付新苗｜福州大学｜学者主页

2026

Genome‐wide screen reveals a universal role of ATP in ciprofloxacin tolerance among genetically distinct Escherichia coli persisters

Zhenfang Mei, Ya-Wen-Cai-Ya-Wen Cai, Jianfeng Huang, Ke-Dong Zhao, Zuqin Zhang, Dan-Dan Yu, Shiyan Lu, Zeying Lai, T. K. Wood, Xinmiao Fu

mLife

DOI

2026

Disruption of Periplasmic Chaperone-OmpF Interaction as an Efficient Antibacterial Strategy against Gram-Negative Bacteria.

Yan Wang, Shiyan Lu, Shuting Shi, Xin Jiang, Danyan Chen, Shuang Zhang, Yu-Chan Wang, Bo-Yan Lv, Yu Cheng, Ke Li, Ping Gao, Xue-Ping Yu, Xinmiao Fu

ACS Infectious Diseases

DOI

2026

Risk factors for antibiotic resistance and 30-day mortality among adult patients with acute leukemia and Escherichia coli bloodstream infection.

Juan Wu, Wei Wang, Kaili Du, Zhongxian Liao, Yawei Shi, Munire Abudumaijiti, Ji-Lai Liu, Jia-Di Chen, Xinmiao Fu

European Journal of Clinical Microbiology and Infectious Diseases

DOI

2025

Sonoporation-enhanced the antibacterial efficacy of aminoglycosides against Gram-negative persisters

Hongyi Chen, Jing-Kai Wang, Fang Han, Chen Guo, Xinmiao Fu, Zhiqiang Zhang, Yizhi Song

World Journal of Microbiology & Biotechnology

DOI

2025

Genome-wide screen reveals glycerol-induced aminoglycoside potentiation against Staphylococcus aureus via boosting GlpK-initiated energy metabolism

Ya-Qin Xing, Mengmeng Bian, Xuebing Huang, Bo-Yan Lv, Zhijie Huang, Xian-Zhang Jiang, Weiya Huang, Huping Xue, Hangyu Zhao, Jianfeng Huang, Xinmiao Fu

Antimicrobial Agents and Chemotherapy

DOI

2025

Rationally Designed Self-Derived Peptides Kill Escherichia coli by Targeting BamA and BamD Essential for Outer Membrane Protein Biogenesis.

Yu-Chan Wang, Yu Cheng, Ying-Hong Li, Yan Wang, Xinmiao Fu

ACS Infectious Diseases

DOI

2025

Risk factors and outcomes of antibiotic-resistant Klebsiella pneumoniae bloodstream infections in adult patients with acute leukemia

Juan Wu, Zhiqiang Xie, Xue-Yong Li, Kaili Du, Zhongxian Liao, Yawei Shi, Munire Abudumaijiti, Jia-Di Chen, Ying-Ping Cao, Xinmiao Fu

BMC Microbiology

DOI

2023

Heat shock potentiates aminoglycosides against gram-negative bacteria by enhancing antibiotic uptake, protein aggregation, and ROS

Bo-Yan Lv, Xue-Bing Huang, Chenchen Lijia, Yue-Long Ma, Mengmeng Bian, Zhongyan Li, J. Duan, Fang Zhou, Bin Yang, Xingwang Qie, Yi-Zhi Song, T. Wood, Xinmiao Fu

Proceedings of the National Academy of Sciences of the United States of America

DOI

2023

CCCP Facilitates Aminoglycoside to Kill Late Stationary-Phase Escherichia coli by Elevating Hydroxyl Radical.

Zhongyan Li, Linchao Wu, Zhijie Huang, Bo-Yan Lv, Yajuan Fu, Lun-Jiang Zhou, Xinmiao Fu

ACS Infectious Diseases

DOI

2022

5-Methylindole kills various bacterial pathogens and potentiates aminoglycoside against methicillin-resistant Staphylococcus aureus

Zhongyan Li, Feng-Qi Sun, Xinmiao Fu, Ya-Juan Chen

PeerJ

DOI

2022

n-Butanol Potentiates Subinhibitory Aminoglycosides against Bacterial Persisters and Multidrug-Resistant MRSA by Rapidly Enhancing Antibiotic Uptake.

