高仁钧｜吉林大学｜学者主页

2026

Unleashing the industrial potential of microbial plant cell wall polysaccharide-degrading enzymes: recent advances in protein engineering

Zihao Huang, Liangtao Xu, Jun Zhou, Renjun Gao, 石怀彬

Frontiers in Microbiology

DOI

2026

Consensus design and in silico screening of GH42 β-galactosidase for enhanced catalytic hydrolysis of whey lactose

Simeng Ding, Jiajia Li, Jiahui Li, Huayu Nie, Yongxin Li, Zheng Guo, Renjun Gao

Journal of Dairy Science

DOI

2026

Regio-Stereoselective Synthesis of Galactosyl- sn -2-glycerol by Thermotoga naphthophila Glycosidase-Catalyzed Transglycosylation in a Cosolvent-Mediated System: Kinetic and Thermodynamic Insights

Jiabao Lyu, Simeng Ding, Christian Daugaard Wolff, Renjun Gao, Zheng Guo

ACS Sustainable Chemistry & Engineering

DOI

2026

Metal–Organic Framework Encapsulation of Ferulic Acid Decarboxylase: Robust Biocomposites Catalyzing Synthetically Useful Decarboxylation and Synergized CO 2 Fixation Reactions

Fuli Wang, Frederik Vig Benfeldt, Zeynab Khazaee, Mads Lund Nygaard Nielsen, Melissa Jane Marks, Yi Ouyang, Nina Lock, Renjun Gao, Zheng Guo, Bekir Engin Eser

ACS Sustainable Chemistry & Engineering

DOI

2025

Enhancement of Activity of Thermophilic Inorganic Pyrophosphatase Ton1914 via Site-Directed Mutagenesis

Siyao Liu, Xinrui Yang, Renjun Gao, Guiqiu Xie

Biomolecules

DOI

2025

Design of a recombinant Pseudomonas strain with enhanced degradation viability towards catecholic compounds by reconstituting EDO-hydrolysis pathway

Zihao Huang, Yingdan Piao, Xiemuxinuer Abuduwupuer, Simeng Ding, Tian Chen, Song Jiang, Ya Ding, Zheng Guo, Renjun Gao

Journal of environmental chemical engineering

DOI

2025

Enhancement of Enzyme Activity by Alternating Magnetic Field and Near-Infrared Irradiation

Fang Wang, Yuchen Liu, Qikai Dong, Zihan Li, Senrong Liang, Tianyi Zhang, Liangtao Xu, Renjun Gao

Catalysts

DOI

2024

扩展生物催化的边界：嗜热酶的潜力与应用

李雅静，李悦君，杨昕睿，解桂秋，高仁钧

《生命的化学》

2024

Regulate catalytic performance by engineering non-regular structure of extradiol dioxygenase: An entrance to bottom strategy

Zihao Huang, Deyuan Qin, Xiemuxinuer Abuduwupuer, Luxin Cao, Yingdan Piao, Zhengkang Shao, Liyan Jiang, Zheng Guo, Renjun Gao

International Journal of Biological Macromolecules

DOI

2024

Unlocking Industrial Potential: Phase-Transition Coimmobilization of Multienzyme Systems for High-Efficiency Uridine Diphosphate Galactose Production

Yajing Li, Xinrui Yang, Qi Chen, Yuejun Li, Renjun Gao

Journal of Agricultural and Food Chemistry

DOI

2024

The Improved Antineoplastic Activity of Thermophilic L-Asparaginase Tli10209 via Site-Directed Mutagenesis

Lijuan Zhang, Simeng Ding, Xiuhui Tang, Renjun Gao, Rui Huo, Guiqiu Xie

Biomolecules

DOI

2024

Engineering non-conservative substrate recognition sites of extradiol dioxygenase: Computation guided design to diversify and accelerate degradation of aromatic compounds

Zihao Huang, Zhenyu Gu, Xiemuxinuer Abuduwupuer, Deyuan Qin, Yuchen Liu, Zheng Guo, Renjun Gao

International Journal of Biological Macromolecules

DOI

2024

Efficient One-Pot Synthesis of Uridine Diphosphate Galactose Employing a Trienzyme System

Yajing Li, Qi Chen, Siyao Liu, Lin Deng, Shichao Li, Renjun Gao

Journal of Agricultural and Food Chemistry

DOI

2024

Selective enhanced cytotoxicity of amino acid deprivation for cancer therapy using thermozyme functionalized nanocatalyst

