韩文元｜华中农业大学｜学者主页

2026

Recruitment of Cas3 enables DNA cleavage by the type I-A CRISPR-Cas system of Saccharolobus islandicus

Suping Jiang, Xuhui Tian, Fang Wang, Wenyuan Han, Qunxin She, Xu Feng

Cell Reports

2026

A phage-encoded sponge protein suppresses bacterial TIR-Caspase immune signaling

Yu Chen, Linlin Wei, Zhenhao Han, Ruiliang Zhao, Zhifeng Zeng, Yanqiu Liu, Piaoran Zhang, Keyi Tan, Yue Fan, Long Sheng, Hao Wang, Rafael Pinilla‐Redondo, Yue Feng, Wenyuan Han

Nature Communications

DOI

2026

Systematic discovery of bacterial anti-phage systems through a protein domain-centric strategy

Yanqiu Liu, Keyi Tan, Zhenhao Han, Yu Chen, Kangyi Xiao, Pian Luo, RuiPeng Fan, Fuming Liang, Rafael Pinilla‐Redondo, Wenyuan Han

Nucleic Acids Research

DOI

2026

Filament-mediated repurposing of toxic dITP for immunity in the Kongming system

Hao Feng, Kai Shao, Zhifeng Zeng, Eddie Yong Jun Tan, Zeyu Hu, Ruiliang Zhao, Jikai Rao, Jian Shi, Zhuojian Chen, Rafael Pinilla‐Redondo, Bin Wu, Wenyuan Han, Min Luo

Molecular Cell

DOI

2025

An archaeal chronic virus escapes the immunity of prokaryotic Argonaute

Yu Chen, Yanqiu Liu, Keyi Tan, Ke Yang, Zhifeng Zeng, Yu Guo, Piaoran Zhang, Wenyuan Han

Nucleic Acids Research

DOI

2025

Base-modified nucleotides mediate immune signaling in bacteria

Zhifeng Zeng, Zeyu Hu, Ruiliang Zhao, Jikai Rao, Mario Rodríguez Mestre, Yanqiu Liu, Shunhang Liu, Hao Feng, Yu Chen, Huan He, Nuo Chen, Jinshui Zheng, Donghai Peng, Min Luo, Qunxin She, Rafael Pinilla‐Redondo, Wenyuan Han

Science

DOI

2025

Structural and mechanistic insights into the activation of a short prokaryotic argonaute system from archaeon Sulfolobus islandicus

Zhikang Dai, Yu Chen, Zeyuan Guan, Xueting Chen, Keyi Tan, Kaiyue Yang, Xuhui Yan, Yidong Liu, Zhou Gong, Wenyuan Han, Tingting Zou

Nucleic Acids Research

DOI

2025

A mobile genetic element-derived primase-polymerase harbors multiple activities implicated in DNA replication and repair

Pan Fu, Yuwei Wang, Yanqiu Liu, Zhenhao Han, Zhong Peng, Linfeng Liu, Wenyuan Han

Nucleic Acids Research

DOI

2025

Phase variation in Bacteroides fragilis governs susceptibility to a microvirus and drives its evolution

Pan Huang, Meiqi Du, Yanqiu Liu, Zhenhao Han, Qian Wan, Fuming Liang, Wenyuan Han

Engineering Microbiology

DOI

2025

Leveraging defense system modularity to discover anti-phage systems

Yanqiu Liu, Keyi Tan, Zhenhao Han, Yu Chen, Kang-Wen Xiao, P. Luo, RuiPeng Fan, Fuming Liang, R. Pinilla-Redondo, Wenyuan Han

bioRxiv

DOI

2024

Catalytically active prokaryotic Argonautes employ phospholipase D family proteins to strengthen immunity against different genetic invaders

Feiyue Cheng, Aici Wu, Zhihua Li, Jing Liang Xu, Xifeng Cao, Haiying Yu, Zhenquan Liu, Rui Wang, Wenyuan Han, Hua Xiang, Ming Li

mLife

DOI

2024

Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope

Xu Feng, Ruyi Xu, Jianglan Liao, Jingyu Zhao, Baochang Zhang, Xiaoxiao Xu, Pengpeng Zhao, Xiao‐Ning Wang, Jianyun Yao, Pengxia Wang, Xiaoxue Wang, Wenyuan Han, Qunxin She

Nature Communications

DOI

2024

HAMpering infection: Helicase ratcheting emerges as a phage-sensing mechanism

Zhifeng Zeng, Wenyuan Han

Cell Host & Microbe

DOI

2023

Catalytically inactive long prokaryotic Argonaute systems employ distinct effectors to confer immunity via abortive infection