Bo-Yan Lv, Mengmeng Bian, Xue-Bing Huang, Feng-Qi Sun, Yuanyuan Gao, Yan Wang, Yajuan Fu, Bining Yang, Xinmiao Fu

ACS Infectious Diseases

DOI

2021

Gentamicin Combined With Hypoionic Shock Rapidly Eradicates Aquaculture Bacteria in vitro and in vivo

Yuanyuan Gao, Zhong-miao Chen, Wei Yao, Daliang Li, Xinmiao Fu

Frontiers in Microbiology

DOI

2021

Mechanosensitive Channels Mediate Hypoionic Shock-Induced Aminoglycoside Potentiation against Bacterial Persisters by Enhancing Antibiotic Uptake

Bo-Yan Lv, Youhui Zeng, Huai-Dong Zhang, Zhongyan Li, Zhaorong Xu, Yan Wang, Yuanyuan Gao, Ya-Juan Chen, Xinmiao Fu

Antimicrobial Agents and Chemotherapy

DOI

2020

Rapid Freezing Enables Aminoglycosides To Eradicate Bacterial Persisters via Enhancing Mechanosensitive Channel MscL-Mediated Antibiotic Uptake

Yan-Na Zhao, Bo-Yan Lv, Feng-Qi Sun, Jia-Feng Liu, Yan Wang, Yuanyuan Gao, Feng Qi, Z. Chang, Xinmiao Fu

mBio

DOI

2020

5-Methylindole Potentiates Aminoglycoside Against Gram-Positive Bacteria Including Staphylococcus aureus Persisters Under Hypoionic Conditions

Feng-Qi Sun, Mengmeng Bian, Zhongyan Li, Bo-Yan Lv, Yuanyuan Gao, Yan Wang, Xinmiao Fu

Frontiers in Cellular and Infection Microbiology

DOI

2019

Hypoionic Shock Facilitates Aminoglycoside Killing of Both Nutrient Shift- and Starvation-Induced Bacterial Persister Cells by Rapidly Enhancing Aminoglycoside Uptake

Zhong-miao Chen, Yuanyuan Gao, Bo-Yan Lv, Feng-Qi Sun, Wei Yao, Yan Wang, Xinmiao Fu

Frontiers in Microbiology

DOI

2019

DegP degrades a wide range of substrate proteins in Escherichia coli under stress conditions.

Shuang Zhang, Yu Cheng, Jing Ma, Yan Wang, Z. Chang, Xinmiao Fu

Biochemical Journal

DOI

2018

DegP functions as a critical protease for bacterial acid resistance

Xinmiao Fu, Yan Wang, Heqi Shao, Jing Ma, Xin-Wen Song, Meng Zhang, Z. Chang

The FEBS Journal

DOI

2016

ATP合酶旋转催化的一种新机制

刘佳峰，付新苗，昌增益

《中国科学:生命科学》

2016

A Supercomplex Spanning the Inner and Outer Membranes Mediates the Biogenesis of β-Barrel Outer Membrane Proteins in Bacteria*

Yan Wang, Rui Wang, Feng Jin, Yang Liu, Jia-Yu Yu, Xinmiao Fu, Z. Chang

Journal of Biological Chemistry

DOI

2015

A reciprocating motion-driven rotation mechanism for the ATP synthase

Jia-Feng Liu, Xinmiao Fu, Z. Chang

Science China Life Sciences

DOI

2015

A Novel Mechanism for Small Heat Shock Proteins to Function as Molecular Chaperones

Kaiming Zhang, Anastasia N Ezemaduka, Zhao Wang, Hong-Li Hu, Xiao-Dong Shi, Chuang Liu, Xin-Ping Lu, Xinmiao Fu, Z. Chang, Chang-Cheng Yin

Scientific Reports

DOI

2015

Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters

Jia-Feng Liu, Xinmiao Fu, Z. Chang

Scientific Reports

DOI

2015

Insights into How Small Heat Shock Proteins Bind a Great Diversity of Substrate Proteins: A Super-Transformer Model

Xinmiao Fu

DOI

2014

Multilevel structural characteristics for the natural substrate proteins of bacterial small heat shock proteins

Xinmiao Fu, Z. Chang, Xiao-Dong Shi, Dong-Bo Bu, Chao Wang

Protein Science

DOI

2014

Differential degradation for small heat shock proteins IbpA and IbpB is synchronized in Escherichia coli: implications for their functional cooperation in substrate refolding.

Xiao-Dong Shi, Linxuan Yan, Han-Lin Zhang, K. Sun, Z. Chang, Xinmiao Fu

Biochemical and Biophysical Research Communications - BBRC

DOI

2014

A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50°C Conceivably by Maintaining Cell Envelope Integrity

Anastasia N Ezemaduka, Jia-Yu Yu, Xiao-Dong Shi, Kaiming Zhang, Chang-Cheng Yin, Xinmiao Fu, Z. Chang

Journal of Bacteriology

DOI

2014

Chaperone function and mechanism of small heat-shock proteins.