Xiuhui Tang, Lijuan Zhang, Mingwang Huang, Fang Wang, Guiqiu Xie, Rui Huo, Renjun Gao

Journal of Nanobiotechnology

DOI

2024

NIR-accelerated cascade reaction for degradation of organophosphorus compounds by Au/PTE/ZIF-8: cooperative effect and mechanism

Fang Wang, Yi Ouyang, Pengfei Zhou, Yan Zhang, Renjun Gao, Bekir Engin Eser, Zheng Guo

Catalysis Science & Technology

DOI

2023

Increased Expression Levels of Thermophilic Serine Protease TTHA0724 through Signal Peptide Screening in Bacillus subtilis and Applications of the Enzyme

Yiwen Xu, X. H. Xuan, Renjun Gao, Guiqiu Xie

International Journal of Molecular Sciences

DOI

2023

Selective and Sustainable Production of Sub‐terminal Hydroxy Fatty Acids by a Self‐Sufficient CYP102 Enzyme from Bacillus Amyloliquefaciens

Li Zong, Yan Zhang, Zhengkang Shao, Anita Ljubić, Charlotte Jacobsen, Renjun Gao, Bekir Engin Eser, Yingwu Wang, Zheng Guo

ChemBioChem

DOI

2022

Site-Specific Covalent Immobilization of Methylobacterium extorquens Non-Blue Laccse Melac13220 on Fe3O4 Nanoparticles by Aldehyde Tag

Abidan Ainiwaer, Ao Li, Zhao Xingwang, Yujiao Xu, Si‐ping Han, Renjun Gao

Catalysts

DOI

2022

Thermophilic Inorganic Pyrophosphatase Ton1914 from Thermococcus onnurineus NA1 Removes the Inhibitory Effect of Pyrophosphate

Yajing Li, Xue Na Yang, Renjun Gao

International Journal of Molecular Sciences

DOI

2022

Characterization of a Novel Fe2+ Activated Non-Blue Laccase from Methylobacterium extorquens

Abidan Ainiwaer, Yue Liang, Xiao Ye, Renjun Gao

International Journal of Molecular Sciences

DOI

2022

Superanionic Solvent‐Free Liquid Enzymes Exhibit Enhanced Structures and Activities

Ye Zhou, Jannik Nedergaard Pedersen, Jacob Nedergaard Pedersen, Nykola C. Jones, Søren Vrønning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Willis Perriman, Renjun Gao, Zheng Guo

Advanced Science

DOI

2022

Molecular dynamics simulation guided distal mutation of Thermotoga naphthophila β-glucosidase for significantly enhanced synthesis of galactooligosaccharides and expanded product scope

Jiabao Lyu, Jian Zhang, Jingxuan Zhu, Siao Chen, Tao Han, Yan Zhang, Renjun Gao, Guiqiu Xie, Zheng Guo

International Journal of Biological Macromolecules

DOI

2022

Current Strategies for Real-Time Enzyme Activation

Fang Wang, Yuchen Liu, Chang Du, Renjun Gao

Biomolecules

DOI

2021

A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants

Xiaowen Zhang, Zihao Huang, Dan Wang, Yan Zhang, Bekir Engin Eser, Zhenyu Gu, Rongrong Dai, Renjun Gao, Zheng Guo

Journal of Hazardous Materials

DOI

2021

Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids

Li Zong, Yan Zhang, Zhengkang Shao, Yingwu Wang, Zheng Guo, Renjun Gao, Bekir Engin Eser

Catalysts

DOI

2021

Galactosyldiacylglycerols: From a Photosynthesis-Associated Apparatus to Structure-Defined In Vitro Assembling

Jiabao Lyu, Renjun Gao, Zheng Guo

Journal of Agricultural and Food Chemistry

DOI

2020

Characterization and modification of two self-sufficient CYP102 family enzymes from Bacillus amyloliquefaciens DSM 7 with distinct regioselectivity towards fatty acid hydroxylation

Li Zong, Renjun Gao, Zheng Guo, Zhengkang Shao, Yingwu Wang, Bekir Engin Eser

Biochemical Engineering Journal

DOI

2020

Design and Construction of an Effective Expression System with Aldehyde Tag for Site-Specific Enzyme Immobilization

