杨世辉｜湖北大学｜学者主页

2026

合成生物学赋能微生物细胞疗法：智能设计与精准治疗

任贻群，王振，何桥宁，杨世辉

《中国生物工程杂志》

2026

Synthesis, characteristics, and applications of sustainable carbon quantum dots derived from biomass of recombinant bacterium

Zhen Wang, Qiaoning He, Boyi Deng, Ludan He, Mimi Hu, Yifan Wang, Jianbing Zhang, Shihui Yang

Bioresource Technology

DOI

2026

Development of robust dominant-metabolism compromised intermediate chassis of ethanologenic Saccharomyces cerevisiae for non-ethanol biochemical production

Qi Zhou, Xiongying Yan, Yalun Wu, Qiaoning He, Shihui Yang

Bioresource Technology

DOI

2026

Biological valorization of methane and nitrogen gas-derived ammonia via methanotrophic bacteria for gut-beneficial nutrients

Zixi Gao, Yuan Liu, Shanyao Jiao, Rongzhan Fu, Yunhao Chen, Chenyue Zhang, Xuemei Shen, Guidong Yang, Shihui Yang, Xiaoyan Wang, Liang Shi, Qiang Fei

Nature Communications

DOI

2026

S267P mutation of OxyR regulator in Zymomonas mobilis: mechanism of oxidative stress tolerance and applications in cellulosic hydrolysate fermentation and oxidative stress monitoring

Yi Wang, Binan Geng, Shuyi Liu, Yalun Wu, Shihui Yang

Synthetic and Systems Biotechnology

DOI

2026

A synergistic strategy of metabolic engineering and flocculation recycling for enhanced acetoin production in Zymomonas mobilis

Qiqun Peng, Qiqun Peng, Hongxiang Yu, Hongwei Yang, Shihui Yang

Synthetic and Systems Biotechnology

DOI

2026

Engineering ethanologenic bacterium Zymomonas mobilis for economic cellulosic bioethanol production using agro-industrial waste corncob residue

Yongfu Yang, Chenyue Zhang, Binan Geng, Ying Tang, Haixia Yi, Jun Du, Qiang Fei, Shihui Yang

Synthetic and Systems Biotechnology

DOI

2025

合成生物学竞赛：驱动产学研融合的新引擎

晏雄鹰，王依，耿碧男，何桥宁，杨世辉

《合成生物学》

2025

多技术融合与原料革新驱动生物制造发展

晏雄鹰，耿碧男，李长征，胡蜜蜜，何桥宁，杨世辉

《生物工程学报》

DOI

2025

Engineering strategies and tolerance mechanisms for robust d-lactate producer development of Zymomonas mobilis

Mimi Hu, Yunhao Chen, Binan Geng, Xiongying Yan, Yalun Wu, Qiqun Peng, Jihong Chen, Wei Hu, Qiaoning He, Shihui Yang

Bioresource Technology

DOI

2025

AI-powered high-throughput digital colony picker platform for sorting microbial strains by multi-modal phenotypes

Zhidian Diao, Qiqun Peng, Sijun Luo, Lingyan Kan, Anle Ge, Wei Gao, Runxia Li, Weiwei Bao, Xixian Wang, Yuetong Ji, Jian Xu, Shihui Yang, Bo Ma

Nature Communications

DOI

2025

Establishment of a Visible Reporter System in Zymomonas mobilis through Random Mutagenesis and Rational Design of a Chromoprotein eforRed

Xinyu Yang, Ning Yang, Siqi Wu, Shuche He, Wei Jiang, WeiXuan Zhong, Jun Cheng Du, Guimin Zhang, Xia Wang, Shihui Yang

ACS Synthetic Biology

DOI

2025

Establishment of a RecET-Assisted CRISPR-Cas12a System for Large Deoxyribonucleic Acid-Fragment Manipulation in Zymomonas mobilis

Yujie Zhang, Jian Liu, Yunfeng Zhou, Xiaoyue Hu, Binan Geng, Shihui Yang

ACS Synthetic Biology

DOI

2025

A dual-tag strategy with superfolder green fluorescent protein (sfGFP) and small ubiquitin-like modifier (SUMO) for soluble expression of SARS-CoV-2 RNA polymerase visibly in Escherichia coli

Haoyue Song, Fei Wang, Wenli Xie, Zhizhao Chen, Ruyi He, Yu Fang, Qiaoning He, Shihui Yang, Lixin Ma

International Journal of Biological Macromolecules

DOI

2025

Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production

Jiyun Lou, Xia Wang, Runxia Li, Jun Yao, Junpeng Hu, Xianwu Qin, Mingjie Jin, Shihui Yang

Synthetic and Systems Biotechnology

DOI

2025

Metabolic Engineering of Zymomonas mobilis for Xylonic Acid Production from Lignocellulosic Hydrolysate

Banrui Ruan, Xiongying Yan, Zhaoqing He, Qiaoning He, Shihui Yang

Fermentation

DOI

2025

Metabolic Engineering of Escherichia coli for Xylitol Production

Jiapeng Li, Lei Zhang, Changzheng Li, Zhaoqing He, Xiongying Yan, Shihui Yang

Fermentation

DOI

2025

Transcriptome analysis and reverse engineering verification of SNZ3Val125Ile and Pho3Asn134Asp revealed the mechanism of adaptive laboratory evolution to increase the yield of tyrosol in Saccharomyces cerevisiae strain S26-AE2

