李爱涛｜湖北大学｜学者主页

2026

祖先序列重建与理性设计赋能非特异性过加氧酶实现高效甾体母核氧化官能团化（英文）

胡瑞文，郭志勇，彭小刚，石晓琪，乔玉本，李倩，高成华，李爱涛

Chinese Journal of Catalysis

2026

Engineering the Enzyme−Support Interface: Arginine-Directed Anchoring of Peroxygenase on a β-Ketoester Covalent Organic Framework

吳宛錚，Yikang Lu，Aitao Li，Yuxia Pang，Hongming Lou，Xueqing Qiu，Zhixian Li

ACS Applied Materials & Interfaces

DOI

2026

A Pioneer of Directed Evolution in Catalysis: Manfred T. Reetz (1943‐2026)

Carlos G. Acevedo‐Rocha, Yosephine Gumulya, Frank Hollmann, Aitao Li, Régis Mondière, Diederik Johannes Opperman, Joaquı́n Sanchis, Zhoutong Sun, Andreas Taglieber, Andreas Vogel

Angewandte Chemie

DOI

2026

Unspecific Peroxygenase Catalyzed Benzylic C–H Functionalization of di N -Heterocycles and Its Application

D H Deng, Meimei Kong, Jingyu Wang, Lixin Kang, Yuben Qiao, Aitao Li

Organic Letters

DOI

2026

Mesoporous silica nanoparticles encapsulating xylanase: Efficient platform for low-polymerization-degree xylooligosaccharides preparation

Xuefei Zhu, Aitao Li, Caoxing Huang, Yingjie Du, Qiang Yong

Bioresource Technology

DOI

2026

Multidimensional Engineering Delivers a High-Performance C11α-Steroid Hydroxylase for Practical Application

Lingrui Wei, 郭志勇, Yingying Li, Jiahui Wu, Ling Wang, Pan Xiao, Chenghua Gao, Qian Li, Aitao Li

Journal of Agricultural and Food Chemistry

DOI

2026

Lid loop-mediated proton transfer revealed in the Fe/αKG-dependent decarboxylase TraH

Xuehua Zheng, Rui Ge, Zhiyong Guo, Mathias Girbig, Johannes Freitag, Aitao Li, Georg K. A. Hochberg, Shu‐Ming Li, Gert Bange, Liujuan Zheng

Communications Chemistry

DOI

2026

A distinct zearalenone detoxification strategy mediated by cytochrome P450

Ziwei Liu, Rouming Peng, Qian Li, Chun-Chi Chen, Lilan Xin, Kangkang Li, Jian‐Wen Huang, Xin Bo Liu, Xiaodong Zhang, Linzhen Hu, Shangwen Luo, Haibing Zhou, Aitao Li, Jian Min, Rey-Ting Guo

Food Chemistry

DOI

2026

A plug-and-play cytochrome P450 system enables de novo biosynthesis of 2-phenylethanol derivatives

Yuanqing Wu, Yue Wang, Yan Lv, Xiaodong Zhang, Aitao Li

Metabolic Engineering

DOI

2026

Synergistic integration of ancestral sequence reconstruction and rational design empowers unspecific peroxygenase for efficient steroid core oxyfunctionalization

Ruiwen Hu, Zhiyong Guo, Xiaogang Peng, Xiaoqi Shi, Yuben Qiao, Qian Li, Chenghua Gao, Aitao Li

CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION)

DOI

2025

Molecular insights into the substrate recognition and quasi-unidirectional catalysis of OpaE, an atypical fungal enzyme from the Asp/Glu racemase family

Xuehua Zheng, Zhi-Yong Guo, Le-Yao Chen, Haowen Wang, Johannes Freitag, Aitao Li, G. Bange, Shu-Ming Li, Liu-Juan Zheng

Journal of Biological Chemistry

DOI

2025

Structural and catalytic insights into MhpB: A dioxygenase enzyme for degrading catecholic pollutants.