Xinmi Song, Sheng Lei, Shunhang Liu, Yanqiu Liu, Pan Fu, Zhifeng Zeng, Ke Yang, Yu Chen, Ming Li, Qunxin She, Wenyuan Han

Nature Communications

DOI

2022

The abortive infection functions of CRISPR-Cas and Argonaute

Yu Chen, Zhifeng Zeng, Qunxin She, Wenyuan Han

Trends in Microbiology

DOI

2022

Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

Yu Feng Zou, Lu Qiu, Aowen Xie, Wenyuan Han, Shangbo Zhang, Jinshan Li, Shumiao Zhao, Yingjun Li, Yunxiang Liang, Yongmei Hu

Microbial Cell Factories

DOI

2022

Inactivation of Target RNA Cleavage of a III-B CRISPR-Cas System Induces Robust Autoimmunity in Saccharolobus islandicus

Yan Zhang, Jinzhong Lin, Xuhui Tian, Yuan Wang, Ruiliang Zhao, Chenwei Wu, Xiao‐Ning Wang, Pengpeng Zhao, Xiaonan Bi, Zhenxiao Yu, Wenyuan Han, Nan Peng, Yun Xiang Liang, Qunxin She

International Journal of Molecular Sciences

DOI

2022

A short prokaryotic Argonaute activates membrane effector to confer antiviral defense

Zhifeng Zeng, Yu Chen, Rafael Pinilla‐Redondo, Shiraz Ali Shah, Fen Zhao, Chen Wang, Zeyu Hu, Chang Guang Wu, Changyi Zhang, Rachel J. Whitaker, Qunxin She, Wenyuan Han

Cell Host & Microbe

DOI

2022

The trans DNA cleavage activity of Cas12a provides no detectable immunity against plasmid or phage

Shunhang Liu, Xichen Rao, Ruiliang Zhao, Wenyuan Han

Frontiers in Genome Editing

DOI

2021

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

Ruiliang Zhao, Yang Yang, Ke Yang, Wenyuan Han

STAR Protocols

DOI

2020

CRISPR-Associated Factor Csa3b Regulates CRISPR Adaptation and Cmr-Mediated RNA Interference in Sulfolobus islandicus

Qing Ye, Xueqiao Zhao, Jilin Liu, Zhifeng Zeng, Zhufeng Zhang, Tao Liu, Yingjun Li, Wenyuan Han, Nan Peng

Frontiers in Microbiology

DOI

2020

A Unique B-Family DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota

Xu Feng, Xiaotong Liu, Ruyi Xu, Ruiliang Zhao, Wenqian Feng, Jianglan Liao, Wenyuan Han, Qunxin She

Frontiers in Microbiology

DOI

2020

A Membrane-Associated DHH-DHHA1 Nuclease Degrades Type III CRISPR Second Messenger

Ruiliang Zhao, Yang Yang, Fan Zheng, Zhifeng Zeng, Wenqian Feng, Xuexia Jin, Jiayi Wang, Ke Yang, Yun Xiang Liang, Qunxin She, Wenyuan Han

Cell Reports

DOI

2019

Cmr3 regulates the suppression on cyclic oligoadenylate synthesis by tag complementarity in a Type III-B CRISPR-Cas system

Tong Guo, Fan Zheng, Zhifeng Zeng, Yang Yang, Qi Li, Qunxin She, Wenyuan Han

RNA Biology

DOI

2018

A Type III-B Cmr effector complex catalyzes the synthesis of cyclic oligoadenylate second messengers by cooperative substrate binding

Wenyuan Han, Stefano Stella, Yan Zhang, Tong Guo, Karolina Sulek, Li Peng-Lundgren, Guillermo Montoya, Qunxin She

Nucleic Acids Research

DOI

2018

Tolerance of Sulfolobus SMV1 virus to the immunity of I-A and III-B CRISPR-Cas systems in Sulfolobus islandicus

Tong Guo, Wenyuan Han, Qun-Xin She

RNA Biology

DOI

2018

Conserved accessory proteins encoded with archaeal and bacterial Type III CRISPR-Cas gene cassettes that may specifically modulate, complement or extend interference activity

Shiraz A. Shah, O. Alkhnbashi, Juliane Behler, Wenyuan Han, Qun-Xin She, W. Hess, R. Garrett, R. Backofen

bioRxiv

DOI

2018

Comprehensive search for accessory proteins encoded with archaeal and bacterial type III CRISPR-cas gene cassettes reveals 39 new cas gene families

Shiraz A. Shah, O. Alkhnbashi, Juliane Behler, Wenyuan Han, Qun-Xin She, W. Hess, R. Garrett, R. Backofen

RNA Biology

DOI

2017

Allosteric regulation of Csx1, a type IIIB-associated CARF domain ribonuclease by RNAs carrying a tetraadenylate tail

Wenyuan Han, Saifu Pan, B. López-Méndez, G. Montoya, Qun-Xin She

Nucleic Acids Research

DOI

2017

Cmr1 enables efficient RNA and DNA interference of a III-B CRISPR–Cas system by binding to target RNA and crRNA

Ying-Jun Li, Yan Zhang, Jinzhong Lin, Saifu Pan, Wenyuan Han, Nan Peng, Yun-Xiang Liang, Qun-Xin She

Nucleic Acids Research

DOI

2017

CRISPR History: Discovery, Characterization, and Prosperity.