Na Song, Huili Xia, Xiaoxue Yang, Siyao Liu, Linlong Xu, Kun Zhuang, Lan Yao, Shihui Yang, Xiong Chen, Jun Dai

Biotechnology for Biofuels and Bioproducts

DOI

2024

Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis

Xiongying Yan, Weiwei Bao, Yalun Wu, Chenyue Zhang, Zhitao Mao, Qianqian Yuan, Zhousheng Hu, Penghui He, Qiqun Peng, Mimi Hu, Binan Geng, Hongwu Ma, Shouwen Chen, Qiang Fei, Qiaoning He, Shihui Yang

Nature Communications

DOI

2024

Systematic metabolic engineering of Zymomonas mobilis for β-farnesene production

Yubei Xiao, Xuemei Tan, Qiaoning He, Shihui Yang

Frontiers in Bioengineering and Biotechnology

DOI

2024

Systematic investigation of TetR-family transcriptional regulators and their roles on lignocellulosic inhibitor acetate tolerance in Zymomonas mobilis

Yubei Xiao, Tongjia Qin, Shuche He, Yunhao Chen, Han Li, Qiaoning He, Xia Wang, Shihui Yang

Frontiers in Bioengineering and Biotechnology

DOI

2024

Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production

Qiqun Peng, Weiwei Bao, Binan Geng, Shihui Yang

Synthetic and Systems Biotechnology

DOI

2024

A novel nutritional induction strategy flexibly switching the biosynthesis of food-like products from methane by a methanotrophic bacterium

Zixi Gao, Shuqi Guo, Yunhao Chen, Hansen Chen, Rongzhan Fu, Qiaoqiao Song, Shen Li, Wen‐Yong Lou, Daidi Fan, Yin Li, Shihui Yang, Ramón González, Qiang Fei

Green Chemistry

DOI

2024

Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1 F427L mutation in the presence of glycerol

Zixiong Liu, Lingling Shangguan, Linlong Xu, Huiyan Zhang, Wenxin Wang, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Xiong Chen, Jun Dai

Microbiology Spectrum

DOI

2024

Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol in Saccharomyces cerevisiae

Na Song, Huili Xia, Yaoru Xie, Shuaikang Guo, Rong Zhou, Lingling Shangguan, Kun Zhuang, Huiyan Zhang, Feiran An, Xueyun Zheng, Lan Yao, Shihui Yang, Xiong Chen, Jun Dai

Synthetic and Systems Biotechnology

DOI

2024

Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis

Binan Geng, Yalun Wu, Wu Xinyan, Yongfu Yang, Zhou Peng, Yunhao Chen, Zhou Xuan, Chen‐Guang Liu, Feng‐Wu Bai, Ping Xu, Qiaoning He, Shihui Yang

Trends in biotechnology

DOI

2024

Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production

Zhen Wang, Xia Wang, Xiongying Yan, Haixia Yi, Shuche He, Haoyu Zhang, Xinli Zhou, Qiaoning He, Shihui Yang

Synthetic and Systems Biotechnology

DOI

2024

Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation

Xiongying Yan, Qiaoning He, Binan Geng, Shihui Yang

BioDesign Research

DOI

2023

生物燃料高效生产微生物细胞工厂构建研究进展

晏雄鹰，王振，娄吉芸，张皓瑜，黄星宇，王霞，杨世辉

《合成生物学》

2023

微生物硫代谢与抗逆性

晏雄鹰，王振，王霞，杨世辉

《生物技术通报》

2023

Adaptive Laboratory Evolution and Metabolic Engineering of Zymomonas mobilis for Bioethanol Production Using Molasses

Ju Wen Huang, Xia Wang, Xiangyu Chen, Han Li, Yunhao Chen, Zhousheng Hu, Shihui Yang

ACS Synthetic Biology

DOI

2023

In vitro characterization of a pAgo nuclease TtdAgo from Thermococcus thioreducens and evaluation of its effect in vivo

Ying Tang, Fei Wang, Yi Wang, Yuwei Wang, Yang Liu, Zhizhao Chen, Wenqiang Li, Shihui Yang, Lixin Ma

Frontiers in Bioengineering and Biotechnology

DOI

2023

Metabolic engineering of Zymomonas mobilis for co-production of D-lactic acid and ethanol using waste feedstocks of molasses and corncob residue hydrolysate

Mimi Hu, Weiwei Bao, Qiqun Peng, Wei Hu, Xinyu Yang, Xiang Yu Yan, Xiongying Yan, Mian Li, Ping Xu, Qiaoning He, Shihui Yang

Frontiers in Bioengineering and Biotechnology

DOI

2023

Regulation of biofilm formation in Zymomonas mobilis to enhance stress tolerance by heterologous expression of pfs and luxS

Lian-Ying Cao, Chen‐Guang Liu, Shihui Yang, Feng‐Wu Bai

Frontiers in Bioengineering and Biotechnology

DOI

2023

Systematic metabolic engineering of Bacillus licheniformis for hyperproduction of the antioxidant hydroxytyrosol