Xu Dong, Manli Xu, Miao Wu, Ying Wang, Xiaoqi Cheng, Wenxue Jiang, Dule Zheng, Ahmed Omar, Yibin Cheng, Aitao Li, Lixin Ma, Qiong Xing

Journal of Hazardous Materials

DOI

2025

Proximal-to-distal molecular engineering of a halide methyltransferase for green synthesis of S-adenosyl-ethionine

Shi-Yu Zhang, Jun-Yi Yang, Yan Yang, Lixin Kang, Aitao Li, Jian‐He Xu, Gao‐Wei Zheng, Qi Chen

Molecular Catalysis

DOI

2025

Divergent evolution of fungal P450 monooxygenase unlocks simultaneous access to C12β and C15α oxyfunctionalization of steroids

Ling Wang, Ying Wang, Xiaogang Peng, Chenghua Gao, Xinfang Wei, Peng Chen, Pan Xiao, Qian Li, Aitao Li

Nature Communications

DOI

2025

Compartmentalized orthogonal biosynthesis for boosting limonene and oxygenated monoterpenoid production in Saccharomyces cerevisiae

Aoqing Ma, Huili Yu, Aitao Li

Bioresource Technology

DOI

2025

Structure-Guided Engineering of CYP109B4: From Monooxygenase to Peroxygenase for Selective Steroid Hydroxylation

Xiaodong Zhang, Yongchao Wang, Xiaoqing Jiang, Di Deng, Qian Li, Binju Wang, Aitao Li

Journal of Agricultural and Food Chemistry

DOI

2025

Biocatalytic Conversion of Caprolactam to 1,6-Hexanediamine: A Novel Route for Nylon Monomer Production

Zhongwei Zhang, J.W. Chen, Ying Wang, Aitao Li

ACS Catalysis

DOI

2025

Rational Mining and Engineering of Unique P450 Enzymes Enable Production of Diverse Dimeric Tryptophan-Containing Diketopiperazine Alkaloids

Huili Yu, Lei Hu, Xin Ruan, Xiaogang Peng, Pan Xiao, Ningjia Ao, Peng Chen, Chao Yang, Shu‐Ming Li, Zhiyong Guo, Aitao Li

ACS Catalysis

DOI

2025

Integrated protein and metabolic engineering enable high-yield biosynthesis of resveratrol derivatives in Yarrowia lipolytica

Yuanqing Wu, Shuifeng Zheng, Zhibin Yang, Xiaodong Zhang, Rusong Li, Aitao Li

Synthetic and Systems Biotechnology

DOI

2025

Applications of cytochrome P450 monooxygenases in the biosynthesis of active pharmaceutical ingredients

Yuben Qiao, Xiaodong Zhang, Qianqian Xu, Ikuro Abe, Aitao Li

Green Synthesis and Catalysis

DOI

2025

Efficient synthesis of clascoterone via lipase-catalyzed solvent-free alcoholysis

Qian Li, Mingpeng Li, Ling Wang, Xiaojuan Yu, Yuling Zhou, Chenghua Gao, Aitao Li

BioDesign Research

DOI

2025

Regioselectivity Switching in CYP107Pdh-Catalyzed VD3 Hydroxylation: A Structure-Guided Approach To Improve Calcidiol Production

Lixin Kang, Haoran Li, Kexin Lin, Shen Hu, Song Liu, Yuben Qiao, Ying Wang, Aitao Li

ACS Catalysis

DOI

2025

Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane

Fei Wang, Huiqi Sun, Di Deng, Yuanqing Wu, Jing Bo Zhao, Qian Li, Aitao Li

Advanced Science

DOI

2025

An efficient catalytic route in haem peroxygenases mediated by O2/small-molecule reductant pairs for sustainable applications

Di Deng, Zhihui Jiang, Lixin Kang, Langxing Liao, Xiaodong Zhang, Yuben Qiao, Yang Zhou, Liulin Yang, Binju Wang, Aitao Li

Nature Catalysis

DOI

2024

d-Allulose production via a simplified in vitro multienzyme cascade strategy: Biosynthesis and crystallization

Han Zhang, Zhen Sun, Xinling Du, Qianqian Cheng, Fuchun Ji, Zishen Nie, Julei Zhan, Zhouping Wang, Aitao Li, I. Delidovich, Yu Xia

Food Bioscience

DOI

2024

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Yuanqing Wu, Shuocheng Li, Baijian Sun, Jingyi Guo, Meiyi Zheng, Aitao Li

