王连荣｜武汉大学｜学者主页

2026

Dual cytosine modification through hydroxylation and arabinofuranosylation in phages

Yu He, Haiyan Gao, Yudong Yang, Dong-Won Yang, Lixu Jiang, Junwu Bi, Fuliang Yin, Yaru Tang, Zheng Zhao, Shi Chen, Lian-Rong Wang

Nature Chemical Biology

DOI

2026

A novel putative genus phage CW39: implications for CRISPR-Cas9-based phage resistance in Streptomyces avermitilis

Chang Wen, Yingying Wang, Lian-Rong Wang, Shi Chen

Frontiers in Microbiology

DOI

2026

SspE-mediated immune defense: GTP hydrolysis as an allosteric switch coupling phosphorothioate recognition to DNA cleavage

Yu-Feng Zhou, Kuo Zhang, Yu He, Haiyan Gao, Yuhang Zhong, Xiao-Guang Wang, Mei-Ying Wang, Lian-Rong Wang, Shi Chen

mBio

DOI

2026

Phage hijacks host phosphorothioate DNA modification machinery to circumvent bacterial Ssp defences

Yifei Wang, Hao-Yi Yang, Lixu Jiang, Yashi Ge, Yuhang Zhong, Hui Chu, Kuo Zhang, Kadierya Kuerban, Yuan Nong, Haiyan Gao, Congrui Xu, Xiaoyu Li, Shi Chen, Lian-Rong Wang

Nature Microbiology

DOI

2025

DNA硫修饰基础理论突破驱动合成生物学系统创新

帅玉婷，韩照晰，贺新义，王连荣，陈实，邓子新，刘光

《生物工程学报》

DOI

2025

A DNA phosphorothioation pathway via adenylated intermediate modulates Tdp machinery

Tianchen An, Qian Tan, Lixu Jiang, Li Liu, Xing Yu Jiang, Liying Liu, Xiaofei Chang, Xihao Tian, Zixin Deng, Shuai Gao, Lianrong Wang, Shi Chen

Nature Chemical Biology

DOI

2025

Mechanistic insights into JSS1_004-mediated antagonism of the DndBCDE-FGH restriction system and engineering applications

Yu He, Susu Jiang, Fang Wang, Junwu Bi, Shi-Pian Li, Fuliang Yin, Shi Chen, Chao Chen, Lianrong Wang

mBio

DOI

2025

Molecular Insight into the Recognition of DNA by the DndCDE Complex in DNA Phosphorothioation

Wencheng Fu, Yuli Wang, Yashi Ge, Haiyan Gao, Xuan Sun, Zixin Deng, Lianrong Wang, Shi Chen, Xinyi He, Geng Wu

International Journal of Molecular Sciences

DOI

2025

In silico approaches for discovering microbial antiviral defense systems

Lixu Jiang, Yansheng Li, Baocai Xie, Lian-Rong Wang, Shi Chen

Briefings in Bioinformatics

DOI

2025

The Ppl protein senses 3'-hydroxyl DNA overhangs and NTP depletion to halt phage infection.

Zixiao Xu, Hong-Qiu Pu, Lixu Jiang, Zhi-Ming Wang, K. Kong, Lian-Rong Wang, Shi Chen

Molecules and Cells

DOI

2024

Molecular basis of the phosphorothioation-sensing antiphage defense system IscS–DndBCDE–DndI

Yaqian Tang, Dan Wu, Yueying Zhang, Xuan Liu, Hui Chu, Qian Tan, Lixu Jiang, Shi Chen, Geng Wu, Lianrong Wang

Nucleic Acids Research

DOI

2024

DNA Phosphorothioate Modification Systems and Associated Phage Defense Systems

Lianrong Wang, Yaqian Tang, Zixin Deng, Shi Chen

Annual Review of Microbiology

DOI

2023

A DNA phosphorothioation-based Dnd defense system provides resistance against various phages and is compatible with the Ssp defense system

Susu Jiang, Ke Chen, Yingying Wang, Yueying Zhang, Yaru Tang, Wanqiu Huang, Xiaolin Xiong, Shi Chen, Chao Chen, Lianrong Wang

mBio

DOI

2023

The DNA Phosphorothioation Restriction-Modification System Influences the Antimicrobial Resistance of Pathogenic Bacteria

