乔洁｜武汉轻工大学｜学者主页

期刊论文

2026

Elimination of cis -cleavage in CRISPR diagnostics for one-pot rapid nucleic acid detection

Wenhao Yin, Zhili Jin, Qingyuan Jiang, Shuqi Jin, Xinping Wang, Ruyi He, Bin Qiao, Jie Qiao, Xianhua Zhang, Yi Liu

Nucleic Acids Research

DOI

2026

A One-Pot Reverse Transcriptase-Mediated, Pre-amplification-Free CRISPR/Cas12a Assay for Ultrasensitive Nucleic Acid Detection

Ruyi He, Chenchen Zhang, Junqi Zhang, Kaiwen Zhang, Wenhao Yin, Bin Qiao, Luyi Zhang, Shuo Zhang, Jie Qiao, Yi Liu

ACS Sensors

DOI

2025

Direct preparation of Cas9 ribonucleoproteins with an extended ‘gRNA-shRNA’ construct in Escherichia coli for precise genome manipulation

Jie Qiao, Wenli Sun, Wenhao Yin, Li Hu, Xinping Wang, Yi Liu

International Journal of Biological Macromolecules

DOI

2025

Split CRISPR/Cas systems: Pioneering solutions for molecular diagnostics challenges.

Junqi Zhang, Meng-Jia Zhu, Hongbin Yan, Jie Qiao, Yi Liu

Biosensors & bioelectronics

DOI

2025

Boosting CRISPR/Cas12a intrinsic RNA detection capability through pseudo hybrid DNA–RNA substrate design

Jie Qiao, Junqi Zhang, Qingyuan Jiang, Shuqi Jin, Ruyi He, Bin Qiao, Yi Liu

Nucleic Acids Research

DOI

2025

CRISPR/Cas12a DTR system: a topology-guided Cas12a assay for specific dual detection of RNA and DNA targets

Qingyuan Jiang, Shuqi Jin, Z. Qin, Junqi Zhang, Ruyi He, Zhuo Chen, Bin Qiao, Jie Qiao, Yi Liu

Nucleic Acids Research

DOI

2024

Recent advances in spatiotemporal control of the CRISPR/Cas9 system

Junqi Zhang, Yuzi Zhou, Jie Qiao, Yi Liu

Colloids and Surfaces B Biointerfaces

DOI

2024

Split crRNA with CRISPR-Cas12a enabling highly sensitive and multiplexed detection of RNA and DNA

Yi-Chuan Chen, Xinping Wang, Junqi Zhang, Qingyuan Jiang, Bin Qiao, Baoxia He, Wenhao Yin, Jie Qiao, Yi Liu

Nature Communications

DOI

2024

Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay

Junqi Zhang, Wenhao Yin, Qingyuan Jiang, Wuxiang Mao, Wenwen Deng, Shuqi Jin, Xinping Wang, Ruyi He, Jie Qiao, Yi Liu

bioRxiv

DOI

2023

An amplification-free CRISPR/Cas12a-based fluorescence assay for ultrasensitive detection of nuclease activity

Xinping Wang, Yi-Chuan Chen, Lixin Ma, Zhenwei Han, Yi Liu, Jie Qiao

Talanta

DOI

2022

Strategies for Enhancing the Homology-Directed Repair Efficiency of CRISPR-Cas Systems

Wenli Sun, Hui Liu, Wenhao Yin, Jie Qiao, Xueke Zhao, Yi Liu

The CRISPR Journal

DOI

2021

CRISPR/dCas9系统在活细胞成像领域的研究进展

林斯雨，钟星，马立新，乔洁，刘奕

《生物工程学报》

DOI

2020

基于新的CRISPR/Cas9技术实现斑马鱼LHX9基因的快速编辑及对F0突变体性腺发育的初筛

王楠，朱惠，朱文娇，张昌润，沈也雷，董梅，宋怀东，乔洁

《现代生物医学进展》

DOI

2020

Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction

Si Wang, Zheying Min, Qianzhao Ji, Lingling Geng, Yao Su, Zunpeng Liu, Huifang Hu, Lixia Wang, Weiqi Zhang, Keiichiro Suzuiki, Yu Huang, Puyao Zhang, Tie-Shan Tang, Jing Qu, Yang Yu, Guang-Hui Liu, Jie Qiao