ACS Synthetic Biology

DOI

2024

Mechanistic Insights into the Selective C–S Bond Formation by P450 TleB

Hongxun Gao, Yakun Fan, Xuwei He, Xiaogang Peng, Zhen Li, Yanxin Zheng, Shengbiao Ji, Long‐Wu Ye, Aitao Li, Binju Wang, Jing Bo Zhao

ACS Catalysis

DOI

2024

Modulating regioselectivity of CYP107J3-catalyzed isophorone hydroxylation by disrupting the hydrophobic balance of the substrate binding pocket

Meng Dai, Xiaojuan Yu, Xiaogang Peng, Zhiyong Guo, Huili Yu, Aitao Li

Green Synthesis and Catalysis

DOI

2024

Food-Grade Expression of Two Laccases in Pichia pastoris and Study on Their Enzymatic Degradation Characteristics for Mycotoxins

Zhen Sun, Yingxin You, Huidong Xu, Yang You, Wenjing He, Zhouping Wang, Aitao Li, Yu Xia

Journal of Agricultural and Food Chemistry

DOI

2024

Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910

Xin Fu, Kexin Lin, Xiaodong Zhang, Zhiyong Guo, Lixin Kang, Aitao Li

Bioresources and Bioprocessing

DOI

2024

Front Cover: Development of a Thermodynamically Favorable Multi‐enzyme Cascade Reaction for Efficient Sustainable Production of ω‐Amino Fatty Acids and α,ω‐Diamines (ChemSusChem 6/2024)

Ju‐Mou Li, Kun Shi, Aitao Li, Zhi‐Jun Zhang, Hui‐Lei Yu, Jian‐He Xu

ChemSusChem

DOI

2023

细胞色素P450酶在香精香料绿色生物合成中的应用

郁慧丽，李爱涛

《生物技术通报》

2023

苯乙烯及其衍生物的生物氨羟化反应用于β-氨基醇的高效合成（英文）

胡瑞文，龚安界，廖浪星，郑颜欣，刘鑫，吴鹏，李福帅，郁慧丽，赵晶，叶龙武，王斌举，李爱涛

Chinese Journal of Catalysis

2023

细胞色素P450 Kemp消除酶的改造及新型催化机制解析（英文）

李爱涛，汪倩，宋希彤，张小栋，黄建文，陈纯琪，郭瑞庭，王斌举，Manfred T.Reetz

Chinese Journal of Catalysis

2023

Development of a Thermodynamically Favorable Multi‐enzyme Cascade Reaction for Efficient Sustainable Production of ω‐Amino Fatty Acids and α,ω‐Diamines

Ju‐Mou Li, Kun Shi, Aitao Li, Zhi‐Jun Zhang, Hui‐Lei Yu, Jian‐He Xu

ChemSusChem

DOI

2023

A Novel Strategy for Whole-Cell Biotransformation Enabling Simultaneous l -Phenyllactic Acid Production and Coenzyme Regeneration

Huidong Xu, Qianqian Cheng, Yangyu Qiu, Jingjing Mao, Qinyi Ji, Mulan Zhu, Lili Zhang, Zhouping Wang, Aitao Li, Yu Xia

Journal of Agricultural and Food Chemistry

DOI

2023

A Designed Chemoenzymatic Route for Efficient Synthesis of 6-Dehydronandrolone Acetate: A Key Precursor in the Synthesis of C7-Functionalized Steroidal Drugs

Zili Zhang, Chenghua Gao, Jing Bo Zhao, Xiaogang Peng, Qian Li, Aitao Li

ACS Catalysis

DOI

2023

Hydroxylases involved in terpenoid biosynthesis: a review

Zihan Zhang, Qing‐Yang Wu, Yue Ge, Zhengyu Huang, Ran Hong, Aitao Li, Jian‐He Xu, Hui‐Lei Yu

Bioresources and Bioprocessing

DOI

2023

Directed Synthesis of Biobased 1,6-Diaminohexane from Adipic Acid by Rational Regulation of a Functional Enzyme Cascade in Escherichia coli

Li Wang, Guohui Li, Aitao Li, Yu Deng

ACS Sustainable Chemistry & Engineering

DOI

2023

Engineering of a P450-based Kemp eliminase with a new mechanism

Aitao Li, Qian Wang, Qian Wang, Xitong Song, Xiaodong Zhang, Jian-Wen Huang, Chun‐Chi Chen, Rey‐Ting Guo, Binju Wang, Manfred T. Reetz

CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION)

DOI

2023

A Fungal P450 Enzyme from Fusarium graminearum with Unique 12β-Steroid Hydroxylation Activity

Ling Wang, Xiaowei Wu, Chenghua Gao, Lingrui Wei, Qian Li, Aitao Li

Applied and Environmental Microbiology

DOI

2023

Transforming Inert Cycloalkanes into α,ω‐Diamines by Designed Enzymatic Cascade Catalysis

Zhongwei Zhang, Lin Fang, Fei Wang, Yu Deng, Zhengbin Jiang, Aitao Li

Angewandte Chemie

DOI

2023

Rationally Controlling Selective Steroid Hydroxylation via Scaffold Sampling of a P450 Family

Xiaodong Zhang, Pan‐Pan Shen, Jing Bo Zhao, Yueyue Chen, Xian Li, Jian‐Wen Huang, Lilan Zhang, Qian Li, Chenghua Gao, Qiong Xing, Chun‐Chi Chen, Rey‐Ting Guo, Aitao Li

ACS Catalysis

DOI

2022

Biocatalytic aminohydroxylation of styrenes for efficient synthesis of enantiopure β-amino alcohols

Ruiwen Hu, Anjie Gong, Langxing Liao, Yanxin Zheng, Xin Liu, Peng Wu, Fushuai Li, Huili Yu, Jing Bo Zhao, Long‐Wu Ye, Binju Wang, Aitao Li

CHINESE JOURNAL OF CATALYSIS (CHINESE VERSION)

DOI

2022

Building Flexible Escherichia coli Modules for Bifunctionalizing n-Octanol: The Byproduct of Oleic Acid Biorefinery

Gang-Gang Chong, Liang‐Yi Ding, Yan‐Yan Qiu, Xiao‐Long Qian, Ya‐Li Dong, Chun‐Xiu Li, Aitao Li, Jiang Pan, Jian‐He Xu

Journal of Agricultural and Food Chemistry

DOI

2022

Carving the Active Site of CYP153A7 Monooxygenase for Improving Terminal Hydroxylation of Medium‐Chain Fatty Acids

Ya‐Li Dong, Gang-Gang Chong, Chun‐Xiu Li, Qi Chen, Jiang Pan, Aitao Li, Jian‐He Xu

ChemBioChem

DOI

2022

A Chemoenzymatic Strategy for the Synthesis of Steroid Drugs Enabled by P450 Monooxygenase-Mediated Steroidal Core Modification

Yaqin Peng, Chenghua Gao, Zili Zhang, Shijie Wu, Jing Bo Zhao, Aitao Li

ACS Catalysis

DOI

2022

Molecular Basis for a Toluene Monooxygenase to Govern Substrate Selectivity

Chun‐Chi Chen, Meng Dai, Lilan Zhang, Jing Bo Zhao, Wei Zeng, Min Jun Shi, Jian‐Wen Huang, Weidong Liu, Rey‐Ting Guo, Aitao Li

ACS Catalysis

DOI

2022

Recent advances in the sustainable production of α,ω-C6 bifunctional compounds enabled by chemo-/biocatalysts

Qian Li, Zhongwei Zhang, Jing Bo Zhao, Aitao Li

Green Chemistry

DOI

2021

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

Zhiwei Zhang, Yang Liu, Jing Bo Zhao, Wenqiang Li, Ruiwen Hu, Xia Li, Aitao Li, Yaping Wang, Lixin Ma

BMC Biotechnology

DOI

2021

Mining methods and typical structural mechanisms of terpene cyclases

Zhengyu Huang, Ru-Yi Ye, Hui‐Lei Yu, Aitao Li, Jian‐He Xu

Bioresources and Bioprocessing

DOI

2021

Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics

Carlos G. Acevedo‐Rocha, Aitao Li, Lorenzo D’Amore, Sabrina Hoebenreich, Joaquı́n Sanchis, Paul Lubrano, Matteo Paolo Ferla, Marc Garcia‐Borràs, Sílvia Osuna, Manfred T. Reetz

Nature Communications

DOI

2021

Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio—and Stereoselective Steroid Hydroxylation

Xiaodong Zhang, Yun Fu Hu, Wei Jun Peng, Chenghua Gao, Qiong Xing, Binju Wang, Aitao Li