Congrui Xu, Jing Rao, Yuqing Xie, Jiajun Lu, Zhiqiang Li, Changjiang Dong, Lianrong Wang, Jinghong Jiang, Chao Chen, Shi Chen

Microbiology Spectrum

DOI

2022

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

Haiyan Gao, Xinqi Gong, Jinchuan Zhou, Yubing Zhang, Jinsong Duan, Yue Wei, Liuqing Chen, Zixin Deng, Jiawei Wang, Shi Chen, Geng Wu, Lianrong Wang

Nature Communications

DOI

2022

The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex

Dan Wu, Yaqian Tang, Siwei Chen, Yue He, Yue He, Xiaofei Chang, Wenzhong Zheng, Zixin Deng, Zhiqiang Li, Lianrong Wang, Geng Wu, Shi Chen, Shi Chen

Nature Catalysis

DOI

2022

Structural and Functional Analysis of DndE Involved in DNA Phosphorothioation in the Haloalkaliphilic Archaea Natronorubrum bangense JCM10635

Wei He, Haiyan Gao, Dan Wu, Susu Jiang, Wanqiu Huang, Chao Chen, Zixin Deng, Lei Xiong, Geng Wu, Lianrong Wang

mBio

DOI

2022

Involvement of the DNA Phosphorothioation System in TorR Binding and Anaerobic TMAO Respiration in Salmonella enterica

You Tang, Yue Hong, Li Liu, Xuan Du, Yaqi Ren, Susu Jiang, Wanjing Liu, Chao Chen, Zixin Deng, Lianrong Wang, Shi Chen

mBio

DOI

2021

The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes

Huahua Jian, Guanpeng Xu, Yi Yi, Yali Hao, Yinzhao Wang, Lei Xiong, Siyuan Wang, Shunzhang Liu, Canxing Meng, Jiahua Wang, Yue Zhang, Chao Chen, Xiaoyuan Feng, Haiwei Luo, Hao Zhang, Xingguo Zhang, Lianrong Wang, Zhijun Wang, Zixin Deng, Xiang Xiao

Nature Communications

DOI

2021

Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics

Yue Wei, Qinqin Huang, Xihao Tian, Mingmin Zhang, Junkai He, Xingxiang Chen, Chao Chen, Zixin Deng, Zhiqiang Li, Shi Chen, Lianrong Wang

Nucleic Acids Research

DOI

2021

SspABCD-SspFGH Constitutes a New Type of DNA Phosphorothioate-Based Bacterial Defense System

Shiwei Wang, Mengping Wan, Ruolin Huang, Yujing Zhang, Yuqing Xie, Yue Wei, Mustafa Ahmad, Dan Wu, Yue Hong, Zixin Deng, Shi Chen, Zhiqiang Li, Lianrong Wang

mBio

DOI

2020

Epigenetic competition reveals density-dependent regulation and target site plasticity of phosphorothioate epigenetics in bacteria

Xiaolin Wu, Bo Cao, Patricia Aquino, Tsu-Pei Chiu, Chao Chen, Susu Jiang, Zixin Deng, Shi Chen, Remo Rohs, Lianrong Wang, James E. Galagan, Peter C. Dedon

Proceedings of the National Academy of Sciences

DOI

2020

Structural Analysis of an l -Cysteine Desulfurase from an Ssp DNA Phosphorothioation System

Liqiong Liu, Susu Jiang, Xing Mai, Chao Chen, Chongde Lai, Na Li, Guangfeng Liu, Dan Wu, Haiyan Gao, Liang Hong, Pan Tan, Shi Chen, Zixin Deng, Geng Wu, Lianrong Wang

mBio

DOI

2020

SspABCD–SspE is a phosphorothioation-sensing bacterial defence system with broad anti-phage activities