Xinmiao Fu

Acta Biochimica et Biophysica Sinica

DOI

2014

DegP primarily functions as a protease for the biogenesis of β‐barrel outer membrane proteins in the Gram‐negative bacterium Escherichia coli

Xi Ge, Rui Wang, Jing Ma, Yang Liu, Anastasia N. Ezemaduka, Peng R. Chen, Xinmiao Fu, Z. Chang

The FEBS Journal

DOI

2013

Small Heat Shock Protein IbpB Acts as a Robust Chaperone in Living Cells by Hierarchically Activating Its Multi-type Substrate-binding Residues*

Xinmiao Fu, Xiao-Dong Shi, L. Yin, Jia-Feng Liu, K. Joo, Jooyoung Lee, Z. Chang

Journal of Biological Chemistry

DOI

2013

In Vivo Substrate Diversity and Preference of Small Heat Shock Protein IbpB as Revealed by Using a Genetically Incorporated Photo-cross-linker*

Xinmiao Fu, Xiao-Dong Shi, Linxuan Yan, Han-Lin Zhang, Z. Chang

Journal of Biological Chemistry

DOI

2013

the biogenesis of outer membrane proteins under heat

Xi Ge, Zhi-Xin Lyu, Yang Liu, Rui Wang, Xin-Sheng Zhao, Xinmiao Fu, Z. Chang

2013

Identification of FkpA as a Key Quality Control Factor for the Biogenesis of Outer Membrane Proteins under Heat Shock Conditions

Xi Ge, Zhi-Xin Lyu, Yang Liu, Rui Wang, Xin-Sheng Zhao, Xinmiao Fu, Z. Chang

Journal of Bacteriology

DOI

2012

Chaperone-dependent mechanisms for acid resistance in enteric bacteria.

Weizhe Hong, Y. E. Wu, Xinmiao Fu, Z. Chang

Trends in Microbiology

DOI

2011

A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.

Meng Zhang, Shi-Xian Lin, Xin-Wen Song, Jun Liu, Ye Fu, Xi Ge, Xinmiao Fu, Z. Chang, Peng R. Chen

Nature Chemical Biology

DOI

2011

Small heat shock protein AgsA forms dynamic fibrils

Xiao-Dong Shi, Zhao Wang, Linxuan Yan, Anastasia N. Ezemaduka, G. Fan, Rui Wang, Xinmiao Fu, Chang-Cheng Yin, Z. Chang

FEBS Letters

DOI

2006

Phylogenetic and Biochemical Studies Reveal a Potential Evolutionary Origin of Small Heat Shock Proteins of Animals from Bacterial Class A

Xinmiao Fu, Wangwang Jiao, Z. Chang

Journal of Molecular Evolution

DOI

2006

Identification of a highly conserved pro-gly doublet in non-animal small heat shock proteins and characterization of its structural and functional roles in Mycobacterium tuberculosis Hsp 16.3

Xinmiao Fu, Z. Chang

Biochemistry (Moscow)

DOI

2006

Identification of bis-ANS binding sites in Mycobacterium tuberculosis small heat shock protein Hsp16.3: evidences for a two-step substrate-binding mechanism.

Xinmiao Fu, Z. Chang

Biochemical and Biophysical Research Communications - BBRC

DOI

2005

A Dual Role for the N-terminal Region of Mycobacterium tuberculosis Hsp16.3 in Self-oligomerization and Binding Denaturing Substrate Proteins*

Xinmiao Fu, Hui Zhang, Xue-Feng Zhang, Yang Cao, Wangwang Jiao, Chong Liu, Yang Song, A. Abulimiti, Z. Chang

Journal of Biological Chemistry

DOI

2005

Chaperone-Like Activity of Mycobacterium tuberculosis Hsp16.3 Does Not Require Its Intact (Native) Structures

Xiao-You Chen, Xinmiao Fu, Yu Ma, Z. Chang

Biochemistry (Moscow)

DOI

2005

The association of small heat shock protein Hsp16.3 with the plasma membrane of Mycobacterium tuberculosis: dissociation of oligomers is a prerequisite.

Hui Zhang, Xinmiao Fu, Wangwang Jiao, Xue-Feng Zhang, Chong Liu, Z. Chang

Biochemical and Biophysical Research Communications - BBRC

DOI

2005

Periplasmic Protein HdeA Exhibits Chaperone-like Activity Exclusively within Stomach pH Range by Transforming into Disordered Conformation*

Weizhe Hong, Wangwang Jiao, Jicheng Hu, Junrui Zhang, Chong Liu, Xinmiao Fu, D. Shen, B. Xia, Z. Chang

Journal of Biological Chemistry

DOI

2004

结核杆菌小分子热休克蛋白Hsp16.3调控分子伴侣活性的结构基础

付新苗

《清华大学》

2004

Periplasmic proteins of Escherichia coli are highly resistant to aggregation: reappraisal for roles of molecular chaperones in periplasm.