Frontiers in Chemistry

DOI

2020

Reductive Amination of Biobased Levulinic Acid to Unnatural Chiral γ-Amino Acid Using an Engineered Amine Dehydrogenase

Rui-Feng Cai, Lei Liu, Fei‐Fei Chen, Aitao Li, Jian‐He Xu, Gao‐Wei Zheng

ACS Sustainable Chemistry & Engineering

DOI

2020

One-pot biocatalytic route from cycloalkanes to α,ω‐dicarboxylic acids by designed Escherichia coli consortia

Fei Wang, Jing Bo Zhao, Qian Li, Jun Yang, Renjie Li, Jian Min, Xiaojuan Yu, Gao‐Wei Zheng, Hui‐Lei Yu, Chao Zhai, Carlos G. Acevedo‐Rocha, Lixin Ma, Aitao Li

Nature Communications

DOI

2020

Biosynthesis of organic molecules via artificial cascade reactions based on cytochrome P450 monooxygenases

Ren‐Jie Li, Zhongwei Zhang, Carlos G. Acevedo‐Rocha, Jing Bo Zhao, Aitao Li

Green Synthesis and Catalysis

DOI

2020

Regio‐ and Stereoselective Steroid Hydroxylation at C7 by Cytochrome P450 Monooxygenase Mutants

Aitao Li, Carlos G. Acevedo‐Rocha, Lorenzo D’Amore, Jinfeng Chen, Yaqin Peng, Marc Garcia‐Borràs, Chenghua Gao, Jinmei Zhu, Harry Rickerby, Sílvia Osuna, Jiahai Zhou, Manfred T. Reetz

Angewandte Chemie

DOI

2020

A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method

Mengjie Dong, Fei Wang, Qingqing Li, Rui Han, Aitao Li, Chao Zhai, Lixin Ma

Applied Microbiology and Biotechnology

DOI

2020

Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design

Xiaodong Zhang, Yaqin Peng, Jing Bo Zhao, Qian Li, Xiaojuan Yu, Carlos G. Acevedo‐Rocha, Aitao Li

Bioresources and Bioprocessing

DOI

2020

One-pot biosynthesis of 1,6-hexanediol from cyclohexane by de novo designed cascade biocatalysis

Zhongwei Zhang, Qian Li, Fei Wang, Renjie Li, Xiaojuan Yu, Lixin Kang, Jing Bo Zhao, Aitao Li

Green Chemistry

DOI

2019

Attenuated substrate inhibition of a haloketone reductase via structure-guided loop engineering

Yue‐Peng Shang, Qi Chen, Aitao Li, Shu Quan, Jian‐He Xu, Hui‐Lei Yu

Journal of Biotechnology

DOI

2019

Chemoenzymatic Conversion of Corncob to Furfurylamine via Tandem Catalysis with Tin-Based Solid Acid and Transaminase Biocatalyst

Pengqi Zhang, Xiaolong Liao, Cuiluan Ma, Qing Li, Aitao Li, Yu-Cai He

ACS Sustainable Chemistry & Engineering

DOI

2019

Chemical and Biocatalytic Routes to Arbutin †

Hangyu Zhou, Jing Bo Zhao, Aitao Li, Manfred T. Reetz

Molecules

DOI

2019

Engineering an Alcohol Dehydrogenase for Balancing Kinetics in NADPH Regeneration with 1,4-Butanediol as a Cosubstrate

Guochao Xu, Cheng Zhu, Aitao Li, Yan Ni, Yan Ni, Ruizhi Han, Jieyu Zhou, Ye Ni, Ye Ni

ACS Sustainable Chemistry & Engineering

DOI

2019

Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets

Aitao Li, Ge Qu, Zhoutong Sun, Manfred T. Reetz

ACS Catalysis

DOI

2019

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

Ge Qu, Aitao Li, Carlos G. Acevedo‐Rocha, Zhoutong Sun, Manfred T. Reetz

Angewandte Chemie International Edition

DOI

2019

Die zentrale Rolle der Methodenentwicklung in der gerichteten Evolution selektiver Enzyme