Xiaolin Xiong, Geng Wu, Yue Wei, Liqiong Liu, Yubing Zhang, Rui Su, Xianyue Jiang, Mengxue Li, Haiyan Gao, Xihao Tian, Yizhou Zhang, Li Hu, Si Chen, You Tang, Susu Jiang, Ruolin Huang, Zhiqiang Li, Yunfu Wang, Zixin Deng, Jiawei Wang, Peter C. Dedon, Shi Chen, Lianrong Wang

Nature Microbiology

DOI

2019

A new type of DNA phosphorothioation-based antiviral system in archaea

Lei Xiong, Siyi Liu, Si Chen, Si Chen, Yao Xiao, Bochen Zhu, Yali Gao, Yujing Zhang, Beibei Chen, Jie Luo, Zixin Deng, Xiangdong Chen, Lianrong Wang, Shi Chen, Shi Chen

Nature Communications

DOI

2018

肠道微生物组与宿主的表观遗传修饰

熊智，王连荣，陈实

《微生物学报》

2018

Tight control of genomic phosphorothioate modification by the ATP‐modulated autoregulation and reusability of DndB

Sisi Xia, Jun Chen, Liqiong Liu, Yue Wei, Zixin Deng, Lianrong Wang, Shi Chen

Molecular Microbiology

DOI

2018

DNA phosphorothioate modification—a new multi-functional epigenetic system in bacteria

Lianrong Wang, Susu Jiang, Zixin Deng, Peter C. Dedon, Shi Chen

FEMS Microbiology Reviews

DOI

2018

Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria

Tong Tong, Si Chen, Lianrong Wang, You Tang, Jae Yong Ryu, Susu Jiang, Xiaolin Wu, Chao Chen, Jie Luo, Zixin Deng, Zhiqiang Li, Sang Yup Lee, Shi Chen

Proceedings of the National Academy of Sciences

DOI

2017

甲基化修饰在细菌表观调控中的功能

童童，王连荣

《微生物学报》

2017

Convergence of DNA methylation and phosphorothioation epigenetics in bacterial genomes

Chao Chen, Lianrong Wang, Si Chen, Xiaolin Wu, Meijia Gu, Xi Chen, Susu Jiang, Yunfu Wang, Zixin Deng, Peter C. Dedon, Shi Chen

Proceedings of the National Academy of Sciences

DOI

2015

微生物药物合成的放线菌底盘

池淏甜，王连荣

《生物产业技术》

2015

Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB

Wei He, Teng Huang, You Tang, Yanhua Liu, Xiaolin Wu, Si Chen, Wan Chan, Yajie Wang, Xiaoyun Liu, Shi Chen, Lianrong Wang

Scientific Reports

DOI

2014

Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences

Bo Cao, Chao Chen, Michael S. DeMott, Qiuxiang Cheng, Tyson A. Clark, Xiaolin Xiong, Xiaoqing Zheng, Vincent L. Butty, Stuart S. Levine, George Yuan, Matthew Boitano, Khai Luong, Yi Song, Xiufen Zhou, Zixin Deng, Stephen W. Turner, Jonas Korlach, Delin You, Lianrong Wang, Shi Chen, Peter C. Dedon

Nature Communications

DOI

2014

DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks

Rui Gan, Xiaolin Wu, Wei He, Zhenhua Liu, Shuangju Wu, Chao Chen, Si Chen, Qianrong Xiang, Zixin Deng, Dequan Liang, Shi Chen, Lianrong Wang

Scientific Reports

DOI

2014

In Vivo Mutational Characterization of DndE Involved in DNA Phosphorothioate Modification

Chongde Lai, Xiaolin Wu, Chao Chen, Teng Huang, Xiaolin Xiong, Shuangju Wu, Meijia Gu, Zixin Deng, Xi Chen, Shi Chen, Lianrong Wang

PLoS ONE

DOI

2013

Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences

RePEc: Research Papers in Economics

2011

DNA phosphorothioation is widespread and quantized in bacterial genomes

Lianrong Wang, Shi Chen, Kevin L. Vergin, Stephen J. Giovannoni, Simon W. Chan, Michael S. DeMott, Koli Taghizadeh, Otto X. Cordero, Michael Cutler, Sonia C. Timberlake, Eric J. Alm, Martin F. Polz, Jarone Pinhassi, Zixin Deng, Peter C. Dedon

Proceedings of the National Academy of Sciences

DOI

2010

Twenty years hunting for sulfur in DNA

Shi Chen, Lian-Rong Wang, Zi-Xin Deng

Protein & Cell

DOI

2009

Young investigator: Lianrong Wang.

