高文运｜西北大学｜学者主页

2026

The regulatory subunit of acetohydroxyacid synthase enhances the catalysis of its catalytic subunit on 2-ketobutyrate

Zi‐Qi Zheng, Zhao-Tian Wei, Shi-Bo Jin, Zhenzhen Yao, Can-Xia Ma, Wen‐Yun Gao, Heng Li

Archives of Biochemistry and Biophysics

DOI

2025

Catalytic insights of Bacillus subtilis acetolactate decarboxylase

Zi‐Qi Zheng, Ze-Xin Niu, Zi-Dong Chen, Lei-Lei Chao, Qingyang Zhang, Xin-Yi Zhao, Zhaohui Wu, Wen‐Yun Gao, Heng Li

Molecular Catalysis

DOI

2024

Catalytic insights of acetolactate synthases from different bacteria

Yan‐Fei Liang, Ze-Xin Niu, Zhaohui Wu, Qingyang Zhang, Xin-Yi Zhao, Lei-Lei Chao, Heng Li, Wen‐Yun Gao

Archives of Biochemistry and Biophysics

DOI

2024

Impact of the key residues of 1-Deoxy-D-Xylulose-5-Phosphate synthase on its catalysis

Yan‐Fei Liang, Shuai Feng, Dan Wu, Meng Ma, Jin−Tao Ren, Qian Zhao, Wen‐Yun Gao, Heng Li

Molecular Catalysis

DOI

2024

Changes in α-Dicarbonyl Compound Contents during Storage of Various Fruits and Juices

Yang Yang, Xueyi Liu, Qian Zhao, Dan Wu, Jin-Tao Ren, Meng Ma, P. Li, Jia-Cai Wu, Wen‐Yun Gao, Heng Li

Foods

DOI

2024

Determination of α-Dicarbonyl compounds in traditional Chinese herbal medicines

Yang Yang, Hai-Ling Wang, Rui-Tong Cheng, Pei-Rong Zheng, Hui-Peng Sun, Zhiwen Liu, Heng Yuan, Xueyi Liu, Wen‐Yun Gao, Heng Li

Fitoterapia

DOI

2024

Re-investigation of in vitro activity of acetohydroxyacid synthase I holoenzyme from Escherichia coli

Hai-Ling Wang, Hui-Peng Sun, Pei-Rong Zheng, Rui-Tong Cheng, Zhiwen Liu, Heng Yuan, Wen‐Yun Gao, Heng Li

Archives of Biochemistry and Biophysics

DOI

2023

The Catalysis of Bacillus subtilis Acetolactate Synthase Is Fundamentally Underestimated

Guo-Fei Jin, Jin-Meng Tian, Xueyi Liu, Zi-Dong Chen, Le-Tian Yan, Shui-Hong Hua, Hui Liu, Huijuan Yang, Heng Li, Wen‐Yun Gao

Molecular Catalysis

DOI

2022

Overall analyses of the reactions catalyzed by acetohydroxyacid synthase/acetolactate synthase using a precolumn derivatization-HPLC method

Xian Hui, Jin-Meng Tian, Xin Wang, Zhenqian Zhang, Yamei Zhao, Wen‐Yun Gao, Heng Li

Analytical Biochemistry

DOI

2022

Characterization of acetolactate decarboxylase of Streptococcus thermophilus and its stereoselectivity in decarboxylation of α-hydroxy-β-ketoacids

Zi‐Qi Zheng, Cai-Yun Luo, Han Chen, Huan Sun, Xian Hui, Zi-Dong Chen, Wen‐Yun Gao, Heng Li

Bioorganic Chemistry

DOI

2021

Inhibitory Activity of Proanthocyanidins Against Escherichia coli 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase

Xian Hui, Bo-Rong Zhu, Longlong Wu, Wen‐Yun Gao, Yiming Li, Qi Jia, Heng Li

Natural Product Communications

DOI

2021

The chemical mechanisms of the enzymes in the branched-chain amino acids biosynthetic pathway and their applications

Yan‐Fei Liang, Zi-Xian Long, Ya-Jian Zhang, Cai-Yun Luo, Le-Tian Yan, Wen‐Yun Gao, Heng Li