Yangyang Zhan, Fei Zhou, Wenqi Ruan, Hao Yin, Zhi Li, Huan Wang, Li Tao, Dongbo Cai, Shihui Yang, Xin Ma, Shouwen Chen

Green Chemistry

DOI

2023

Isolation and Identification of Non-Saccharomyces Yeast Producing 2-Phenylethanol and Study of the Ehrlich Pathway and Shikimate Pathway

Rong Zhou, Qingyi Song, Huili Xia, Na Song, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Jun Dai, Xiong Chen

Journal of Fungi

DOI

2023

Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories

Yalun Wu, Qianqian Yuan, Yongfu Yang, Defei Liu, Shihui Yang, Hongwu Ma

Synthetic and Systems Biotechnology

DOI

2023

Improved Glutamic Acid Production Capacity of Corynebacterium glutamicum by the ARTP Mutagenesis Method

Lingling Shangguan, Huiyan Zhang, Zixiong Liu, Feiran An, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Jun Dai, Xiong Chen

Fermentation

DOI

2023

CAVE: a cloud-based platform for analysis and visualization of metabolic pathways

Zhitao Mao, Qianqian Yuan, Haoran Li, Yue Zhang, Yuanyuan Huang, Chunhe Yang, Ruoyu Wang, Yongfu Yang, Yalun Wu, Shihui Yang, Xiaoping Liao, Hongwu Ma

Nucleic Acids Research

DOI

2023

Metabolic Engineering of Zymomonas mobilis for Acetoin Production by Carbon Redistribution and Cofactor Balance

Weiwei Bao, Wei Shen, Qiqun Peng, Jun Cheng Du, Shihui Yang

Fermentation

DOI

2023

Synchronous efforts for burgeoning bioeconomy: The 4th international biodesign research conference and international symposium on development and application of modern biotechnology

Qiaoning He, Huimin Yu, Shihui Yang

Biotechnology Notes

DOI

2022

拉曼光谱分析技术在资源微生物发酵性能评价中的应用

鲍伟威，纪凯迪，胡蜜蜜，娄吉芸，彭祺群，李震，王霞，阮银兰，杨世辉

《微生物学报》

2022

Synthetic refactor of essential genes decodes functionally constrained sequences in yeast genome

Zhenzhen Liang, Zhouqing Luo, Weimin Zhang, Yu Kang, Hui Wang, Binan Geng, Qing Yang, Zuoyu Ni, Cheng Zeng, Yihui Zheng, Chunyuan Li, Shihui Yang, Yingxin Ma, Junbiao Dai

iScience

DOI

2022

Evolutionary and reverse engineering in Saccharomyces cerevisiae reveals a Pdr1p mutation-dependent mechanism for 2-phenylethanol tolerance

Huili Xia, Yue Kang, Zilin Ma, Cuiyu Hu, Qiao Yang, Xiaoling Zhang, Shihui Yang, Jun Dai, Xiong Chen

Microbial Cell Factories

DOI

2022

A plasmid-free Zymomonas mobilis mutant strain reducing reactive oxygen species for efficient bioethanol production using industrial effluent of xylose mother liquor

Binan Geng, Shuyi Liu, Yunhao Chen, Yalun Wu, Yi Wang, Xuan Zhou, Han Li, Mian Li, Shihui Yang

Frontiers in Bioengineering and Biotechnology

DOI

2022

Molecular mechanism of enhanced ethanol tolerance associated with hfq overexpression in Zymomonas mobilis

Ying Tang, Yi Wang, Qing Yang, Youpeng Zhang, Yalun Wu, Yongfu Yang, Meng Mei, Mingxiong He, Xia Wang, Shihui Yang

Frontiers in Bioengineering and Biotechnology

DOI

2022

Rapamycin enhanced the production of 2-phenylethanol during whole-cell bioconversion by yeast

Huili Xia, Lingling Shangguan, Sheng Chen, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Jun Dai, Xiong Chen

Applied Microbiology and Biotechnology

DOI

2022

Determination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between Zymomonas mobilis and Escherichia coli

Haoyue Song, Yongfu Yang, Han Li, Jun Cheng Du, Zhousheng Hu, Yunhao Chen, Ning Yang, Meng Mei, Zhiqiang Xiong, Ke Tang, Yi Li, Yu Zhang, Shihui Yang

ACS Synthetic Biology

DOI

2022

Transcriptomic profiling of nitrogen fixation and the role of NifA in Methylomicrobium buryatense 5GB1

Shuqi Guo, Tianqing Zhang, Yunhao Chen, Shihui Yang, Qiang Fei

Applied Microbiology and Biotechnology

DOI

2022

Cysteine supplementation enhanced inhibitor tolerance of Zymomonas mobilis for economic lignocellulosic bioethanol production

Xiongying Yan, Xia Wang, Yongfu Yang, Zhen Wang, Haoyu Zhang, Yang Li, Qiaoning He, Mian Li, Shihui Yang

Bioresource Technology

DOI

2022

Multilevel metabolic engineering of Bacillus licheniformis for de novo biosynthesis of 2-phenylethanol

Yangyang Zhan, Jiao Shi, Yuan Xiao, Fei Zhou, Huan Wang, Haixia Xu, Zhi Li, Shihui Yang, Dongbo Cai, Shouwen Chen

