass spectrometry.

Guang-Ming Huang, Guang-Tao Li, R. Cooks

Angewandte Chemie

2011

Synchronized inductive desorption electrospray ionization mass spectrometry.

Guang-Ming Huang, Guang-Tao Li, J. Ducan, Z. Ouyang, R. Cooks

Angewandte Chemie

2007

Rapid screening of anabolic steroids in urine by reactive desorption electrospray ionization.

Guang-Ming Huang, Hao Chen, Xin-Rong Zhang, R. Cooks, Z. Ouyang

Analytical Chemistry

会议论文

2019

电喷雾中的电泳效应及其在代谢物和蛋白质的原位质谱分析中的应用

黄光明

《中国化学会第22届全国色谱学术报告会及仪器展览会论文集(第一卷)》

2018

活体单细胞质谱分析方法研究

黄光明

《2018年中国质谱学术大会(CMSC 2018)论文集》

2018

基于单细胞质谱研究单个神经元内物质和生理变化

朱洪影,黄光明

《2018年中国质谱学术大会(CMSC 2018)论文集》

2018

靶向砷化合物与环指蛋白/锌指蛋白相互作用的质谱研究

侯壮豪,曹开明,刘扬中,黄光明

《2018年中国质谱学术大会(CMSC 2018)论文集》

2018

活体单细胞质谱分析

黄光明,朱洪影,李功玉,陈玉婷,侯壮豪

《中国化学会第十三届全国分析化学年会论文集(二)》

2018

通孔板辅助的同步解吸附离子化电喷雾质谱成像

侯壮豪,熊行创,方向,黄光明

《中国化学会第十三届全国分析化学年会》

2018

基于单细胞质谱研究单个神经元内物质和生理变化

朱洪影,黄光明,San Zhang

《中国化学会第十三届全国分析化学年会论文集(二)》

2018

Mass Spectrometry Imaging of Brain Cholesterol and Metabolites with Trifluoroacetic Acid-Enhanced Desorption Electrospray Ionization

Xiaoqun Wang, Yiwen Hou, Zhuanghao Hou, Wei Xiong, Guangming Huang

《2018年中国质谱学术大会(CMSC 2018)论文集》

2017

单细胞质谱分析用于活体神经细胞的代谢通路研究

黄光明

《第三届全国质谱分析学术报告会摘要集》

2017

降低纳升电喷雾的毛细管尖端堵塞现象的研究

任梦婷,黄光明

《第三届全国质谱分析学术报告会摘要集-分会场1:新仪器新技术》

2017

非接触式纸喷雾离子源离子抑制效应的研究

朱海晶,黄光明

《第三届全国质谱分析学术报告会摘要集-分会场3:新型离子源》

2017

离子发生与离子注入过程同步捕捉实现解吸附电喷雾离子化质谱信号增强研究

侯壮豪,方向,黄光明

《第三届全国质谱分析学术报告会摘要集-分会场3:新型离子源》

2017

同步辐射光解离分析蛋白质气相结构和电荷态的关系

郑师慧,黄光明

《第三届全国质谱分析学术报告会摘要集-分会场5:有机/生物质谱新方法》

2015

复杂生物体系中蛋白质的快速检测

黄光明,李功玉

《中国化学会第二届全国质谱分析学术报告会会议摘要集》

2015

新型无鞘液毛细管电泳-质谱联用接口的研究

黄光明,殷跃

《中国化学会第二届全国质谱分析学术报告会会议摘要集》

2015

感应纳升电喷雾质谱直接分析单细胞

黄光明,朱洪影

《中国化学会第二届全国质谱分析学术报告会会议摘要集》

2015

原位质谱在细胞分析中的应用研究

黄光明

《中国化学会第二届全国质谱分析学术报告会会议摘要集》

2015

纸芯片与质谱联用药物监测的研究

黄光明,刘蒙蒙

《中国化学会第二届全国质谱分析学术报告会会议摘要集》

2015

MILLISECOND MICRO-ELECTROPHORESIS MASS SPECTROMETRY OF PROTEINS IN COMPLEX MIXTURES

Guangming Huang, Gongyu Li

《第十六届北京分析测试学术报告会暨展览会摘要集》

2014

交流感应辅助超声雾化离子源对生物大分子的软电离质谱分析

黄光明,李攻玉

《中国化学会第29届学术年会摘要集——第38分会:质谱分析》

预印本

2025

Coffee Ring Effect-Enhanced Desorption Electrospray Ionization Mass Spectrometry for Drugs and Metabolite Detection in Raw Urine

Baoyou Chu, Liujuan Zhan, Guangming Huang

SSRN Electronic Journal

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