Fang Wang, Rong Li, Hui Jian, Zihao Huang, Yingwu Wang, Zheng Guo, Renjun Gao

Catalysts

DOI

2019

Covalent immobilization of Bacillus subtilis lipase A on Fe3O4 nanoparticles by aldehyde tag: An ideal immobilization with minimal chemical modification

Jiabao Lyu, Zidan Li, Juncheng Men, Ruyun Jiang, Guilin Tang, Ye Zhou, Renjun Gao

Process Biochemistry

DOI

2019

“Bridge regions” regulate catalysis and protein stability of acylpeptide hydrolase

Dongni Liu, Lin Deng, Dan Wang, Wei Li, Renjun Gao

Biochemical Engineering Journal

DOI

2019

Insight into the Structure and Activity of Surface‐Engineered Lipase Biofluids

Ye Zhou, Nykola C. Jones, Jannik Nedergaard Pedersen, Bianca C. Pérez, Søren Vrønning Hoffmann, Steen Vang Petersen, Jan Skov Pedersen, Adam Willis Perriman, Peter Kjær Kristensen, Renjun Gao, Zheng Guo

ChemBioChem

DOI

2019

Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer's disease

Dongni Liu, Wei Li, Xiaoyu Jiang, Shuju Bai, Jiyang Liu, Xutong Liu, Yuhua Shi, Ziyu Kuai, Wei Kong, Renjun Gao, Yaming Shan

Theranostics

DOI

2019

Real-time regulation of catalysis by remote-controlled enzyme-conjugated gold nanorod composites for aldol reaction-based applications

Wei Li, Dongni Liu, Xu Geng, Zhengqiang Li, Renjun Gao

Catalysis Science & Technology

DOI

2018

The additive mutational effects from surface charge engineering: A compromise between enzyme activity, thermostability and ionic liquid tolerance

Ye Zhou, Bianca C. Pérez, Weiwei Hao, Jiabao Lv, Renjun Gao, Zheng Guo

Biochemical Engineering Journal

DOI

2017

Enhanced Synthesis of Alkyl Galactopyranoside by Thermotoga naphthophila β-Galactosidase Catalyzed Transglycosylation: Kinetic Insight of a Functionalized Ionic Liquid-Mediated System

Jingwen Yang, Bianca C. Pérez, Sampson Anankanbil, Jingbo Li, Renjun Gao, Zheng Guo

ACS Sustainable Chemistry & Engineering

DOI

2017

嗜热糖苷酶与酶催化糖化合物合成研究进展

杨静文，孔繁思，栾霞，高仁钧

《广西科学》

2017

Valorizing Dairy Waste: Thermophilic Biosynthesis of a Novel Ascorbic Acid Derivative

Jingwen Yang, Bianca C. Pérez, Sampson Anankanbil, Jingbo Li, Ye Zhou, Renjun Gao, Zheng Guo

Journal of Agricultural and Food Chemistry

DOI

2017

β-Glucosidase from Thermotoga naphthophila RKU-10 for exclusive synthesis of galactotrisaccharides: Kinetics and thermodynamics insight into reaction mechanism

Jingwen Yang, Renjun Gao, Ye Zhou, Sampson Anankanbil, Jingbo Li, Guiqiu Xie, Zheng Guo

Food Chemistry

DOI

2017

Engineering T. naphthophila β-glucosidase for enhanced synthesis of galactooligosaccharides by site-directed mutagenesis

Jingwen Yang, Qi Wang, Ye Zhou, Jingbo Li, Renjun Gao, Zheng Guo

Biochemical Engineering Journal

DOI

2017

Synergistic effect of thermostable β-glucosidase TN0602 and cellulase on cellulose hydrolysis

Zhaoye Zhang, Man Wang, Renjun Gao, Xiaoxiao Yu, Guang Chen

3 Biotech

DOI

2017

Identification of an archaeal maltooligosyltrehalose trehalohydrolase encoded by an interrupted gene

Ye Zhou, Guiqiu Xie, Lin Chang, Yan Wang, Renjun Gao

Extremophiles

DOI

2016

Site-Specific, Covalent Immobilization of Dehalogenase ST2570 Catalyzed by Formylglycine-Generating Enzymes and Its Application in Batch and Semi-Continuous Flow Reactors

Hui Jian, Yingwu Wang, Yan Chao Bai, Rong Li, Renjun Gao

Molecules

DOI

2016

Mining catalytic promiscuity from Thermophilic archaea: an acyl-peptide releasing enzyme from Sulfolobus tokodaii (ST0779) for nitroaldol reactions

