吴静｜江南大学｜学者主页

2026

构建整合型重组大肠杆菌高效合成软骨素

黄凌馨，孟伟伟，齐禾嘉，宋祥廷，张琳浩，刘立明，吴静，李晓敏

《生物工程学报》

2026

代谢工程改造大肠杆菌利用葡萄糖和甲酸共底物合成L-苏氨酸

王梓暄，梁光杰，王家皓，高聪，刘佳，吴静

《生物工程学报》

DOI

2026

双酶级联高效催化对羟基亚苄基丙酮合成覆盆子酮

黄珊珊，靳阿原，宋伟，吴静

《微生物学报》

2026

L-组氨酸大肠杆菌细胞工厂构建与发酵优化

陈照阳，刘佳，董欣，胡贵鹏，周怡雯，吴静

《微生物学报》

2026

Synthetic Carbon-Efficient Pathway and Programmable Microenvironment for Sustainable Caffeic Acid Production

Binbing Xu, Qinyu Guo, Qi Cui, Wanqing Wei, Wei Song, Cong Gao, Xiaomin Li, Guangjie Liang, Kaifang Liu, Guipeng Hu, Jing Wu

ACS Sustainable Chemistry & Engineering

DOI

2026

Enhancing the Promiscuous Phosphorylation Activity of Acid Phosphatase via Transition-State-Stabilization-Mediated Screening Strategy

Wei Song, X X Xu, Wanqing Wei, Yiwen Zhou, Jing Wu

Journal of Agricultural and Food Chemistry

DOI

2026

Identification and Engineering of a β-1,3-Galactosyltransferase for Efficient Lacto- N -Tetraose Production

Wei Tao, Mengwei Zhang, Shengqi Gao, Luyao Wang, Zenglin Zhang, Jing Wu, Lingqia Su, Kang Zhang

Journal of Agricultural and Food Chemistry

DOI

2026

Rational Design of Tagatose 6-Phosphate 4-Epimerase for Efficient D -Tagatose Production Under Mg 2+ -Supplemented Conditions

Sheng Wang, Mengwei Lu, Demin Kong, Shiheng Chen, ZhiSheng Wei, Tianyu Liu, ZhiHao Li, Yinghao Huang, Zhengfei Yan, Z M Liu, Jing Wu, Wei Xia

Journal of Agricultural and Food Chemistry

DOI

2026

Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins

Xinyu Zhang, Zhanzhi Liu, Ying Xu, Mingjing Sun, Guangzhen Zhou, Deming Rao, Xiaoqian Chen, Sheng Wang, Zhigang Li, Yan Huang, Jing Wu

Advanced Science

DOI

2025

D-塔格糖3-差向异构酶的理性设计与机制解析

郭丁钰，魏婉清，宋伟，吴静，闻建，胡贵鹏，刘立明

《食品与发酵工业》

DOI

2025

大肠杆菌耐受性改造提高琥珀酸产量

潘静宇，唐永圣，刘佳，高聪，李晓敏，宋伟，魏婉清，胡贵鹏，吴静，刘立明，陈修来

《食品与生物技术学报》

2025

Mechanism‐Guided Protein Engineering of Paraburkholderia xenovorans Lactamase for the Green Synthesis of 2‐(2‐Oxopyrrolidin‐1‐yl)‐Butanoic Acid

Wenzhe Ma, Wanqing Wei, Jian Wen, Cong Gao, Guipeng Hu, Xiaomin Li, Jia Liu, Jing Wu, Wei Song

Biotechnology and Bioengineering

DOI

2025

Thermostable Coenzyme a Ligase for Efficient Biosynthesis of 2-Pyrrolidone via Protein and Fermentation Engineering

Bei Yu, Wei Song, Shenjie Wang, Wanqing Wei, Guipeng Hu, Xiaomin Li, Cong Gao, Jia Liu, Jian Wen, Jing Wu

ACS Synthetic Biology

DOI

2025

Multifunctions of Sustainable Chondroitin Sulfates with Predominant Subtypes and Low Molecular Weights on Neurite Outgrowth

Shuqin Xu, Meiling Qiu, Liyuan Liang, Yue Chen, Yajia Wang, Jing Wu, Jinghua Chen

Biomacromolecules

DOI

2025

Modeling Enzyme Temperature Stability from Sequence Segment Perspective

Ziqi Zhang, Shiheng Chen, Runze Yang, Zhisheng Wei, Wei Zhang, Lei Wang, Zhanzhi Liu, Fengshan Zhang, Jing Wu, Xiaoyong Pan, Hong‐Bin Shen, Longbing Cao, Zhaohong Deng