Lian-Rong Wang, K. Taghizadeh

Bioanalysis

DOI

2007

Phosphorothioation of DNA in bacteria by dnd genes.

Lian-Rong Wang, Shi Chen, Tie-Gang Xu, K. Taghizadeh, J. Wishnok, Xiufen Zhou, De-Lin You, Zi-Xin Deng, P. Dedon

Nature Chemical Biology

DOI

2007

A novel DNA modification by sulfur: DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans.

De-Lin You, Lian-Rong Wang, Fen Yao, Xiufen Zhou, Zi-Xin Deng

Biochemistry

DOI

精选作品

期刊论文

Dual cytosine modification through hydroxylation and arabinofuranosylation in phages

A novel putative genus phage CW39: implications for CRISPR-Cas9-based phage resistance in Streptomyces avermitilis

SspE-mediated immune defense: GTP hydrolysis as an allosteric switch coupling phosphorothioate recognition to DNA cleavage

Phage hijacks host phosphorothioate DNA modification machinery to circumvent bacterial Ssp defences

DNA硫修饰基础理论突破驱动合成生物学系统创新

A DNA phosphorothioation pathway via adenylated intermediate modulates Tdp machinery

Mechanistic insights into JSS1_004-mediated antagonism of the DndBCDE-FGH restriction system and engineering applications

Molecular Insight into the Recognition of DNA by the DndCDE Complex in DNA Phosphorothioation

In silico approaches for discovering microbial antiviral defense systems

The Ppl protein senses 3'-hydroxyl DNA overhangs and NTP depletion to halt phage infection.

Molecular basis of the phosphorothioation-sensing antiphage defense system IscS–DndBCDE–DndI

DNA Phosphorothioate Modification Systems and Associated Phage Defense Systems

A DNA phosphorothioation-based Dnd defense system provides resistance against various phages and is compatible with the Ssp defense system

The DNA Phosphorothioation Restriction-Modification System Influences the Antimicrobial Resistance of Pathogenic Bacteria

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex

Structural and Functional Analysis of DndE Involved in DNA Phosphorothioation in the Haloalkaliphilic Archaea Natronorubrum bangense JCM10635

Involvement of the DNA Phosphorothioation System in TorR Binding and Anaerobic TMAO Respiration in Salmonella enterica

The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotes

Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics

SspABCD-SspFGH Constitutes a New Type of DNA Phosphorothioate-Based Bacterial Defense System

Epigenetic competition reveals density-dependent regulation and target site plasticity of phosphorothioate epigenetics in bacteria

Structural Analysis of an l -Cysteine Desulfurase from an Ssp DNA Phosphorothioation System

SspABCD–SspE is a phosphorothioation-sensing bacterial defence system with broad anti-phage activities

A new type of DNA phosphorothioation-based antiviral system in archaea

肠道微生物组与宿主的表观遗传修饰

Tight control of genomic phosphorothioate modification by the ATP‐modulated autoregulation and reusability of DndB

DNA phosphorothioate modification—a new multi-functional epigenetic system in bacteria

Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria

甲基化修饰在细菌表观调控中的功能

Convergence of DNA methylation and phosphorothioation epigenetics in bacterial genomes

微生物药物合成的放线菌底盘

Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB

Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences

DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks

In Vivo Mutational Characterization of DndE Involved in DNA Phosphorothioate Modification

Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences

DNA phosphorothioation is widespread and quantized in bacterial genomes

Twenty years hunting for sulfur in DNA

Young investigator: Lianrong Wang.

Phosphorothioation of DNA in bacteria by dnd genes.

A novel DNA modification by sulfur: DndA is a NifS-like cysteine desulfurase capable of assembling DndC as an iron-sulfur cluster protein in Streptomyces lividans.

书籍章节

Phosphorothioation: An Unusual Post-Replicative Modification on the DNA Backbone

研究方向