Metabolic Engineering

DOI

2022

Metabolic engineering of Zymomonas mobilis for continuous co-production of bioethanol and poly-3-hydroxybutyrate (PHB)

Yang Li, Ying Wang, Ruxiang Wang, Xiongying Yan, Jiewen Wang, Xia Wang, Shouwen Chen, Feng‐Wu Bai, Qiaoning He, Shihui Yang

Green Chemistry

DOI

2022

The potential use of Zymomonas mobilis for the food industry

Lu Liu, Jian-Ting Li, Sheng-Hao Li, Lin-Pei Liu, Bo Wu, Yanwei Wang, Shihui Yang, Cheng‐Han Chen, Furong Tan, Mingxiong He

Critical Reviews in Food Science and Nutrition

DOI

2022

Identification and Characterization of Genes Related to Ampicillin Antibiotic Resistance in Zymomonas mobilis

Binan Geng, Xingyu Huang, Yalun Wu, Qiaoning He, Shihui Yang

Antibiotics

DOI

2022

Benchmarking of long-read sequencing, assemblers and polishers for yeast genome

Xue Zhang, Chen‐Guang Liu, Shihui Yang, Xia Wang, Feng‐Wu Bai, Zhuo Wang

Briefings in Bioinformatics

DOI

2022

Ethanol production from lignocellulosic biomass by co-fermentation with Pecoramyces sp. F1 and Zymomonas mobilis ATCC 31821 in an integrated process

Yuqi Li, Yao Xu, Yihan Xue, Shihui Yang, Yanfen Cheng, Weiyun Zhu

Biomass and Bioenergy

DOI

2022

Deciphering Molecular Mechanism Underlying Self-Flocculation of Zymomonas mobilis for Robust Production

Lian-Ying Cao, Yongfu Yang, Xue Zhang, Yunhao Chen, Ji-Wen Yao, Xia Wang, Juan Xia, Chen‐Guang Liu, Shihui Yang, Ute Römling, Feng‐Wu Bai

Applied and Environmental Microbiology

DOI

2022

Global reprogramming of xylose metabolism in Saccharomyces cerevisiae efficiently produces ethanol from lignocellulose hydrolysates

Xiaowei Li, Rui Xiang Zhao, Shan Li, Yating Wang, Xinnan Wang, Wenya Yang, Mengchan Yang, Weihua Xiao, Shihui Yang, Xiaoqing Lin, Xiaojie Zheng, Xiaojun Ma, Ling Zhao, Wei Xiao, Limin Cao

Industrial Crops and Products

DOI

2022

Development of a Hyperthermostable Artificial Scaffold Based on Ultrahigh-Affinity Protein Pairs and Its Application in Cellulose Degradation

Jun Hyeok Yang, Fei Wang, Shihui Yang, Wenxue Jiang, Rong Gao, Siyu Lin, Binyang Deng, Xiaoyu Wang, Wei Liang Cheng, Yang Liu, Chin‐Yu Chen, Qiong Xing, Chao Zhai, Lixin Ma

ACS Sustainable Chemistry & Engineering

DOI

2022

Differential analysis of ergosterol function in response to high salt and sugar stress in Zygosaccharomyces rouxii

Na Song, Huili Xia, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Xiong Chen, Jun Dai

FEMS Yeast Research

DOI

2022

Double nicking by RNA-directed Cascade-nCas3 for high-efficiency large-scale genome engineering

Yile Hao, Qinhua Wang, Jie Li, Shihui Yang, Lixin Ma, Yanli Zheng, Wenfang Peng

Open Biology

DOI

2021

运动发酵单胞菌底盘细胞研究现状及展望

杨永富，耿碧男，宋皓月，何桥宁，何明雄，鲍杰，白凤武，杨世辉

《合成生物学》

2021

合成生物学时代基于非模式细菌的工业底盘细胞研究现状与展望

杨永富，耿碧男，宋皓月，胡蜜蜜，何桥宁，陈守文，白凤武，杨世辉

《生物工程学报》

DOI

2021

Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis

Xin Sui, Xiaojie Wang, Tao Liu, Qing Ye, Bo Wu, Guoquan Hu, Shihui Yang, Mingxiong He, Nan Peng

Biotechnology for Biofuels

DOI

2021

Synthetic counter-selection markers and their application in genetic modification of Synechococcus elongatus UTEX2973

Liyuan Chen, Hai Liu, Lı Wang, Xiaoming Tan, Shihui Yang

Applied Microbiology and Biotechnology

DOI

2021

Metabolic Engineering of Bacillus licheniformis for Sustainable Production of Isobutanol

Yangyang Zhan, Yong Quan Xu, Xingcheng Lu, Fei Zhou, Pengling Zheng, Dong Wang, Dongbo Cai, Shihui Yang, Shouwen Chen

ACS Sustainable Chemistry & Engineering

DOI

2021

Revitalizing the ethanologenic bacterium Zymomonas mobilis for sugar reduction in high-sugar-content fruits and commercial products

Mimi Hu, Xiangyu Chen, Ju Wen Huang, Jun Cheng Du, Mian Li, Shihui Yang

Bioresources and Bioprocessing

DOI

2021

Development and characterization of efficient xylose utilization strains of Zymomonas mobilis