Protein & Cell

2019

Exposure to an enriched environment promotes the terminal maturation and proliferation of natural killer cells in mice

Zihong Meng, Tingting Liu, Yanfang Song, Qing Wang, Dengfei Xu, Jinghui Jiang, Mengge Li, Jie Qiao, Xiaoying Luo, Jianren Gu, Hong Tu, Yu Gan

Brain Behavior And Immunity，Vol. 77

DOI

2017

CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs

Lixia Wang, Fei Yi, Lina Fu, Jiping Yang, Si Wang, Zhaoxia Wang, Keiichiro Suzuki, Liang Sun, Xiuling Xu, Yang Yu, Jie Qiao, Juan Carlos Izpisua Belmonte, Ze Yang, Yun Yuan, Jing Qu, Guang-Hui Liu

Protein & Cell

会议论文

2019

Streamlines production of Cas9 RNPs in Escherichia coli and their biological applications

Jie Qiao, Wenli Sun, Siyu Lin, Yi Liu

《中国化学会第六届全国生物物理化学会议（NCBPC-6）摘要集》

DOI

2019

Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein

Jie Qiao, Wenli Sun, Siyu Lin, Rong Jin, Lixin Ma, Yi Liu

《第十一届全国化学生物学学术会议论文摘要（第三卷）》

DOI

2019

Co-expression of Cas9 and single guided RNAs in Escherichia coli fordirect production of Cas9 RNP

Jie Qiao, Wenqiang Li, Siyu Lin, Lixin Ma, Yi Liu

《第十一届全国化学生物学学术会议论文摘要（第三卷）》

DOI

2019

Streamlines production of Cas9 RNPs in Escherichia coli and their biological applications

Jie Qiao, Wenli Sun, Siyu Lin, Yi Liu

《第十一届全国化学生物学学术会议论文摘要（第三卷）》

DOI

预印本

2022

Rational design of Cas9 ribonucleoprotein with a “gRNA-shRNA” for multidimensional genome manipulation and enhanced homology-directed repair

Jie Qiao, Wenli Sun, Wenhao Yin, Lixin Ma, Yi Liu

bioRxiv (Cold Spring Harbor Laboratory)

DOI

乔洁

精选作品

期刊论文

Elimination of cis -cleavage in CRISPR diagnostics for one-pot rapid nucleic acid detection

A One-Pot Reverse Transcriptase-Mediated, Pre-amplification-Free CRISPR/Cas12a Assay for Ultrasensitive Nucleic Acid Detection

Direct preparation of Cas9 ribonucleoproteins with an extended ‘gRNA-shRNA’ construct in Escherichia coli for precise genome manipulation

Split CRISPR/Cas systems: Pioneering solutions for molecular diagnostics challenges.

Boosting CRISPR/Cas12a intrinsic RNA detection capability through pseudo hybrid DNA–RNA substrate design

CRISPR/Cas12a DTR system: a topology-guided Cas12a assay for specific dual detection of RNA and DNA targets

Recent advances in spatiotemporal control of the CRISPR/Cas9 system

Split crRNA with CRISPR-Cas12a enabling highly sensitive and multiplexed detection of RNA and DNA

Precise amplification-free detection of highly structured RNA with an enhanced SCas12a assay

An amplification-free CRISPR/Cas12a-based fluorescence assay for ultrasensitive detection of nuclease activity

Strategies for Enhancing the Homology-Directed Repair Efficiency of CRISPR-Cas Systems

CRISPR/dCas9系统在活细胞成像领域的研究进展

基于新的CRISPR/Cas9技术实现斑马鱼LHX9基因的快速编辑及对F0突变体性腺发育的初筛

Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction

Exposure to an enriched environment promotes the terminal maturation and proliferation of natural killer cells in mice

CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs

会议论文

Streamlines production of Cas9 RNPs in Escherichia coli and their biological applications

Cytosolic delivery of CRISPR/Cas9 ribonucleoproteins for genome editing using chitosan-coated red fluorescent protein

Co-expression of Cas9 and single guided RNAs in Escherichia coli fordirect production of Cas9 RNP

Streamlines production of Cas9 RNPs in Escherichia coli and their biological applications

预印本

Rational design of Cas9 ribonucleoprotein with a “gRNA-shRNA” for multidimensional genome manipulation and enhanced homology-directed repair

研究方向