Biochimie

DOI

2020

HPLC法同时测定膜苞鸢尾中两种有效成分

杨阳，路姣，赵长琦，高文运

《化学工程师》

2020

唾液酸检测方法研究进展

杨阳，焦淑玲，朱美旗，高文运

《化学工程师》

2020

HPLC法同时测定中亚鸢尾中3种抗炎活性成分的含量

杨阳，杨穗，赵长琦，高文运

《药学前沿》

2020

聚酰胺纯化膜苞鸢尾中总黄酮的工艺研究

杨阳，张小杰，高文运，赵长琦

《当代化工》

DOI

2020

唾液酸水平对癌症诊断与治疗的作用及其检测方法

杨阳，朱美旗，高文运

《化学与生物工程》

2020

HPLC法检测几种α-酮酸化合物的柱前衍生化条件优化

杨阳，焦淑玲，朱美旗，张曼瑞，高文运

《当代化工》

DOI

2020

HPLC法检测晚期糖化产物前体物质的柱前衍生化条件优化

杨阳，严敏，朱美旗，高文运

《化学工程师》

DOI

2020

α-酮酸检测方法的研究进展

杨阳，刘雪懿，焦淑玲，高文运

《化学工程师》

2020

Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones

Yan‐Fei Liang, Le-Tian Yan, Yue Qiao, Ji-Kui Zhao, Cai-Yun Luo, Feng Gao, Heng Li, Wen‐Yun Gao

Scientific Reports

DOI

2020

Carbon–Carbon Bond Formation or Cleavage

Nan Liu, Xian Hui, Heng Li, Wen‐Yun Gao

DOI

2019

Measurement of α-dicarbonyl compounds in human saliva by pre-column derivatization HPLC

Xin-Jie Wang, Hongxia Zhang, Heng Li, Ai-Hua Zhu, Wen‐Yun Gao

Clinical Chemistry and Laboratory Medicine (CCLM)

DOI

2019

Inhibitory Activity of Plant Essential Oils against E. coli 1-Deoxy-d-xylulose-5-phosphate reductoisomerase

Ge Yan, Bo-Rong Zhu, Fang-Lin Tian, Xian Hui, Heng Li, Yiming Li, Wen‐Yun Gao

Molecules

DOI

2019

Determination of the Activity of 1-Deoxy-d-Xylulose 5-Phosphate Synthase by Pre-column Derivatization-HPLC Using 1,2-Diamino-4,5-Methylenedioxybenzene as a Derivatizing Reagent

Yan‐Fei Liang, Hui Liu, Heng Li, Wen‐Yun Gao

The Protein Journal

DOI

2018

Elevated levels of α-dicarbonyl compounds in the plasma of type II diabetics and their relevance with diabetic nephropathy

Xinjie Wang, Sheng‐Bo Ma, Zhuo-Feng Liu, Heng Li, Wen‐Yun Gao

Journal of Chromatography B

DOI

2018

Quantitative analyses of α-dicarbonyl compounds in food samples by HPLC using 4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine as a derivatizing reagent

Xin-Jie Wang, Feng Gao, Luchao Li, Xian Hui, Heng Li, Wen‐Yun Gao

Microchemical Journal

DOI

2017

3,3′-二氨基联苯胺柱前衍生高效液相色谱法测定酒中双乙酰

王昕洁，王继宇，李恒，惠先，高文运

《色谱》

2017

Antimicrobial mechanism of epigallocatechin gallate and gallocatechin gallate: They target 1-deoxy-d-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway

Xian Hui, Shui-Hong Hua, Qianqian Wu, Heng Li, Wen‐Yun Gao

Archives of Biochemistry and Biophysics

DOI

2017

An improved enzymatic method for the preparation of (R)-phenylacetyl carbinol

Heng Li, Nan Liu, Xian Hui, Wen‐Yun Gao

RSC Advances

DOI

2017

Determination of diacetyl in liquors by high performance liquid chromatography coupled with precolumn derivatization using 3,3'-diaminobenzidine

Xinjie Wang, Jiyu Wang, Heng Li, Xian Hui, Wen‐Yun Gao

Chinese Journal of Chromatography

DOI

2016

Antimicrobial mechanism of theaflavins: They target 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway

Xian Hui, Yue Qiao, Dandan Zhang, Heng Li, Shaoqing Yang, Wen‐Yun Gao

Scientific Reports

DOI

2016

Inhibition of green tea and the catechins against 1-deoxy-d-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway

Xian Hui, Hui Liu, Fang-Lin Tian, Feifei Li, Heng Li, Wen‐Yun Gao

Fitoterapia

DOI

2015

Cloning and characterization of GST fusion tag stabilized large subunit of Escherichia coli acetohydroxyacid synthase I

Heng Li, Nan Liu, Wenting Wang, Ji‐Yu Wang, Wen‐Yun Gao

Journal of Bioscience and Bioengineering

DOI

2015

A Specific Process to Purify 2-Methyl-D-Erythritol-4-Phosphate Enzymatically Converted from D-Glyceraldehyde-3-Phosphate and Pyruvate

Shaoqing Yang, Jian Min Deng, Qianqian Wu, Heng Li, Wen‐Yun Gao

Natural Product Communications

DOI

2013

Mechanistic insights into 1‐deoxy‐ d ‐xylulose 5‐phosphate reductoisomerase, a key enzyme of the MEP terpenoid biosynthetic pathway

Heng Li, Jie Tian, Wei Lin Sun, Wei Qin, Wen‐Yun Gao

FEBS Journal

DOI

2012

2,4-二硝基苯肼柱前衍生高效液相色谱法测定1-脱氧-D-木酮糖-5-磷酸合酶稳态动力学参数

胡玥，王雪娇，李恒，高文运

《分析化学》

2012

几种非环单萜衍生物的制备及表征

王辉，高文运

《化学研究》

2012

Determination of Steady-State Kinetic Parameters of 1-Deoxy-D-xylulose-5-phosphate Synthase by Pre-column Derivatization High Performance Liquid Chromatography Using 2,4-Dinitrophenylhydrazine as Derivative Reagent

Yue Hu, Xuejiao Wang, Li Heng, Wen‐Yun Gao

Chinese Journal of Analytical Chemistry

DOI

2012

ChemInform Abstract: Preparation of Isotope Labeled/Unlabeled Key Intermediates in 2‐Methyl‐D‐erythritol 4‐Phosphate Terpenoid Biosynthetic Pathway

Heng Li, Shaobo Dai, Wen‐Yun Gao

ChemInform

DOI

2012

A chemo-enzymatic cascade for the one-pot synthesis of 1-deoxy-d-xylulose 5-phosphate and 1-deoxy-d-xylulose

Shaobo Dai, Juan Liao, Jie Tian, Heng Li, Wen‐Yun Gao

Tetrahedron Letters

DOI

2012

C–C Bond Formation and Decarboxylation

Alessandra Bonamore, Alberto Macone, Wolfgang Kroutil, Birgit Seisser, Tijs M. Lammens, Daniela De Biase, Maurice C. R. Franssen, Elinor L. Scott, Johan P. M. Sanders, Kenji Miyamoto, Robert Kourist, Shosuke Yoshida, Hiromichi Ohta, Jie Tian, Heng Li, Wen‐Yun Gao, Saravanakumar Shanmuganathan, Dessy Natalia, Lasse Greiner, Pablo Domı́nguez de Marı́a, Yan‐Hong He, Zhi Guan, Jianfeng Cai

DOI

2012

Preparation of Isotope Labeled/Unlabeled Key Intermediates in 2‐Methyl‐ D ‐erythritol 4‐Phosphate Terpenoid Biosynthetic Pathway

Heng Li, Shaobo Dai, Wen‐Yun Gao

Helvetica Chimica Acta

DOI

2012

Preparation and characterization of several acyclic monoterpene derivatives

Wen‐Yun Gao

Chemical Research

2011

优化分光光度法用于玄参中总环烯醚萜类成分的含量测定

张雪梅，王瑞，吴喜民，王新宏，高文运，李医明

《中成药》

2010

同位素标记及未标记的1-脱氧-D-木酮糖-5-磷酸和2-甲基-D-赤藓糖醇-4-磷酸的合成方法

高文运

《有机化学》

2010

An Improved Preparation of D ‐Glyceraldehyde 3‐Phosphate and Its Use in the Synthesis of 1‐Deoxy‐ D ‐xylulose 5‐Phosphate