Ge Qu, Aitao Li, Carlos G. Acevedo‐Rocha, Zhoutong Sun, Manfred T. Reetz

Angewandte Chemie

DOI

2019

An effective hybrid strategy for converting rice straw to furoic acid by tandem catalysis via Sn-sepiolite combined with recombinant E. coli whole cells harboring horse liver alcohol dehydrogenase

Bo Peng, Cuiluan Ma, Peng-Qi Zhang, Changqing Wu, Ziwei Wang, Aitao Li, Yu-Cai He, Bin Yang

Green Chemistry

DOI

2018

Rapid and Error-Free Site-Directed Mutagenesis by a PCR-Free In Vitro CRISPR/Cas9-Mediated Mutagenic System

Wenwen She, Jing Ni, Ke Shui, Fei Wang, Ruyi He, Jinhui Xue, Manfred T. Reetz, Aitao Li, Lixin Ma

ACS Synthetic Biology

DOI

2018

Back Cover: Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase (Angew. Chem. Int. Ed. 3/2019)

Hangyu Zhou, Binju Wang, Fei Wang, Xiaojuan Yu, Lixin Ma, Aitao Li, Manfred T. Reetz

Angewandte Chemie International Edition

DOI

2018

Rücktitelbild: Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase (Angew. Chem. 3/2019)

Hangyu Zhou, Binju Wang, Fei Wang, Xiaojuan Yu, Lixin Ma, Aitao Li, Manfred T. Reetz

Angewandte Chemie

DOI

2018

Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase

Hangyu Zhou, Binju Wang, Fei Wang, Xiaojuan Yu, Lixin Ma, Aitao Li, Manfred T. Reetz

Angewandte Chemie

DOI

2018

Solid‐Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution?

Aitao Li, Zhoutong Sun, Manfred T. Reetz

ChemBioChem

DOI

2018

Enhancing the Catalytic Performance of a CYP116B Monooxygenase by Transdomain Combination Mutagenesis

Ren‐Jie Li, Jian‐He Xu, Qi Chen, Jing Bo Zhao, Aitao Li, Hui‐Lei Yu

ChemCatChem

DOI

2018

Front Cover: Enhancing the Catalytic Performance of a CYP116B Monooxygenase by Transdomain Combination Mutagenesis (ChemCatChem 14/2018)

Ren‐Jie Li, Jian‐He Xu, Qi Chen, Jing Bo Zhao, Aitao Li, Hui‐Lei Yu

ChemCatChem

DOI

2018

Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy

Aitao Li, Carlos G. Acevedo‐Rocha, Manfred T. Reetz

Applied Microbiology and Biotechnology

DOI

2018

P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping

Carlos G. Acevedo‐Rocha, Charles G. Gamble, Richard Lonsdale, Aitao Li, Nathalie Nett, Sabrina Hoebenreich, Julia B. Lingnau, C. Wirtz, Christophe Farès, Heike Hinrichs, Alfred Deege, Adrian John Mulholland, Yuval Nov, David Leys, Kirsty J. McLean, Andrew W. Munro, Manfred T. Reetz

ACS Catalysis

DOI

2018

Bioamination of alkane with ammonium by an artificially designed multienzyme cascade

Hui‐Lei Yu, Tuo Li, Fei‐Fei Chen, Xiaojing Luo, Aitao Li, Chao Yang, Gao‐Wei Zheng, Jian‐He Xu

Metabolic Engineering

DOI

2018

Front Cover: Beating Bias in the Directed Evolution of Proteins: Combining High‐Fidelity on‐Chip Solid‐Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction (ChemBioChem 3/2018)

Aitao Li, Carlos G. Acevedo‐Rocha, Zhoutong Sun, Tony Cox, Jia Lucy Xu, Manfred T. Reetz

ChemBioChem

DOI

2018

Engineering P450 LaMO stereospecificity and product selectivity for selective C–H oxidation of tetralin-like alkylbenzenes

Ren‐Jie Li, Aitao Li, Jing Bo Zhao, Qi Chen, Ning Li, Hui‐Lei Yu, Jian‐He Xu

Catalysis Science & Technology

DOI

2017

Beating Bias in the Directed Evolution of Proteins: Combining High‐Fidelity on‐Chip Solid‐Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction

Aitao Li, Carlos G. Acevedo‐Rocha, Zhoutong Sun, Tony Cox, Jia Lucy Xu, Manfred T. Reetz