Journal of Chemical Information and Modeling

DOI

2025

Advancing Enzyme Optimal pH Prediction via Retrieved Embedding Data Augmentation

Ziqi Zhang, Zhisheng Wei, Zhengqiang Qin, Lei Wang, Jin‐Song Gong, Jin‐Song Shi, Jing Wu, Zhaohong Deng

Journal of Chemical Information and Modeling

DOI

2025

EMOCPD: Efficient Attention-Based Models for Computational Protein Design Using Amino Acid Microenvironment

Xiaoqi Ling, Cheng Cai, Demin Kong, Zhisheng Wei, Jing Wu, Lei Wang, Zhaohong Deng

Journal of Chemical Information and Modeling

DOI

2025

Thermostability Enhancement of Tagatose 4-Epimerase through Protein Engineering and Whole-Cell Immobilization

Zhanzhi Liu, Xuehong Guo, Ying Xu, Jing Wu

Journal of Agricultural and Food Chemistry

DOI

2024

基于量子力学分析β-内酰胺合成酶合成吡拉西坦中间体的机制

马文哲，宋伟，魏婉清，吴静，刘立明

《化工学报》

2024

酶法生产1,4-环己烷二甲胺

韩业挺，何志震，魏婉清，宋伟，刘立明，朱萌，吴静

《生物工程学报》

2024

代谢工程改造大肠杆菌底物利用途径促进L-赖氨酸生产

许雪晨，王浩淼，陈修来，吴静，高聪，宋伟，魏婉清，刘佳，柳亚迪，刘立明

《生物工程学报》

2024

“课程-项目-导师”交互提升生物工程类研究生创新能力的探索与实践

刘立明，李晓敏，吴静，高聪，宋伟

《生物工程学报》

2024

代谢工程改造大肠杆菌生产尸胺

刘存萍，高聪，李晓敏，陈修来，吴静，宋伟，魏婉清，刘立明

《生物工程学报》

2024

“六融六优”培养生物与医药专业研究生创新能力的探索与实践

吴静，周怡雯，宋伟，魏婉清，胡贵鹏，闻建，李晓敏，蒋艳，邱立朋

《生物工程学报》

2024

双酶级联催化L-色氨酸合成靛蓝

罗诗琪，魏婉清，吴静，宋伟，胡贵鹏，刘立明

《生物工程学报》

2024

生物催化氨基酸衍生化的关键技术

吴静

《中国轻工业出版社》

2024

Systems engineering Escherichia coli for efficient production p‐coumaric acid from glucose

Qiu Chong, Xiaoge Wang, Jiaojiao Zuo, Runyang Li, Cong Gao, Xiulai Chen, Jia Liu, Wanqing Wei, Jing Wu, Guipeng Hu, Wei Song, Nan Xu, Li Liu

Biotechnology and Bioengineering

DOI

2024

Genome‐Wide CRISPRi Screening of Key Genes for Recombinant Protein Expression in Bacillus Subtilis

Xuyang Zhu, Hui Luo, Xinrui Yu, Huihui Lv, Lingqia Su, Kang Zhang, Jing Wu

Advanced Science

DOI

2023

代谢工程改造大肠杆菌生产软骨素

赵春雷，郭亮，高聪，宋伟，吴静，刘佳，刘立明，陈修来

《化工学报》

2023

Protein engineering, cofactor engineering, and surface display engineering to achieve whole‐cell catalytic production of chondroitin sulfate A

Haoyu Liu, Wanqing Wei, Zixuan Pang, Simin Gu, Wei Song, Cong Gao, Xiulai Chen, Jia Liu, Liang Guo, Jing Wu, Li Liu

Biotechnology and Bioengineering

DOI

2023

Reprogramming Metabolic Flux in Escherichia Coli to Enhance Chondroitin Production

Chunlei Zhao, Xiaomin Li, Liang Guo, Cong Gao, Wei Song, Wanqing Wei, Jing Wu, Li Liu, Xiulai Chen

Advanced Science

DOI

2023

Engineered Artificial Membraneless Organelles in Saccharomyces cerevisiae To Enhance Chemical Production

Pei Zhou, Hui Liu, Xin Meng, Huiyun Zuo, Mengya Qi, Liang Guo, Cong Gao, Wei Song, Jing Wu, Xiulai Chen, Wei Chen, Li Liu