Xiaoxiao Yu, Bianca C. Pérez, Zhefei Zhang, Renjun Gao, Zheng Guo

Green Chemistry

DOI

2015

催化Aldol加成反应的新型枯草芽孢杆菌BS168酰基氨肽酶的表达和应用

于潇潇，王琦，周烨，高仁钧，王英武

《高等学校化学学报》

2015

嗜热古细菌Sulfolobus tokodaii脱卤酶在D-乳酸生产中的应用

解桂秋，潘东，何文龙，高贵，高仁钧

《高等学校化学学报》

2015

Microwave-assisted synthesis of butyl galactopyranoside catalyzed by β-galactosidase from Thermotoga naphthophila RKU-10

Jingwen Yang, Xiaochen Chen, Dahai Yu, Renjun Gao

Process Biochemistry

DOI

2015

Gene cloning and molecular characterization of a β-glucosidase from Thermotoga naphthophila RUK-10: an effective tool for synthesis of galacto-oligosaccharide and alkyl galactopyranosides

Fansi Kong, Jingwen Yang, Zhen Zhen, Tingting Liang, Dongliang Zhu, Renjun Gao, Guiqiu Xie

Chemical Research in Chinese Universities

DOI

2015

Characterization and mechanism insight of accelerated catalytic promiscuity of Sulfolobus tokodaii (ST0779) peptidase for aldol addition reaction

Rong Li, Bianca C. Pérez, Hui Jian, Mads Mørk Jensen, Renjun Gao, Mingdong Dong, Marianne Glasius, Zheng Guo

Applied Microbiology and Biotechnology

DOI

2015

Gene clone and characterization of a novel thermostable β-galactosidase with transglycosylation activity from Thermotoga naphthophila RUK-10

Jingwen Yang, Xiangjun Di, Man Wang, Renjun Gao

Chemical Research in Chinese Universities

DOI

2015

Acyl-peptide releasing enzyme from Sulfolobus tokodaii (ST0779) as a novel promiscuous biocatalyst for aldol addition

Rong Li, Bianca C. Pérez, Hui Jian, Renjun Gao, Mingdong Dong, Zheng Guo

Catalysis Communications

DOI

2014

Clone, purification and characterization of thermostable aminopeptidase ST1737 from Sulfolobus tokodaii

Xiaoxiao Yu, Weiwei Hao, Guiqiu Xie, Yingwu Wang, Renjun Gao

Chemical Research in Chinese Universities

DOI

2014

Cloning, purification, and characterization of inorganic pyrophosphatase from the hyperthermophilic archaea Pyrococcus horikoshii

Dongmei Lu, Guiqiu Xie, Renjun Gao

Protein Expression and Purification

DOI

2014

Cloning, purification and characterization of a thermostable β-galactosidase from Thermotoga naphthophila RUK-10

Fansi Kong, Yeqing Wang, Shugui Cao, Renjun Gao, Guiqiu Xie

Process Biochemistry

DOI

2013

杂合酶的研究现状与发展前景

韩镇，解桂秋，高仁钧

《中国生物制品学杂志》

2012

Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation

Renjun Gao, Mia Fiilsøe Falkeborg, Xuebing Xu, Zheng Guo

Applied Microbiology and Biotechnology

DOI

2011

Enhanced Activity and Enantioselectivity of a Hyperthermophilic Esterase from Archaeon Aeropyrum pernix K1 by Acetone Treatment

Fangdi Cong, Kezhi Xing, Renjun Gao, Shugui Cao, Guirong Zhang

Applied Biochemistry and Biotechnology

DOI

2009

定向进化提高枯草芽孢杆菌脂肪酶的活力

赵博，陶进，马吉胜，廉虹，王岩，常琳，高仁钧，曹淑桂

Chinese Journal of Catalysis

2008

古细菌Aeropyrum pernix K1超嗜热酯酶APE1547的稳定性

解桂秋，高仁钧，毕云枫，王中禹，刘娜，冯雁，曹淑桂

《高等学校化学学报》

2008

枯草杆菌脂肪水解酶LipA和LipB

赵博，高仁钧，廉虹，曹淑桂

《中国生物化学与分子生物学报》

2008

Cloning, expression and characterization of an organic solvent tolerant lipase from Pseudomonas fluorescens JCM5963