Yang Liu, Xinmiao Fu, Jia-Liang Shen, Hui Zhang, Weizhe Hong, Z. Chang

Biochemical and Biophysical Research Communications - BBRC

DOI

2004

Inter-subunit Cross-linking Suppressed the Dynamic Oligomeric Dissociation of Mycobacterium tuberculosis Hsp16.3 and Reduced Its Chaperone Activity

Xinmiao Fu, Wangwang Jiao, A. Abulimiti, Z. Chang

Biochemistry (Moscow)

DOI

2004

Temperature-dependent subunit exchange and chaperone-like activities of Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis.

Xinmiao Fu, Z. Chang

Biochemical and Biophysical Research Communications - BBRC

DOI

2003

Small heat shock protein Hsp16.3 modulates its chaperone activity by adjusting the rate of oligomeric dissociation.

Xinmiao Fu, Chong Liu, Yang Liu, Xiu-Guang Feng, Liang-Cai Gu, Xiao-You Chen, Z. Chang

Biochemical and Biophysical Research Communications - BBRC

DOI

2003

Mycobacterium tuberculosis Hsp16.3 nonamers are assembled and re-assembled via trimer and hexamer intermediates.

A. Abulimiti, Xinmiao Fu, Liang-Cai Gu, Xiu-Guang Feng, Z. Chang

Journal of Molecular Biology

DOI

2003

Disulfide bonds convert small heat shock protein Hsp16.3 from a chaperone to a non-chaperone: implications for the evolution of cysteine in molecular chaperones.

Xinmiao Fu, Wen Li, Q. Mao, Z. Chang

Biochemical and Biophysical Research Communications - BBRC

DOI

2002

The reassembling process of the nonameric Mycobacterium tuberculosis small heat-shock protein Hsp16.3 occurs via a stepwise mechanism.

Xiu-Guang Feng, Su-Fang Huang, Xinmiao Fu, A. Abulimiti, Z. Chang

Biochemical Journal

DOI

精选作品

期刊论文

Genome‐wide screen reveals a universal role of ATP in ciprofloxacin tolerance among genetically distinct Escherichia coli persisters

Disruption of Periplasmic Chaperone-OmpF Interaction as an Efficient Antibacterial Strategy against Gram-Negative Bacteria.

Risk factors for antibiotic resistance and 30-day mortality among adult patients with acute leukemia and Escherichia coli bloodstream infection.

Sonoporation-enhanced the antibacterial efficacy of aminoglycosides against Gram-negative persisters

Genome-wide screen reveals glycerol-induced aminoglycoside potentiation against Staphylococcus aureus via boosting GlpK-initiated energy metabolism

Rationally Designed Self-Derived Peptides Kill Escherichia coli by Targeting BamA and BamD Essential for Outer Membrane Protein Biogenesis.

Risk factors and outcomes of antibiotic-resistant Klebsiella pneumoniae bloodstream infections in adult patients with acute leukemia

Heat shock potentiates aminoglycosides against gram-negative bacteria by enhancing antibiotic uptake, protein aggregation, and ROS

CCCP Facilitates Aminoglycoside to Kill Late Stationary-Phase Escherichia coli by Elevating Hydroxyl Radical.

5-Methylindole kills various bacterial pathogens and potentiates aminoglycoside against methicillin-resistant Staphylococcus aureus

n-Butanol Potentiates Subinhibitory Aminoglycosides against Bacterial Persisters and Multidrug-Resistant MRSA by Rapidly Enhancing Antibiotic Uptake.

Gentamicin Combined With Hypoionic Shock Rapidly Eradicates Aquaculture Bacteria in vitro and in vivo

Mechanosensitive Channels Mediate Hypoionic Shock-Induced Aminoglycoside Potentiation against Bacterial Persisters by Enhancing Antibiotic Uptake

Rapid Freezing Enables Aminoglycosides To Eradicate Bacterial Persisters via Enhancing Mechanosensitive Channel MscL-Mediated Antibiotic Uptake

5-Methylindole Potentiates Aminoglycoside Against Gram-Positive Bacteria Including Staphylococcus aureus Persisters Under Hypoionic Conditions

Hypoionic Shock Facilitates Aminoglycoside Killing of Both Nutrient Shift- and Starvation-Induced Bacterial Persister Cells by Rapidly Enhancing Aminoglycoside Uptake

DegP degrades a wide range of substrate proteins in Escherichia coli under stress conditions.