ChemBioChem

DOI

2017

One-pot conversion of biomass-derived xylose to furfuralcohol by a chemo-enzymatic sequential acid-catalyzed dehydration and bioreduction

Yu-Cai He, Yun Ding, Cuiluan Ma, Junhua Di, Chunxia Jiang, Aitao Li

Green Chemistry

DOI

精选作品

期刊论文

祖先序列重建与理性设计赋能非特异性过加氧酶实现高效甾体母核氧化官能团化（英文）

Engineering the Enzyme−Support Interface: Arginine-Directed Anchoring of Peroxygenase on a β-Ketoester Covalent Organic Framework

A Pioneer of Directed Evolution in Catalysis: Manfred T. Reetz (1943‐2026)

Unspecific Peroxygenase Catalyzed Benzylic C–H Functionalization of di N -Heterocycles and Its Application

Mesoporous silica nanoparticles encapsulating xylanase: Efficient platform for low-polymerization-degree xylooligosaccharides preparation

Multidimensional Engineering Delivers a High-Performance C11α-Steroid Hydroxylase for Practical Application

Lid loop-mediated proton transfer revealed in the Fe/αKG-dependent decarboxylase TraH

A distinct zearalenone detoxification strategy mediated by cytochrome P450

A plug-and-play cytochrome P450 system enables de novo biosynthesis of 2-phenylethanol derivatives

Synergistic integration of ancestral sequence reconstruction and rational design empowers unspecific peroxygenase for efficient steroid core oxyfunctionalization

Molecular insights into the substrate recognition and quasi-unidirectional catalysis of OpaE, an atypical fungal enzyme from the Asp/Glu racemase family

Structural and catalytic insights into MhpB: A dioxygenase enzyme for degrading catecholic pollutants.

Proximal-to-distal molecular engineering of a halide methyltransferase for green synthesis of S-adenosyl-ethionine

Divergent evolution of fungal P450 monooxygenase unlocks simultaneous access to C12β and C15α oxyfunctionalization of steroids

Compartmentalized orthogonal biosynthesis for boosting limonene and oxygenated monoterpenoid production in Saccharomyces cerevisiae

Structure-Guided Engineering of CYP109B4: From Monooxygenase to Peroxygenase for Selective Steroid Hydroxylation

Biocatalytic Conversion of Caprolactam to 1,6-Hexanediamine: A Novel Route for Nylon Monomer Production

Rational Mining and Engineering of Unique P450 Enzymes Enable Production of Diverse Dimeric Tryptophan-Containing Diketopiperazine Alkaloids

Integrated protein and metabolic engineering enable high-yield biosynthesis of resveratrol derivatives in Yarrowia lipolytica

Applications of cytochrome P450 monooxygenases in the biosynthesis of active pharmaceutical ingredients

Efficient synthesis of clascoterone via lipase-catalyzed solvent-free alcoholysis

Regioselectivity Switching in CYP107Pdh-Catalyzed VD3 Hydroxylation: A Structure-Guided Approach To Improve Calcidiol Production

Multidimensional Engineering of Escherichia coli for Efficient Adipic Acid Synthesis From Cyclohexane

An efficient catalytic route in haem peroxygenases mediated by O2/small-molecule reductant pairs for sustainable applications

d-Allulose production via a simplified in vitro multienzyme cascade strategy: Biosynthesis and crystallization

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Mechanistic Insights into the Selective C–S Bond Formation by P450 TleB

Modulating regioselectivity of CYP107J3-catalyzed isophorone hydroxylation by disrupting the hydrophobic balance of the substrate binding pocket

Food-Grade Expression of Two Laccases in Pichia pastoris and Study on Their Enzymatic Degradation Characteristics for Mycotoxins

Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910

Front Cover: Development of a Thermodynamically Favorable Multi‐enzyme Cascade Reaction for Efficient Sustainable Production of ω‐Amino Fatty Acids and α,ω‐Diamines (ChemSusChem 6/2024)

细胞色素P450酶在香精香料绿色生物合成中的应用

苯乙烯及其衍生物的生物氨羟化反应用于β-氨基醇的高效合成（英文）

细胞色素P450 Kemp消除酶的改造及新型催化机制解析（英文）

Development of a Thermodynamically Favorable Multi‐enzyme Cascade Reaction for Efficient Sustainable Production of ω‐Amino Fatty Acids and α,ω‐Diamines