Angewandte Chemie

DOI

2022

生物催化氨基酸C—N裂解反应的研究进展

武耀运，陈城虎，宋伟，胡贵鹏，刘佳，吴静

《生物加工过程》

2022

多酶级联催化合成（R）-β-酪氨酸

宋伟，王金辉，胡贵鹏，陈修来，刘立明，吴静

《化工学报》

2022

Engineering Escherichia coli asymmetry distribution‐based synthetic consortium for shikimate production

Qiang Ding, Zhendong Li, Liang Guo, Wei Song, Jing Wu, Xiulai Chen, Li Liu, Cong Gao

Biotechnology and Bioengineering

DOI

2022

Efficient Production of L‐Homophenylalanine by Enzymatic‐Chemical Cascade Catalysis

Dengke Gao, Wei Song, Jing Wu, Liang Guo, Cong Gao, Jia Liu, Xiulai Chen, Li Liu

Angewandte Chemie

DOI

2022

A rice protein hydrolase from Serratia marcescens and its specificity in preparation of oligopeptide‐enriched rice protein hydrolysates

Zheng‐Fei Yan, Jian‐Qiao Zhou, Shuai Sammy Yuan, Cheng‐Ye Tang, Jing Wu

Food Bioengineering

DOI

2021

生物催化C—C成键反应及其应用

齐娜，宋伟，刘立明，吴静

《化工学报》

2021

生物催化C—N成键反应合成手性胺的研究进展

成雅琪，吴静，刘立明，宋伟

《化工学报》

2021

Engineering membrane asymmetry to increase medium‐chain fatty acid tolerance in Saccharomyces cerevisiae

Hui Liu, Weijia Yuan, Pei Zhou, Guangjie Liang, Cong Gao, Liang Guo, Guipeng Hu, Wei Song, Jing Wu, Xiulai Chen, Li Liu

Biotechnology and Bioengineering

DOI

2021

Enhanced Catalytic Efficiency of L‐amino Acid Deaminase Achieved by a Shorter Hydride Transfer Distance

Yaoyun Wu, Sheng Zhang, Wei Song, Jia Liu, Xiulai Chen, Guipeng Hu, Yiwen Zhou, Li Liu, Jing Wu

ChemCatChem

DOI

2021

Efficient Synthesis of D‐Phenylalanine from L‐Phenylalanine via a Tri‐Enzymatic Cascade Pathway

Cui Fen Lu, Zhisheng Zhang, Wei Song, Jia Liu, Xiulai Chen, Li Liu, Jing Wu

ChemCatChem

DOI

2020

Engineering protonation conformation of l ‐aspartate‐α‐decarboxylase to relieve mechanism‐based inactivation

Yuanyuan Qian, Cui Fen Lu, Jia Liu, Wei Song, Xiulai Chen, Qiuling Luo, Li Liu, Jing Wu

Biotechnology and Bioengineering

DOI

2020

Recombinant expression, characterization and application of maltotetraohydrolase from Pseudomonas saccharophila

Lingqia Su, Yanan Yang, Jing Wu

Journal of the Science of Food and Agriculture

DOI

2019

Efficient production of phenylpropionic acids by an amino‐group‐transformation biocatalytic cascade

Jinhui Wang, Wei Song, Jing Wu, Jia Liu, Xiulai Chen, Li Liu

Biotechnology and Bioengineering

DOI

2018

Highly efficient extracellular expression of naturally cytoplasmic Leuconostoc mesenteroides sucrose phosphorylase

Lingqia Su, Tian Wang, Lingang Yu, Jing Wu

Journal of Chemical Technology & Biotechnology

DOI

2017

Phosphoenolpyruvate:glucose phosphotransferase system modification increases the conversion rate during l -tryptophan production in Escherichia coli

Lina Liu, Sheng Chen, Jing Wu

Journal of Industrial Microbiology & Biotechnology

DOI

2017

Efficient production of (R)-3-TBDMSO glutaric acid methyl monoester by manipulating the substrate pocket of Pseudozyma antarctica lipase B

Jing Wu, Hongjiang Wang, Bin Yang, Wei Song, Chenchen Liang, Li Liu

RSC Advances

DOI

2015

Reconstruction and in silico analysis of an Actinoplanes sp. SE50/110 genome-scale metabolic model for acarbose production