Jiyun Lou, Jingwen Wang, Yongfu Yang, Qing Yang, Runxia Li, Mimi Hu, Qiaoning He, Jun Cheng Du, Xia Wang, Mian Li, Shihui Yang

Biotechnology for Biofuels

DOI

2021

Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis

Lingling Sun, Bo Wu, Zengqin Zhang, Jing‐Kun Yan, Panting Liu, Chao Song, Samina Shabbir, Qili Zhu, Shihui Yang, Nan Peng, Mingxiong He, Furong Tan

Biotechnology for Biofuels

DOI

2021

A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Shengchen Wang, Faying Zhang, Meng Mei, Ting Wang, Yueli Yun, Shihui Yang, Guimin Zhang, Yi Li

Communications Biology

DOI

2021

Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

Runxia Li, Wei Shen, Yongfu Yang, Jun Cheng Du, Mian Li, Shihui Yang

Biotechnology for Biofuels

DOI

2021

Year-Round Storage Operation of Three Major Agricultural Crop Residue Biomasses by Performing Dry Acid Pretreatment at Regional Collection Depots

Tao Han, Bin Zhang, Hongxing Li, Hongsen Zhang, Yongfu Yang, Lizhen Hu, Xiaodong Ren, Shipeng Wang, Lixiang Zheng, Xushen Han, Gang Liu, Jian Zhang, Qiang Fei, Yue‐Qin Tang, Shihui Yang, Xiaoming Bao, Jie Bao

ACS Sustainable Chemistry & Engineering

DOI

2020

The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance of Zymomonas mobilis

Runxia Li, Mingjie Jin, Jun Du, Mian Li, Shouwen Chen, Shihui Yang

Frontiers in Bioengineering and Biotechnology

DOI

2020

Origins and features of pectate lyases and their applications in industry

Pan Wu, Shihui Yang, Zhichun Zhan, Guimin Zhang

Applied Microbiology and Biotechnology

DOI

2020

Molecular Mechanism Associated With the Impact of Methane/Oxygen Gas Supply Ratios on Cell Growth of Methylomicrobium buryatense 5GB1 Through RNA-Seq

Lizhen Hu, Yongfu Yang, Xin Yan, Tianqing Zhang, Jing Xiang, Zixi Gao, Yunhao Chen, Shihui Yang, Qiang Fei

Frontiers in Bioengineering and Biotechnology

DOI

2020

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering

Yanli Zheng, Jie Li, Baiyang Wang, Jiamei Han, Yile Hao, Shengchen Wang, Xiangdong Ma, Shihui Yang, Lixin Ma, Yi Li, Wenfang Peng

Frontiers in Bioengineering and Biotechnology

DOI

2020

Multiple Small RNAs Interact to Co-regulate Ethanol Tolerance in Zymomonas mobilis

Runhua Han, Katie Haning, Juan C. Gonzalez‐Rivera, Yongfu Yang, Runxia Li, Seung Hee Cho, Ju Wen Huang, Bobi A. Simonsen, Shihui Yang, Lydia M. Contreras

Frontiers in Bioengineering and Biotechnology

DOI

2020

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways

Jun Dai, Ke Li, Na Song, Wanting Yao, Huili Xia, Qiao Yang, Xiaoling Zhang, Xin Li, Zhi Wang, Lan Yao, Shihui Yang, Xiong Chen

Frontiers in Microbiology

DOI

2020

Development and characterization of acidic-pH-tolerant mutants of Zymomonas mobilis through adaptation and next-generation sequencing-based genome resequencing and RNA-Seq

Qing Yang, Yongfu Yang, Ying Tang, Xia Wang, Yunhao Chen, Wei Shen, Yangyang Zhan, Junjie Gao, Bo Wu, Mingxiong He, Shouwen Chen, Shihui Yang

Biotechnology for Biofuels

DOI

2020

Photosynthetic Conversion of Carbon Dioxide to Oleochemicals by Cyanobacteria: Recent Advances and Future Perspectives

Li Wang, Liyuan Chen, Shihui Yang, Xiaoming Tan

Frontiers in Microbiology

DOI

2020

Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production

Mengyue Qiu, Wei Shen, Xiongyin Yan, Qiaoning He, Dongbo Cai, Shouwen Chen, Hui Wei, Eric P. Knoshaug, Min Zhang, Michael E. Himmel, Shihui Yang

Biotechnology for Biofuels

DOI

2020

Transcriptomic Profiles of Zymomonas mobilis 8b to Furfural Acute and Long-Term Stress in Both Glucose and Xylose Conditions

Shihui Yang, Mary Ann Franden, Xia Wang, Yat‐Chen Chou, Yun Fu Hu, Steven D. Brown, Philip T. Pienkos, Min Zhang

Frontiers in Microbiology

DOI

2019

Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis

Wei Shen, Jun Zhang, Binan Geng, Mengyue Qiu, Mimi Hu, Qing Yang, Weiwei Bao, Yubei Xiao, Yanli Zheng, Wenfang Peng, Guimin Zhang, Lixin Ma, Shihui Yang

Microbial Cell Factories

DOI

2019

A new cold-active and alkaline pectate lyase from Antarctic bacterium with high catalytic efficiency