Heng Li, Jie Tian, Hui Wang, Shao‐Qing Yang, Wen‐Yun Gao

Helvetica Chimica Acta

DOI

2010

Biosynthesis of the iridoid glucoside, lamalbid, in Lamium barbatum

Heng Li, Shaoqing Yang, Hui Wang, Jie Tian, Wen‐Yun Gao

Phytochemistry

DOI

2010

Optimized enzymatic preparation of 1-deoxy-d-xylulose 5-phosphate

Yuefei Zhou, Zhe Cui, Heng Li, Jie Tian, Wen‐Yun Gao

Bioorganic Chemistry

DOI

2000

A new alkaloid and arbutin from the whole plant of Huperzia serrata

高文运，王保德，李医明，蒋山好，朱大元

Chinese Journal of Chemistry

1999

牧马豆生物碱成分研究

高文运，李医明，蒋山好，朱大元

《天然产物研究与开发》

1999

野决明属植物化学研究进展

高文运，朱大元

《天然产物研究与开发》

1999

番红花侧芽中的新蒽醌化合物

高文运，李医明，朱大元

Journal of Integrative Plant Biology

1998

石杉碱庚的结构鉴定

王保德，蒋山好，高文运，朱大元，孔祥铭，杨一青

Journal of Integrative Plant Biology

1997

祁州漏芦亲脂性化学成分的研究

韦汉勋，高文运，关玉昆，黄明画，程东亮，魏立山，田义杰

《兰州大学学报(自然科学版)》

精选作品

期刊论文

The regulatory subunit of acetohydroxyacid synthase enhances the catalysis of its catalytic subunit on 2-ketobutyrate

Catalytic insights of Bacillus subtilis acetolactate decarboxylase

Catalytic insights of acetolactate synthases from different bacteria

Impact of the key residues of 1-Deoxy-D-Xylulose-5-Phosphate synthase on its catalysis

Changes in α-Dicarbonyl Compound Contents during Storage of Various Fruits and Juices

Determination of α-Dicarbonyl compounds in traditional Chinese herbal medicines

Re-investigation of in vitro activity of acetohydroxyacid synthase I holoenzyme from Escherichia coli

The Catalysis of Bacillus subtilis Acetolactate Synthase Is Fundamentally Underestimated

Overall analyses of the reactions catalyzed by acetohydroxyacid synthase/acetolactate synthase using a precolumn derivatization-HPLC method

Characterization of acetolactate decarboxylase of Streptococcus thermophilus and its stereoselectivity in decarboxylation of α-hydroxy-β-ketoacids

Inhibitory Activity of Proanthocyanidins Against Escherichia coli 1-Deoxy-D-Xylulose-5-Phosphate Reductoisomerase

The chemical mechanisms of the enzymes in the branched-chain amino acids biosynthetic pathway and their applications

HPLC法同时测定膜苞鸢尾中两种有效成分

唾液酸检测方法研究进展

HPLC法同时测定中亚鸢尾中3种抗炎活性成分的含量

聚酰胺纯化膜苞鸢尾中总黄酮的工艺研究

唾液酸水平对癌症诊断与治疗的作用及其检测方法

HPLC法检测几种α-酮酸化合物的柱前衍生化条件优化

HPLC法检测晚期糖化产物前体物质的柱前衍生化条件优化

α-酮酸检测方法的研究进展

Preparation of a whole cell catalyst overexpressing acetohydroxyacid synthase of Thermotoga maritima and its application in the syntheses of α-hydroxyketones

Carbon–Carbon Bond Formation or Cleavage

Measurement of α-dicarbonyl compounds in human saliva by pre-column derivatization HPLC

Inhibitory Activity of Plant Essential Oils against E. coli 1-Deoxy-d-xylulose-5-phosphate reductoisomerase

Determination of the Activity of 1-Deoxy-d-Xylulose 5-Phosphate Synthase by Pre-column Derivatization-HPLC Using 1,2-Diamino-4,5-Methylenedioxybenzene as a Derivatizing Reagent