Yali Wang, Nan Xu, Chao Ye, Li Liu, Zhongping Shi, Jing Wu

Frontiers in Microbiology

DOI

2015

Efficient extracellular expression of Bacillus deramificans pullulanase in Brevibacillus choshinensis

Chun Zou, Xuguo Duan, Jing Wu

Journal of Industrial Microbiology & Biotechnology

DOI

2015

Cutinase promotes dry esterification of cotton cellulose

Xiaoman Zhao, Teresa Matamá, Ribeiro Artur, Silva Carla, Jing Wu, Fu Jiajia, Artur Manuel Cavaco-Paulo

Biotechnology Progress

DOI

2014

Fumaric acid production by Torulopsis glabrata: Engineering the urea cycle and the purine nucleotide cycle

Xiulai Chen, Jing Wu, Wei Song, Limei Zhang, Hongjiang Wang, Li Liu

Biotechnology and Bioengineering

DOI

2014

A Unique Mono- and Diacylglycerol Lipase from Penicillium cyclopium: Heterologous Expression, Biochemical Characterization and Molecular Basis for Its Substrate Selectivity

Zhongbiao Tan, Jianfang Li, Xueting Li, Ying Gu, Minchen Wu, Jing Wu, Junqing Wang

PLoS ONE

DOI

2013

Enhanced expression of endoinulinase from Aspergillus niger by codon optimization in Pichia pastoris and its application in inulooligosaccharide production

Miao He, Dan Wu, Jing Wu, Jian Chen

Journal of Industrial Microbiology & Biotechnology

DOI

2013

Characterization of Sulfolobus solfataricus β ‐galactosidase mutant F441Y expressed in Pichia pastoris

Xiaojun Sun, Xuguo Duan, Dan Wu, Jian Chen, Jing Wu

Journal of the Science of Food and Agriculture

DOI

2013

Heterologous expression and biochemical characterization of an endo‐β‐1,4‐glucanase from Thermobifida fusca

Peng'an Yan, Lingqia Su, Jian Chen, Jing Wu

Biotechnology and Applied Biochemistry

DOI

2011

Development of l-tryptophan production strains by defined genetic modification in Escherichia coli

Zhijun Zhao, Chun Zou, Yixing Zhu, Jun Dai, Sheng Chen, Dan Wu, Jing Wu, Jian Chen

Journal of Industrial Microbiology & Biotechnology

DOI

2010

Combined use of mild oxidation and cutinase/lipase pretreatments for enzymatic processing of wool fabrics

Ping Wang, Li Cui, Qiang Wang, Xuerong Fan, Xianfei Zhao, Jing Wu

Engineering in Life Sciences

DOI

2009

Effects of cutinase on the enzymatic shrink-resist finishing of wool fabrics

Ping Wang, Qiang Wang, Xuerong Fan, Li Cui, Jiugang Yuan, Sheng Chen, Jing Wu

Enzyme and Microbial Technology

DOI

精选作品

期刊论文

构建整合型重组大肠杆菌高效合成软骨素

代谢工程改造大肠杆菌利用葡萄糖和甲酸共底物合成L-苏氨酸

双酶级联高效催化对羟基亚苄基丙酮合成覆盆子酮

L-组氨酸大肠杆菌细胞工厂构建与发酵优化

Synthetic Carbon-Efficient Pathway and Programmable Microenvironment for Sustainable Caffeic Acid Production

Enhancing the Promiscuous Phosphorylation Activity of Acid Phosphatase via Transition-State-Stabilization-Mediated Screening Strategy

Identification and Engineering of a β-1,3-Galactosyltransferase for Efficient Lacto- N -Tetraose Production

Rational Design of Tagatose 6-Phosphate 4-Epimerase for Efficient D -Tagatose Production Under Mg 2+ -Supplemented Conditions

Continuous Evolution System Based on Toxin–Antitoxin System Combined with Base Deaminase Accelerates the In Vivo Modification of Target Proteins

D-塔格糖3-差向异构酶的理性设计与机制解析

大肠杆菌耐受性改造提高琥珀酸产量

Mechanism‐Guided Protein Engineering of Paraburkholderia xenovorans Lactamase for the Green Synthesis of 2‐(2‐Oxopyrrolidin‐1‐yl)‐Butanoic Acid

Thermostable Coenzyme a Ligase for Efficient Biosynthesis of 2-Pyrrolidone via Protein and Fermentation Engineering