Aijun Zhang, Renjun Gao, Naibing Diao, Guiqiu Xie, Gui Gao, Shugui Cao

Journal of Molecular Catalysis B Enzymatic

DOI

2008

Enzymatic synthesis of cyclohexyl-α and β-d-glucosides catalyzed by α- and β-glucosidase in a biphase system

Ruijian Wang, Xueying Yang, Renjun Gao, Ye Yang, Xiaojuan Wang, Shugui Cao

Journal of Molecular Catalysis B Enzymatic

DOI

2007

酶的定向进化方法和应用

鲁静，高仁钧，张焱茹

《北方农业学报》

2007

超嗜热酯酶APE1547中特殊位置氢键对酶活力和热稳定性的影响

毕云枫，解桂秋，高仁钧，鲁静，曹淑桂

《高等学校化学学报》

2007

酶立体选择性的定向进化策略

周俊，王妍，高仁钧

《中国生物制品学杂志》

2006

Aeropyrum pernix K1磷脂酶A2的克隆、表达及其性质

卢冬梅，吴英松，解桂秋，高仁钧，王智，曹淑桂，冯雁

《微生物学通报》

2006

Study on the relationship between structure and enantioselectivity of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1

Guirong Zhang, Renjun Gao, Liangyu Zheng, Aijun Zhang, Yuanhong Wang, Qiuyan Wang, Yan Fang Feng, Shugui Cao

Journal of Molecular Catalysis B Enzymatic

DOI

2004

Crystal Structure of an Acylpeptide Hydrolase/Esterase from Aeropyrum pernix K1

Mark G. Bartlam, Ganggang Wang, Haitao Yang, Renjun Gao, Xiaodong Zhao, Guiqiu Xie, Shuigui Cao, Yan Fang Feng, Zihe Rao

Structure

DOI

2004

A novel phospholipase A2/esterase from hyperthermophilic archaeon Aeropyrum pernix K1

Baijing Wang, Dongmei Lu, Renjun Gao, Zhen Yang, Shugui Cao, Yan Fang Feng

Protein Expression and Purification

DOI

2004

Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeonPyrococcus horikoshiiOT3

Binbin Liu, Xuemei Li, Renjun Gao, Weihong Zhou, Guiqiu Xie, Mark G. Bartlam, Hai Pang, Yan Fang Feng, Zihe Rao

Acta Crystallographica Section D Biological Crystallography

DOI

2004

Crystal Structure of the Hyperthermophilic Inorganic Pyrophosphatase from the Archaeon Pyrococcus horikoshii

Binbin Liu, Mark G. Bartlam, Renjun Gao, Weihong Zhou, Hai Pang, Yiwei Liu, Yan Fang Feng, Zihe Rao

Biophysical Journal

DOI

2003

Cloning, purification and properties of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1

Renjun Gao, Yan Fang Feng, Kazuhiko Ishikawa, Hiroyasu Ishida, Susumu Ando, Yoshitsugu Kosugi, Shugui Cao

Journal of Molecular Catalysis B Enzymatic

DOI

2002

Crystallization and preliminary crystallographic analysis of acylamino-acid releasing enzyme from the hyperthermophilic archaeonAeropyrum pernix

Ganggang Wang, Renjun Gao, Yi Ding, Haitao Yang, Shugui Cao, Yan Fang Feng, Zihe Rao

Acta Crystallographica Section D Biological Crystallography

DOI

2002

Crystallization and preliminary X-ray analysis of recombinant histone HPhA from the hyperthermophilic archaeonPyrococcus horikoshiiOT3

Ti Li, Xin Ji, Fei Sun, Renjun Gao, Shugui Cao, Yan Fang Feng, Zihe Rao

Acta Crystallographica Section D Biological Crystallography

DOI

2001

有机相中脂肪酶催化拆分环氧丙醇的研究

王智，高仁钧，杨硕，由德林，刘志前，王艳茹，曹淑桂

《吉林大学学报(理学版)》

精选作品

期刊论文

Unleashing the industrial potential of microbial plant cell wall polysaccharide-degrading enzymes: recent advances in protein engineering

Consensus design and in silico screening of GH42 β-galactosidase for enhanced catalytic hydrolysis of whey lactose

Regio-Stereoselective Synthesis of Galactosyl- sn -2-glycerol by Thermotoga naphthophila Glycosidase-Catalyzed Transglycosylation in a Cosolvent-Mediated System: Kinetic and Thermodynamic Insights