A Novel Strategy for Whole-Cell Biotransformation Enabling Simultaneous l -Phenyllactic Acid Production and Coenzyme Regeneration

A Designed Chemoenzymatic Route for Efficient Synthesis of 6-Dehydronandrolone Acetate: A Key Precursor in the Synthesis of C7-Functionalized Steroidal Drugs

Hydroxylases involved in terpenoid biosynthesis: a review

Directed Synthesis of Biobased 1,6-Diaminohexane from Adipic Acid by Rational Regulation of a Functional Enzyme Cascade in Escherichia coli

Engineering of a P450-based Kemp eliminase with a new mechanism

A Fungal P450 Enzyme from Fusarium graminearum with Unique 12β-Steroid Hydroxylation Activity

Transforming Inert Cycloalkanes into α,ω‐Diamines by Designed Enzymatic Cascade Catalysis

Rationally Controlling Selective Steroid Hydroxylation via Scaffold Sampling of a P450 Family

Biocatalytic aminohydroxylation of styrenes for efficient synthesis of enantiopure β-amino alcohols

Building Flexible Escherichia coli Modules for Bifunctionalizing n-Octanol: The Byproduct of Oleic Acid Biorefinery

Carving the Active Site of CYP153A7 Monooxygenase for Improving Terminal Hydroxylation of Medium‐Chain Fatty Acids

A Chemoenzymatic Strategy for the Synthesis of Steroid Drugs Enabled by P450 Monooxygenase-Mediated Steroidal Core Modification

Molecular Basis for a Toluene Monooxygenase to Govern Substrate Selectivity

Recent advances in the sustainable production of α,ω-C6 bifunctional compounds enabled by chemo-/biocatalysts

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

Mining methods and typical structural mechanisms of terpene cyclases

Pervasive cooperative mutational effects on multiple catalytic enzyme traits emerge via long-range conformational dynamics

Exploring the Potential of Cytochrome P450 CYP109B1 Catalyzed Regio—and Stereoselective Steroid Hydroxylation

Reductive Amination of Biobased Levulinic Acid to Unnatural Chiral γ-Amino Acid Using an Engineered Amine Dehydrogenase

One-pot biocatalytic route from cycloalkanes to α,ω‐dicarboxylic acids by designed Escherichia coli consortia

Biosynthesis of organic molecules via artificial cascade reactions based on cytochrome P450 monooxygenases

Regio‐ and Stereoselective Steroid Hydroxylation at C7 by Cytochrome P450 Monooxygenase Mutants

A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method

Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design

One-pot biosynthesis of 1,6-hexanediol from cyclohexane by de novo designed cascade biocatalysis

Attenuated substrate inhibition of a haloketone reductase via structure-guided loop engineering

Chemoenzymatic Conversion of Corncob to Furfurylamine via Tandem Catalysis with Tin-Based Solid Acid and Transaminase Biocatalyst

Chemical and Biocatalytic Routes to Arbutin †

Engineering an Alcohol Dehydrogenase for Balancing Kinetics in NADPH Regeneration with 1,4-Butanediol as a Cosubstrate

Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets

The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes

Die zentrale Rolle der Methodenentwicklung in der gerichteten Evolution selektiver Enzyme

An effective hybrid strategy for converting rice straw to furoic acid by tandem catalysis via Sn-sepiolite combined with recombinant E. coli whole cells harboring horse liver alcohol dehydrogenase

Rapid and Error-Free Site-Directed Mutagenesis by a PCR-Free In Vitro CRISPR/Cas9-Mediated Mutagenic System

Back Cover: Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase (Angew. Chem. Int. Ed. 3/2019)

Rücktitelbild: Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase (Angew. Chem. 3/2019)

Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase

Solid‐Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution?

Enhancing the Catalytic Performance of a CYP116B Monooxygenase by Transdomain Combination Mutagenesis

Front Cover: Enhancing the Catalytic Performance of a CYP116B Monooxygenase by Transdomain Combination Mutagenesis (ChemCatChem 14/2018)

Boosting the efficiency of site-saturation mutagenesis for a difficult-to-randomize gene by a two-step PCR strategy

P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping

Bioamination of alkane with ammonium by an artificially designed multienzyme cascade