Elevated levels of α-dicarbonyl compounds in the plasma of type II diabetics and their relevance with diabetic nephropathy

Quantitative analyses of α-dicarbonyl compounds in food samples by HPLC using 4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine as a derivatizing reagent

3,3′-二氨基联苯胺柱前衍生高效液相色谱法测定酒中双乙酰

Antimicrobial mechanism of epigallocatechin gallate and gallocatechin gallate: They target 1-deoxy-d-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway

An improved enzymatic method for the preparation of (R)-phenylacetyl carbinol

Determination of diacetyl in liquors by high performance liquid chromatography coupled with precolumn derivatization using 3,3'-diaminobenzidine

Antimicrobial mechanism of theaflavins: They target 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway

Inhibition of green tea and the catechins against 1-deoxy-d-xylulose 5-phosphate reductoisomerase, the key enzyme of the MEP terpenoid biosynthetic pathway

Cloning and characterization of GST fusion tag stabilized large subunit of Escherichia coli acetohydroxyacid synthase I

A Specific Process to Purify 2-Methyl-D-Erythritol-4-Phosphate Enzymatically Converted from D-Glyceraldehyde-3-Phosphate and Pyruvate

Mechanistic insights into 1‐deoxy‐ d ‐xylulose 5‐phosphate reductoisomerase, a key enzyme of the MEP terpenoid biosynthetic pathway

2,4-二硝基苯肼柱前衍生高效液相色谱法测定1-脱氧-D-木酮糖-5-磷酸合酶稳态动力学参数

几种非环单萜衍生物的制备及表征

Determination of Steady-State Kinetic Parameters of 1-Deoxy-D-xylulose-5-phosphate Synthase by Pre-column Derivatization High Performance Liquid Chromatography Using 2,4-Dinitrophenylhydrazine as Derivative Reagent

ChemInform Abstract: Preparation of Isotope Labeled/Unlabeled Key Intermediates in 2‐Methyl‐D‐erythritol 4‐Phosphate Terpenoid Biosynthetic Pathway

A chemo-enzymatic cascade for the one-pot synthesis of 1-deoxy-d-xylulose 5-phosphate and 1-deoxy-d-xylulose

C–C Bond Formation and Decarboxylation

Preparation of Isotope Labeled/Unlabeled Key Intermediates in 2‐Methyl‐ D ‐erythritol 4‐Phosphate Terpenoid Biosynthetic Pathway

Preparation and characterization of several acyclic monoterpene derivatives

优化分光光度法用于玄参中总环烯醚萜类成分的含量测定

同位素标记及未标记的1-脱氧-D-木酮糖-5-磷酸和2-甲基-D-赤藓糖醇-4-磷酸的合成方法

An Improved Preparation of D ‐Glyceraldehyde 3‐Phosphate and Its Use in the Synthesis of 1‐Deoxy‐ D ‐xylulose 5‐Phosphate

Biosynthesis of the iridoid glucoside, lamalbid, in Lamium barbatum

Optimized enzymatic preparation of 1-deoxy-d-xylulose 5-phosphate

A new alkaloid and arbutin from the whole plant of Huperzia serrata

牧马豆生物碱成分研究

野决明属植物化学研究进展

番红花侧芽中的新蒽醌化合物

石杉碱庚的结构鉴定

祁州漏芦亲脂性化学成分的研究

会议论文

萜类化合物生物合成的MEP途径中关键酶的作用机制

高效液相法检测DXP、D-GAP及DHAP的方法

一种从植物提取物中筛选1-脱氧-D-木酮糖5-磷酸还原异构化酶抑制剂的简单方法

萜类化合物生物合成的MEP途径中中间体转化机理的研究

萜类化合物MEP生物合成途径中关键酶DXR的合成及功能鉴定

萜类化合物MEP生物合成途径中DXS酶的制备和功能鉴定

书籍章节

1-Deoxy-d-xylulose 5-phosphate reductoisomerase, the first committed enzyme in the MEP terpenoid biosynthetic pathway—Its chemical mechanism and inhibition

研究方向