Metal–Organic Framework Encapsulation of Ferulic Acid Decarboxylase: Robust Biocomposites Catalyzing Synthetically Useful Decarboxylation and Synergized CO 2 Fixation Reactions

Enhancement of Activity of Thermophilic Inorganic Pyrophosphatase Ton1914 via Site-Directed Mutagenesis

Design of a recombinant Pseudomonas strain with enhanced degradation viability towards catecholic compounds by reconstituting EDO-hydrolysis pathway

Enhancement of Enzyme Activity by Alternating Magnetic Field and Near-Infrared Irradiation

扩展生物催化的边界：嗜热酶的潜力与应用

Regulate catalytic performance by engineering non-regular structure of extradiol dioxygenase: An entrance to bottom strategy

Unlocking Industrial Potential: Phase-Transition Coimmobilization of Multienzyme Systems for High-Efficiency Uridine Diphosphate Galactose Production

The Improved Antineoplastic Activity of Thermophilic L-Asparaginase Tli10209 via Site-Directed Mutagenesis

Engineering non-conservative substrate recognition sites of extradiol dioxygenase: Computation guided design to diversify and accelerate degradation of aromatic compounds

Efficient One-Pot Synthesis of Uridine Diphosphate Galactose Employing a Trienzyme System

Selective enhanced cytotoxicity of amino acid deprivation for cancer therapy using thermozyme functionalized nanocatalyst

NIR-accelerated cascade reaction for degradation of organophosphorus compounds by Au/PTE/ZIF-8: cooperative effect and mechanism

Increased Expression Levels of Thermophilic Serine Protease TTHA0724 through Signal Peptide Screening in Bacillus subtilis and Applications of the Enzyme

Selective and Sustainable Production of Sub‐terminal Hydroxy Fatty Acids by a Self‐Sufficient CYP102 Enzyme from Bacillus Amyloliquefaciens

Site-Specific Covalent Immobilization of Methylobacterium extorquens Non-Blue Laccse Melac13220 on Fe3O4 Nanoparticles by Aldehyde Tag

Thermophilic Inorganic Pyrophosphatase Ton1914 from Thermococcus onnurineus NA1 Removes the Inhibitory Effect of Pyrophosphate

Characterization of a Novel Fe2+ Activated Non-Blue Laccase from Methylobacterium extorquens

Superanionic Solvent‐Free Liquid Enzymes Exhibit Enhanced Structures and Activities

Molecular dynamics simulation guided distal mutation of Thermotoga naphthophila β-glucosidase for significantly enhanced synthesis of galactooligosaccharides and expanded product scope

Current Strategies for Real-Time Enzyme Activation

A new thermophilic extradiol dioxygenase promises biodegradation of catecholic pollutants

Optimization and Engineering of a Self-Sufficient CYP102 Enzyme from Bacillus amyloliquefaciens towards Synthesis of In-Chain Hydroxy Fatty Acids

Galactosyldiacylglycerols: From a Photosynthesis-Associated Apparatus to Structure-Defined In Vitro Assembling

Characterization and modification of two self-sufficient CYP102 family enzymes from Bacillus amyloliquefaciens DSM 7 with distinct regioselectivity towards fatty acid hydroxylation

Design and Construction of an Effective Expression System with Aldehyde Tag for Site-Specific Enzyme Immobilization

Covalent immobilization of Bacillus subtilis lipase A on Fe3O4 nanoparticles by aldehyde tag: An ideal immobilization with minimal chemical modification

“Bridge regions” regulate catalysis and protein stability of acylpeptide hydrolase

Insight into the Structure and Activity of Surface‐Engineered Lipase Biofluids

Using near-infrared enhanced thermozyme and scFv dual-conjugated Au nanorods for detection and targeted photothermal treatment of Alzheimer's disease

Real-time regulation of catalysis by remote-controlled enzyme-conjugated gold nanorod composites for aldol reaction-based applications

The additive mutational effects from surface charge engineering: A compromise between enzyme activity, thermostability and ionic liquid tolerance

Enhanced Synthesis of Alkyl Galactopyranoside by Thermotoga naphthophila β-Galactosidase Catalyzed Transglycosylation: Kinetic Insight of a Functionalized Ionic Liquid-Mediated System

嗜热糖苷酶与酶催化糖化合物合成研究进展

Valorizing Dairy Waste: Thermophilic Biosynthesis of a Novel Ascorbic Acid Derivative