Multifunctions of Sustainable Chondroitin Sulfates with Predominant Subtypes and Low Molecular Weights on Neurite Outgrowth

Modeling Enzyme Temperature Stability from Sequence Segment Perspective

Advancing Enzyme Optimal pH Prediction via Retrieved Embedding Data Augmentation

EMOCPD: Efficient Attention-Based Models for Computational Protein Design Using Amino Acid Microenvironment

Thermostability Enhancement of Tagatose 4-Epimerase through Protein Engineering and Whole-Cell Immobilization

基于量子力学分析β-内酰胺合成酶合成吡拉西坦中间体的机制

酶法生产1,4-环己烷二甲胺

代谢工程改造大肠杆菌底物利用途径促进L-赖氨酸生产

“课程-项目-导师”交互提升生物工程类研究生创新能力的探索与实践

代谢工程改造大肠杆菌生产尸胺

“六融六优”培养生物与医药专业研究生创新能力的探索与实践

双酶级联催化L-色氨酸合成靛蓝

生物催化氨基酸衍生化的关键技术

Systems engineering Escherichia coli for efficient production p‐coumaric acid from glucose

Genome‐Wide CRISPRi Screening of Key Genes for Recombinant Protein Expression in Bacillus Subtilis

代谢工程改造大肠杆菌生产软骨素

Protein engineering, cofactor engineering, and surface display engineering to achieve whole‐cell catalytic production of chondroitin sulfate A

Reprogramming Metabolic Flux in Escherichia Coli to Enhance Chondroitin Production

Engineered Artificial Membraneless Organelles in Saccharomyces cerevisiae To Enhance Chemical Production

生物催化氨基酸C—N裂解反应的研究进展

多酶级联催化合成（R）-β-酪氨酸

Engineering Escherichia coli asymmetry distribution‐based synthetic consortium for shikimate production

Efficient Production of L‐Homophenylalanine by Enzymatic‐Chemical Cascade Catalysis

A rice protein hydrolase from Serratia marcescens and its specificity in preparation of oligopeptide‐enriched rice protein hydrolysates

生物催化C—C成键反应及其应用

生物催化C—N成键反应合成手性胺的研究进展

Engineering membrane asymmetry to increase medium‐chain fatty acid tolerance in Saccharomyces cerevisiae

Enhanced Catalytic Efficiency of L‐amino Acid Deaminase Achieved by a Shorter Hydride Transfer Distance

Efficient Synthesis of D‐Phenylalanine from L‐Phenylalanine via a Tri‐Enzymatic Cascade Pathway

Engineering protonation conformation of l ‐aspartate‐α‐decarboxylase to relieve mechanism‐based inactivation

Recombinant expression, characterization and application of maltotetraohydrolase from Pseudomonas saccharophila

Efficient production of phenylpropionic acids by an amino‐group‐transformation biocatalytic cascade

Highly efficient extracellular expression of naturally cytoplasmic Leuconostoc mesenteroides sucrose phosphorylase

Phosphoenolpyruvate:glucose phosphotransferase system modification increases the conversion rate during l -tryptophan production in Escherichia coli

Efficient production of (R)-3-TBDMSO glutaric acid methyl monoester by manipulating the substrate pocket of Pseudozyma antarctica lipase B

Reconstruction and in silico analysis of an Actinoplanes sp. SE50/110 genome-scale metabolic model for acarbose production

Efficient extracellular expression of Bacillus deramificans pullulanase in Brevibacillus choshinensis

Cutinase promotes dry esterification of cotton cellulose

Fumaric acid production by Torulopsis glabrata: Engineering the urea cycle and the purine nucleotide cycle

A Unique Mono- and Diacylglycerol Lipase from Penicillium cyclopium: Heterologous Expression, Biochemical Characterization and Molecular Basis for Its Substrate Selectivity

Enhanced expression of endoinulinase from Aspergillus niger by codon optimization in Pichia pastoris and its application in inulooligosaccharide production

Characterization of Sulfolobus solfataricus β ‐galactosidase mutant F441Y expressed in Pichia pastoris

Heterologous expression and biochemical characterization of an endo‐β‐1,4‐glucanase from Thermobifida fusca

Development of l-tryptophan production strains by defined genetic modification in Escherichia coli

Combined use of mild oxidation and cutinase/lipase pretreatments for enzymatic processing of wool fabrics

Effects of cutinase on the enzymatic shrink-resist finishing of wool fabrics

研究方向