Wenyuan Han, Qun-Xin She

Progress in Molecular Biology and Translational Science

DOI

2017

Archaea and CRISPR biology

Qun-Xin She, Wenyuan Han

2016

A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction

Wenyuan Han, Ying-Jun Li, Ling Deng, Mingxia Feng, Wenfang Peng, S. Hallstrøm, Jing Zhang, Nan Peng, Yun-Xiang Liang, M. White, Qun-Xin She

Nucleic Acids Research

DOI

2012

DNA重组酶同系物stRadC2在超嗜热古菌Sulfolobus tokodaii细胞内的功能分析

王蕾，盛多红，韩文元，黄彬，朱姗姗，倪金风，李佳，申玉龙

《中国科学:生命科学》

精选作品

期刊论文

Recruitment of Cas3 enables DNA cleavage by the type I-A CRISPR-Cas system of Saccharolobus islandicus

A phage-encoded sponge protein suppresses bacterial TIR-Caspase immune signaling

Systematic discovery of bacterial anti-phage systems through a protein domain-centric strategy

Filament-mediated repurposing of toxic dITP for immunity in the Kongming system

An archaeal chronic virus escapes the immunity of prokaryotic Argonaute

Base-modified nucleotides mediate immune signaling in bacteria

Structural and mechanistic insights into the activation of a short prokaryotic argonaute system from archaeon Sulfolobus islandicus

A mobile genetic element-derived primase-polymerase harbors multiple activities implicated in DNA replication and repair

Phase variation in Bacteroides fragilis governs susceptibility to a microvirus and drives its evolution

Leveraging defense system modularity to discover anti-phage systems

Catalytically active prokaryotic Argonautes employ phospholipase D family proteins to strengthen immunity against different genetic invaders

Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope

HAMpering infection: Helicase ratcheting emerges as a phage-sensing mechanism

Catalytically inactive long prokaryotic Argonaute systems employ distinct effectors to confer immunity via abortive infection

The abortive infection functions of CRISPR-Cas and Argonaute

Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

Inactivation of Target RNA Cleavage of a III-B CRISPR-Cas System Induces Robust Autoimmunity in Saccharolobus islandicus

A short prokaryotic Argonaute activates membrane effector to confer antiviral defense

The trans DNA cleavage activity of Cas12a provides no detectable immunity against plasmid or phage

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

CRISPR-Associated Factor Csa3b Regulates CRISPR Adaptation and Cmr-Mediated RNA Interference in Sulfolobus islandicus

A Unique B-Family DNA Polymerase Facilitating Error-Prone DNA Damage Tolerance in Crenarchaeota

A Membrane-Associated DHH-DHHA1 Nuclease Degrades Type III CRISPR Second Messenger

Cmr3 regulates the suppression on cyclic oligoadenylate synthesis by tag complementarity in a Type III-B CRISPR-Cas system

A Type III-B Cmr effector complex catalyzes the synthesis of cyclic oligoadenylate second messengers by cooperative substrate binding

Tolerance of Sulfolobus SMV1 virus to the immunity of I-A and III-B CRISPR-Cas systems in Sulfolobus islandicus

Conserved accessory proteins encoded with archaeal and bacterial Type III CRISPR-Cas gene cassettes that may specifically modulate, complement or extend interference activity

Comprehensive search for accessory proteins encoded with archaeal and bacterial type III CRISPR-cas gene cassettes reveals 39 new cas gene families

Allosteric regulation of Csx1, a type IIIB-associated CARF domain ribonuclease by RNAs carrying a tetraadenylate tail

Cmr1 enables efficient RNA and DNA interference of a III-B CRISPR–Cas system by binding to target RNA and crRNA

CRISPR History: Discovery, Characterization, and Prosperity.

Archaea and CRISPR biology

A type III-B CRISPR-Cas effector complex mediating massive target DNA destruction

DNA重组酶同系物stRadC2在超嗜热古菌Sulfolobus tokodaii细胞内的功能分析

会议论文

硫化叶菌重组酶RadA同源蛋白RadC功能的初步研究

预印本

A Membrane-Associated Nuclease Degrades Cyclic Oligo-Adenylate and Deactivates Type III CRISPR Ribonuclease

研究方向