β-Glucosidase from Thermotoga naphthophila RKU-10 for exclusive synthesis of galactotrisaccharides: Kinetics and thermodynamics insight into reaction mechanism

Engineering T. naphthophila β-glucosidase for enhanced synthesis of galactooligosaccharides by site-directed mutagenesis

Synergistic effect of thermostable β-glucosidase TN0602 and cellulase on cellulose hydrolysis

Identification of an archaeal maltooligosyltrehalose trehalohydrolase encoded by an interrupted gene

Site-Specific, Covalent Immobilization of Dehalogenase ST2570 Catalyzed by Formylglycine-Generating Enzymes and Its Application in Batch and Semi-Continuous Flow Reactors

Mining catalytic promiscuity from Thermophilic archaea: an acyl-peptide releasing enzyme from Sulfolobus tokodaii (ST0779) for nitroaldol reactions

催化Aldol加成反应的新型枯草芽孢杆菌BS168酰基氨肽酶的表达和应用

嗜热古细菌Sulfolobus tokodaii脱卤酶在D-乳酸生产中的应用

Microwave-assisted synthesis of butyl galactopyranoside catalyzed by β-galactosidase from Thermotoga naphthophila RKU-10

Gene cloning and molecular characterization of a β-glucosidase from Thermotoga naphthophila RUK-10: an effective tool for synthesis of galacto-oligosaccharide and alkyl galactopyranosides

Characterization and mechanism insight of accelerated catalytic promiscuity of Sulfolobus tokodaii (ST0779) peptidase for aldol addition reaction

Gene clone and characterization of a novel thermostable β-galactosidase with transglycosylation activity from Thermotoga naphthophila RUK-10

Acyl-peptide releasing enzyme from Sulfolobus tokodaii (ST0779) as a novel promiscuous biocatalyst for aldol addition

Clone, purification and characterization of thermostable aminopeptidase ST1737 from Sulfolobus tokodaii

Cloning, purification, and characterization of inorganic pyrophosphatase from the hyperthermophilic archaea Pyrococcus horikoshii

Cloning, purification and characterization of a thermostable β-galactosidase from Thermotoga naphthophila RUK-10

杂合酶的研究现状与发展前景

Production of sophorolipids with enhanced volumetric productivity by means of high cell density fermentation

Enhanced Activity and Enantioselectivity of a Hyperthermophilic Esterase from Archaeon Aeropyrum pernix K1 by Acetone Treatment

定向进化提高枯草芽孢杆菌脂肪酶的活力

古细菌Aeropyrum pernix K1超嗜热酯酶APE1547的稳定性

枯草杆菌脂肪水解酶LipA和LipB

Cloning, expression and characterization of an organic solvent tolerant lipase from Pseudomonas fluorescens JCM5963

Enzymatic synthesis of cyclohexyl-α and β-d-glucosides catalyzed by α- and β-glucosidase in a biphase system

酶的定向进化方法和应用

超嗜热酯酶APE1547中特殊位置氢键对酶活力和热稳定性的影响

酶立体选择性的定向进化策略

Aeropyrum pernix K1磷脂酶A2的克隆、表达及其性质

Study on the relationship between structure and enantioselectivity of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1

Crystal Structure of an Acylpeptide Hydrolase/Esterase from Aeropyrum pernix K1

A novel phospholipase A2/esterase from hyperthermophilic archaeon Aeropyrum pernix K1

Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeonPyrococcus horikoshiiOT3

Crystal Structure of the Hyperthermophilic Inorganic Pyrophosphatase from the Archaeon Pyrococcus horikoshii

Cloning, purification and properties of a hyperthermophilic esterase from archaeon Aeropyrum pernix K1

Crystallization and preliminary crystallographic analysis of acylamino-acid releasing enzyme from the hyperthermophilic archaeonAeropyrum pernix

Crystallization and preliminary X-ray analysis of recombinant histone HPhA from the hyperthermophilic archaeonPyrococcus horikoshiiOT3

有机相中脂肪酶催化拆分环氧丙醇的研究

会议论文

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Engineering Thermotoganaphthophila β-glucosidase for enhanced synthesis of galactooligosaccharides:improving innate transgalactosylation ability by single point mutation

Cloning,expression and characterization of a recombinant thermophilic β-galactosidase from Dictyoglomus thermophilum H-6-12