Yumeng Tang, Pan Wu, Sijing Jiang, Jonathan Nimal Selvaraj, Shihui Yang, Guimin Zhang

Applied Microbiology and Biotechnology

DOI

2019

CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis

Zhenghui Lu, Shihui Yang, Xin Yuan, Yunyun Shi, Li Ouyang, Sijing Jiang, Yi Li, Guimin Zhang

Nucleic Acids Research

DOI

2019

Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering

Yanli Zheng, Jiamei Han, Wenyao Liang, Runxia Li, Xiaoyun Hu, Baiyang Wang, wei shen, Lixin Ma, Lixin Ma, Li Yi, Shihui Yang, Wenfang Peng

Nucleic Acids Research

DOI

2019

Identification and characterization of ethanol‐inducible promoters of Zymomonas mobilis based on omics data and dual reporter–gene system

Yongfu Yang, Ziyue Rong, Haoyue Song, Xiuxiu Yang, Mian Li, Shihui Yang

Biotechnology and Applied Biochemistry

DOI

2019

Heterozygous diploid structure of Amorphotheca resinae ZN1 contributes efficient biodetoxification on solid pretreated corn stover

Xia Yi, Qiuqiang Gao, Lei Zhang, Xia Wang, Yanqing He, Fengxian Hu, Jian Zhang, Gen Zou, Shihui Yang, Zhihua Zhou, Jie Bao

Biotechnology for Biofuels

DOI

2019

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains

Xiaolu Li, Yu-Cai He, Libing Zhang, Zhangyang Xu, Haoxi Ben, Matthew J. Gaffrey, Yongfu Yang, Shihui Yang, Joshua S. Yuan, Weijun Qian, Bin Yang

Biotechnology for Biofuels

DOI

2019

Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era

Yongfu Yang, Wei Shen, Ju Wen Huang, Runxia Li, Yubei Xiao, Hui Wei, Yat‐Chen Chou, Min Zhang, Michael E. Himmel, Shouwen Chen, Yi Li, Lixin Ma, Shihui Yang

Biotechnology for Biofuels

DOI

2019

Engineering Zymomonas mobilis for Robust Cellulosic Ethanol Production

Juan Xia, Yongfu Yang, Chen‐Guang Liu, Shihui Yang, Feng‐Wu Bai

Trends in biotechnology

DOI

2019

Engineered Zymomonas mobilis tolerant to acetic acid and low pH via multiplex atmospheric and room temperature plasma mutagenesis

Bo Wu, Han Qin, Yiwei Yang, Guowei Duan, Shihui Yang, Fengxue Xin, Chunyan Zhao, Huanhuan Shao, Yanwei Wang, Qili Zhu, Furong Tan, Guoquan Hu, Mingxiong He

Biotechnology for Biofuels

DOI

2018

Advances and prospects in metabolic engineering of Zymomonas mobilis

Xia Wang, Qiaoning He, Yongfu Yang, Jingwen Wang, Katie Haning, Yun Fu Hu, Bo Wu, Mingxiong He, Yaoping Zhang, Jie Bao, Lydia M. Contreras, Shihui Yang

Metabolic Engineering

DOI

2018

Identification of Inhibitors in Lignocellulosic Slurries and Determination of Their Effect on Hydrocarbon-Producing Microorganisms

Shihui Yang, Mary Ann Franden, Qing Yang, Yat‐Chen Chou, Min Zhang, Philip T. Pienkos

Frontiers in Bioengineering and Biotechnology

DOI

2018

Whole genome engineering by synthesis

Zhouqing Luo, Qing Yang, Binan Geng, Shuangying Jiang, Shihui Yang, Xiaozheng Li, Yizhi Cai, Junbiao Dai

Science China Life Sciences

DOI

2018

Rewiring glycerol metabolism for enhanced production of poly-γ-glutamic acid in Bacillus licheniformis

Yangyang Zhan, Bojie Sheng, Huan Wang, Jiao Shi, Dongbo Cai, Yi Li, Shihui Yang, Zhiyou Wen, Xin Ma, Shouwen Chen

Biotechnology for Biofuels

DOI

2018

Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A

Qiaoning He, Yongfu Yang, Shihui Yang, Bryon S. Donohoe, Stefanie Van Wychen, Min Zhang, Michael E. Himmel, Eric P. Knoshaug

Biotechnology for Biofuels

DOI

2018

Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032

Shihui Yang, Jessica M. Vera, Jeff Grass, Giannis Savvakis, Oleg Vladimirovich Moskvin, Yongfu Yang, Sean J. McIlwain, Yucai Lyu, Irene Zinonos, Alexander S. Hebert, Joshua J. Coon, Donna M. Bates, Trey K. Sato, Steven D. Brown, Michael E. Himmel, Min Zhang, Robert C. Landick, Katherine M. Pappas, Yaoping Zhang

Biotechnology for Biofuels

DOI

2018

Progress and perspective on lignocellulosic hydrolysate inhibitor tolerance improvement in Zymomonas mobilis

Yongfu Yang, Mimi I-Nan Hu, Ying Tang, Binan Geng, Mengyue Qiu, Qiaoning He, Shouwen Chen, Xia Wang, Shihui Yang