DegP functions as a critical protease for bacterial acid resistance

ATP合酶旋转催化的一种新机制

A Supercomplex Spanning the Inner and Outer Membranes Mediates the Biogenesis of β-Barrel Outer Membrane Proteins in Bacteria*

A reciprocating motion-driven rotation mechanism for the ATP synthase

A Novel Mechanism for Small Heat Shock Proteins to Function as Molecular Chaperones

Hypoionic shock treatment enables aminoglycosides antibiotics to eradicate bacterial persisters

Insights into How Small Heat Shock Proteins Bind a Great Diversity of Substrate Proteins: A Super-Transformer Model

Multilevel structural characteristics for the natural substrate proteins of bacterial small heat shock proteins

Differential degradation for small heat shock proteins IbpA and IbpB is synchronized in Escherichia coli: implications for their functional cooperation in substrate refolding.

A Small Heat Shock Protein Enables Escherichia coli To Grow at a Lethal Temperature of 50°C Conceivably by Maintaining Cell Envelope Integrity

Chaperone function and mechanism of small heat-shock proteins.

DegP primarily functions as a protease for the biogenesis of β‐barrel outer membrane proteins in the Gram‐negative bacterium Escherichia coli

Small Heat Shock Protein IbpB Acts as a Robust Chaperone in Living Cells by Hierarchically Activating Its Multi-type Substrate-binding Residues*

In Vivo Substrate Diversity and Preference of Small Heat Shock Protein IbpB as Revealed by Using a Genetically Incorporated Photo-cross-linker*

the biogenesis of outer membrane proteins under heat

Identification of FkpA as a Key Quality Control Factor for the Biogenesis of Outer Membrane Proteins under Heat Shock Conditions

Chaperone-dependent mechanisms for acid resistance in enteric bacteria.

A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.

Small heat shock protein AgsA forms dynamic fibrils

Phylogenetic and Biochemical Studies Reveal a Potential Evolutionary Origin of Small Heat Shock Proteins of Animals from Bacterial Class A

Identification of a highly conserved pro-gly doublet in non-animal small heat shock proteins and characterization of its structural and functional roles in Mycobacterium tuberculosis Hsp 16.3

Identification of bis-ANS binding sites in Mycobacterium tuberculosis small heat shock protein Hsp16.3: evidences for a two-step substrate-binding mechanism.

A Dual Role for the N-terminal Region of Mycobacterium tuberculosis Hsp16.3 in Self-oligomerization and Binding Denaturing Substrate Proteins*

Chaperone-Like Activity of Mycobacterium tuberculosis Hsp16.3 Does Not Require Its Intact (Native) Structures

The association of small heat shock protein Hsp16.3 with the plasma membrane of Mycobacterium tuberculosis: dissociation of oligomers is a prerequisite.

Periplasmic Protein HdeA Exhibits Chaperone-like Activity Exclusively within Stomach pH Range by Transforming into Disordered Conformation*

结核杆菌小分子热休克蛋白Hsp16.3调控分子伴侣活性的结构基础

Periplasmic proteins of Escherichia coli are highly resistant to aggregation: reappraisal for roles of molecular chaperones in periplasm.

Inter-subunit Cross-linking Suppressed the Dynamic Oligomeric Dissociation of Mycobacterium tuberculosis Hsp16.3 and Reduced Its Chaperone Activity

Temperature-dependent subunit exchange and chaperone-like activities of Hsp16.3, a small heat shock protein from Mycobacterium tuberculosis.

Small heat shock protein Hsp16.3 modulates its chaperone activity by adjusting the rate of oligomeric dissociation.

Mycobacterium tuberculosis Hsp16.3 nonamers are assembled and re-assembled via trimer and hexamer intermediates.

Disulfide bonds convert small heat shock protein Hsp16.3 from a chaperone to a non-chaperone: implications for the evolution of cysteine in molecular chaperones.

The reassembling process of the nonameric Mycobacterium tuberculosis small heat-shock protein Hsp16.3 occurs via a stepwise mechanism.

会议论文

提高氨基糖苷类抗生素杀菌效率的新方法及其分子机理

The functionally active conformation of the acid resistant molecular chaperone HdeA is disordered

Temperature-dependent contributions of DegP to the quality control of bacterial outer membrane protein biogenesis

Homo-oligomerization as a common way for Proteins to modulate their activities

研究方向