Bioresources and Bioprocessing

DOI

2018

Enhanced Production of Poly-γ-glutamic acid by Overexpression of the Global Anaerobic Regulator Fnr in Bacillus licheniformis WX-02

Dongbo Cai, Shiying Hu, Yaozhong Chen, Li Liu, Shihui Yang, Xin Ma, Shouwen Chen

Applied Biochemistry and Biotechnology

DOI

2017

Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi

Qi Xu, Eric P. Knoshaug, Wei Wang, Markus Alahuhta, John O. Baker, Shihui Yang, Todd Vander Wall, Stephen R. Decker, Michael E. Himmel, Min Zhang, Hui Wei

Microbial Cell Factories

DOI

2017

Identification and Characterization of 5′ Untranslated Regions (5′UTRs) in Zymomonas mobilis as Regulatory Biological Parts

Seung Hee Cho, Katie Haning, Wei Shen, Cameron Blome, Runxia Li, Shihui Yang, Lydia M. Contreras

Frontiers in Microbiology

DOI

2017

Acetolactate synthase (AlsS) in Bacillus licheniformis WX-02: enzymatic properties and efficient functions for acetoin/butanediol and l-valine biosynthesis

Yanli Huo, Yangyang Zhan, Qin Wang, Shunyi Li, Shihui Yang, Christopher T. Nomura, Changjun Wang, Shouwen Chen

Bioprocess and Biosystems Engineering

DOI

2016

Zymomonas mobilis as a model system for production of biofuels and biochemicals

Shihui Yang, Qiang Fei, Yaoping Zhang, Lydia M. Contreras, Sagar M. Utturkar, Steven D. Brown, Michael E. Himmel, Min Zhang

Microbial Biotechnology

DOI

2016

Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars

Shihui Yang, Ali Mohagheghi, Mary Ann Franden, Yat‐Chen Chou, Xiaowen Chen, Nancy Dowe, Michael E. Himmel, Min Zhang

Biotechnology for Biofuels

DOI

2016

High-level expression and characterization of a novel serine protease in Pichia pastoris by multi-copy integration

Min Shu, Wei Shen, Shihui Yang, Xiaojuan Wang, Fei Wang, Yaping Wang, Lixin Ma

Enzyme and Microbial Technology

DOI

精选作品

期刊论文

合成生物学赋能微生物细胞疗法：智能设计与精准治疗

Synthesis, characteristics, and applications of sustainable carbon quantum dots derived from biomass of recombinant bacterium

Development of robust dominant-metabolism compromised intermediate chassis of ethanologenic Saccharomyces cerevisiae for non-ethanol biochemical production

Biological valorization of methane and nitrogen gas-derived ammonia via methanotrophic bacteria for gut-beneficial nutrients

S267P mutation of OxyR regulator in Zymomonas mobilis: mechanism of oxidative stress tolerance and applications in cellulosic hydrolysate fermentation and oxidative stress monitoring

A synergistic strategy of metabolic engineering and flocculation recycling for enhanced acetoin production in Zymomonas mobilis

Engineering ethanologenic bacterium Zymomonas mobilis for economic cellulosic bioethanol production using agro-industrial waste corncob residue

合成生物学竞赛：驱动产学研融合的新引擎

多技术融合与原料革新驱动生物制造发展

Engineering strategies and tolerance mechanisms for robust d-lactate producer development of Zymomonas mobilis

AI-powered high-throughput digital colony picker platform for sorting microbial strains by multi-modal phenotypes

Establishment of a Visible Reporter System in Zymomonas mobilis through Random Mutagenesis and Rational Design of a Chromoprotein eforRed

Establishment of a RecET-Assisted CRISPR-Cas12a System for Large Deoxyribonucleic Acid-Fragment Manipulation in Zymomonas mobilis

A dual-tag strategy with superfolder green fluorescent protein (sfGFP) and small ubiquitin-like modifier (SUMO) for soluble expression of SARS-CoV-2 RNA polymerase visibly in Escherichia coli

Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of Zymomonas mobilis for lignocellulosic ethanol production

Metabolic Engineering of Zymomonas mobilis for Xylonic Acid Production from Lignocellulosic Hydrolysate

Metabolic Engineering of Escherichia coli for Xylitol Production

Transcriptome analysis and reverse engineering verification of SNZ3Val125Ile and Pho3Asn134Asp revealed the mechanism of adaptive laboratory evolution to increase the yield of tyrosol in Saccharomyces cerevisiae strain S26-AE2

Paradigm of engineering recalcitrant non-model microorganism with dominant metabolic pathway as a biorefinery chassis

Systematic metabolic engineering of Zymomonas mobilis for β-farnesene production

Systematic investigation of TetR-family transcriptional regulators and their roles on lignocellulosic inhibitor acetate tolerance in Zymomonas mobilis

Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high d-lactate production

A novel nutritional induction strategy flexibly switching the biosynthesis of food-like products from methane by a methanotrophic bacterium

Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1 F427L mutation in the presence of glycerol

Semi-rational design and modification of phosphoketolase to improve the yield of tyrosol in Saccharomyces cerevisiae

Efficient genome-editing tools to engineer the recalcitrant non-model industrial microorganism Zymomonas mobilis

Metabolic engineering of an industrial bacterium Zymomonas mobilis for anaerobic l-serine production

Microbial Cell Factories in the Bioeconomy Era: From Discovery to Creation

生物燃料高效生产微生物细胞工厂构建研究进展

微生物硫代谢与抗逆性

Adaptive Laboratory Evolution and Metabolic Engineering of Zymomonas mobilis for Bioethanol Production Using Molasses

In vitro characterization of a pAgo nuclease TtdAgo from Thermococcus thioreducens and evaluation of its effect in vivo

Metabolic engineering of Zymomonas mobilis for co-production of D-lactic acid and ethanol using waste feedstocks of molasses and corncob residue hydrolysate

Regulation of biofilm formation in Zymomonas mobilis to enhance stress tolerance by heterologous expression of pfs and luxS

Systematic metabolic engineering of Bacillus licheniformis for hyperproduction of the antioxidant hydroxytyrosol

Isolation and Identification of Non-Saccharomyces Yeast Producing 2-Phenylethanol and Study of the Ehrlich Pathway and Shikimate Pathway

Construction and application of high-quality genome-scale metabolic model of Zymomonas mobilis to guide rational design of microbial cell factories

Improved Glutamic Acid Production Capacity of Corynebacterium glutamicum by the ARTP Mutagenesis Method

CAVE: a cloud-based platform for analysis and visualization of metabolic pathways

Metabolic Engineering of Zymomonas mobilis for Acetoin Production by Carbon Redistribution and Cofactor Balance

Synchronous efforts for burgeoning bioeconomy: The 4th international biodesign research conference and international symposium on development and application of modern biotechnology

拉曼光谱分析技术在资源微生物发酵性能评价中的应用

Synthetic refactor of essential genes decodes functionally constrained sequences in yeast genome

Evolutionary and reverse engineering in Saccharomyces cerevisiae reveals a Pdr1p mutation-dependent mechanism for 2-phenylethanol tolerance

A plasmid-free Zymomonas mobilis mutant strain reducing reactive oxygen species for efficient bioethanol production using industrial effluent of xylose mother liquor

Molecular mechanism of enhanced ethanol tolerance associated with hfq overexpression in Zymomonas mobilis

Rapamycin enhanced the production of 2-phenylethanol during whole-cell bioconversion by yeast

Determination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between Zymomonas mobilis and Escherichia coli

Transcriptomic profiling of nitrogen fixation and the role of NifA in Methylomicrobium buryatense 5GB1

Cysteine supplementation enhanced inhibitor tolerance of Zymomonas mobilis for economic lignocellulosic bioethanol production

Multilevel metabolic engineering of Bacillus licheniformis for de novo biosynthesis of 2-phenylethanol

Metabolic engineering of Zymomonas mobilis for continuous co-production of bioethanol and poly-3-hydroxybutyrate (PHB)

The potential use of Zymomonas mobilis for the food industry

Identification and Characterization of Genes Related to Ampicillin Antibiotic Resistance in Zymomonas mobilis

Benchmarking of long-read sequencing, assemblers and polishers for yeast genome

Ethanol production from lignocellulosic biomass by co-fermentation with Pecoramyces sp. F1 and Zymomonas mobilis ATCC 31821 in an integrated process

Deciphering Molecular Mechanism Underlying Self-Flocculation of Zymomonas mobilis for Robust Production

Global reprogramming of xylose metabolism in Saccharomyces cerevisiae efficiently produces ethanol from lignocellulose hydrolysates

Development of a Hyperthermostable Artificial Scaffold Based on Ultrahigh-Affinity Protein Pairs and Its Application in Cellulose Degradation

Differential analysis of ergosterol function in response to high salt and sugar stress in Zygosaccharomyces rouxii

Double nicking by RNA-directed Cascade-nCas3 for high-efficiency large-scale genome engineering

运动发酵单胞菌底盘细胞研究现状及展望

合成生物学时代基于非模式细菌的工业底盘细胞研究现状与展望

Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis

Synthetic counter-selection markers and their application in genetic modification of Synechococcus elongatus UTEX2973

Metabolic Engineering of Bacillus licheniformis for Sustainable Production of Isobutanol

Revitalizing the ethanologenic bacterium Zymomonas mobilis for sugar reduction in high-sugar-content fruits and commercial products

Development and characterization of efficient xylose utilization strains of Zymomonas mobilis

Cellulosic ethanol production by consortia of Scheffersomyces stipitis and engineered Zymomonas mobilis

A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

Year-Round Storage Operation of Three Major Agricultural Crop Residue Biomasses by Performing Dry Acid Pretreatment at Regional Collection Depots

The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance of Zymomonas mobilis

Origins and features of pectate lyases and their applications in industry

Molecular Mechanism Associated With the Impact of Methane/Oxygen Gas Supply Ratios on Cell Growth of Methylomicrobium buryatense 5GB1 Through RNA-Seq

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering

Multiple Small RNAs Interact to Co-regulate Ethanol Tolerance in Zymomonas mobilis

Zygosaccharomyces rouxii, an Aromatic Yeast Isolated From Chili Sauce, Is Able to Biosynthesize 2-Phenylethanol via the Shikimate or Ehrlich Pathways