马立新｜湖北大学｜学者主页

2026

A Programmable Eukaryotic Argonaute Nuclease with Dual DNA and RNA Cleavage Activity from Thermophilic Fungus Thermochaetoides thermophila

Zhizhao Chen, Fei Wang, Xiao-Lan Yu, Yang Liu, Lixin Ma

Small Science

DOI

2026

Structure-Based Rational Design of TcAgo from Thermogladius calderae

Xiao-Chen Xie, Wanping Chen, Shi Chen, Tianxin Cai, Chendi Zhang, Jie Chen, Zhenni Xu, Zhuang Li, Longyu Wang, Lixin Ma

Biomolecules

DOI

2026

Mechanism of the long-B prokaryotic Argonaute-mediated nuclease activation in bacterial immunity

Min Liu, Qin Yu, Xiao-Ruo Zhang, Du-Juan Shi, Qiong Xing, Wenqiang Li, Feng-Ting Huang, Lixin Ma

Nucleic Acids Research

DOI

2026

Argonaute-mediated RNA editing selectively repairs point mutations

Zhiwei Zhang, Jinyue Wang, Tongyu Guo, Xiao-Lan Yu, Fei Wang, Hang Zhang, Yang Liu, Wenqiang Li, Yi-Bin Cheng, Yan-Hong Peng, Guangbo Yan, Jiakai Cui, Lixin Ma

Nucleic Acids Research

DOI

2026

Correction: Transdermal delivery of CRISPR/Cas9-mediated melanoma gene therapy via polyamines-modified thermosensitive hydrogels

Meng Li, Songli Zhou, Suqin Zhang, Xingyu Xie, Junqi Nie, Qi Wang, Lixin Ma, Yibin Cheng, Jingwen Luo

Journal of Nanobiotechnology

DOI

2026

Author Correction: The SESAME complex regulates cell senescence through the generation of acetyl-CoA

Wanping Chen, Xilan Yu, Yinsheng Wu, Jie Tang, Qi Yu, Xiaodong Lv, Zitong Zha, 胡必成, Xin Li, Jianguo Chen, Lixin Ma, Jerry L. Workman, Shanshan Li

Nature Metabolism

DOI

2026

A Plug‐and‐Play Platform for Customizing Multivalent Degraders and Degrader‐Drug Conjugates

Mengqing Zhao, Yan Deng, Jianjian Han, Yong Xie, K. Bao, Lixin Ma, Lilong Liu, Wuxiang Mao

Advanced Science

DOI

2026

A programmable pAgo DNase from Hydrogenophilus thermoluteolus guided by small DNA and RNA

Hengrong Jiang, Fajun Lai, Yong Zhou, Jingjing Zhang, Yangyang Hu, Meng Chen, Hui Sun, Chang Guan, Sen Nie, Fei Wang, Xian Li, Wenqiang Li, Lixin Ma

International Journal of Biological Macromolecules

DOI

2026

Corrigendum to ‘Dual-layer microneedles for immunomodulation and infection-responsive therapy in diabetic foot ulcers’ [Mater. Today Bio 35 (2025) 102490]

Le Chen, Mengyi Zhan, Li Zhang, Zhuohang Xiong, Chi Zhang, Xuanxuan Zhang, Zhengyang Yang, Lixin Ma, Yibin Cheng, Huanhuan Chen

Materials Today Bio

DOI

2026

Corrigendum to ‘A one-two punch of inflammation and oxidative stress promotes revascularization for diabetic foot ulcers’ [Mater. Today Bio 31 (2025) 101548]

Li Chen, Yunrong Li, Xuanxuan Zhang, Lixin Ma, C. C. Zhang, Huanhuan Chen

Materials Today Bio

DOI

2025

基于激光时空控制表达Noxa的工程菌构建及其抗肿瘤作用

干婷芳，郑乃铭，李惠枫，徐锦瑞，吴宁宁，马立新，胡云虹

《生物工程学报》

DOI

2025

基于中空二氧化锰的多功能纳米平台增强肿瘤化学动力学治疗（英文）

葛德洁，任德宝，段亚敏，罗璇，何帅霖，钦文君，王飞，印文，马立新，杨勇，张成

Science China Materials

2025

An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum

Longyu Wang, Xiao-Chen Xie, Fu-Yong Huang, Qiang Wei, Tianxin Cai, Na Yu, Shi Chen, Fei Wang, Wanping Chen, Chin-Yu Chen, Chunhua Li, Lixin Ma

Advancement of science

DOI

2025

A Mesophilic Argonaute from Cohnella algarum Mediates Programmable DNA/RNA Cleavage with Distinctive Guide Specificity

Yan-Hong Peng, Wang Pan, Yang Wang, Yang Liu, Lixin Ma

Biomolecules

DOI

2025

Protein Engineering of Taq DNA Polymerase for Ultrafast PCR and Its Application in Molecular Diagnostics of Genetically Modified Crops.

Zhiwen Lu, Yao Yang, Yang Cheng, Binyang Deng, Wenqiang Li, Yang Liu, Fei Wang, Lixin Ma, Gang Wu, Hongfei Gao, C. Zhai

Journal of Agricultural and Food Chemistry

DOI

2025

Improving the Catalytic Activity and Thermostability of FAST-PETase with a Multifunctional Short Peptide

Jun Yang, Binyang Deng, Ping'an Liao, Siyu Lin, Li-Qin Zheng, Xing-Zhi Yang, Fei Wang, C. Zhai, Lixin Ma

Biomolecules

DOI

2025

Visual and High-Efficiency Secretion of SARS-CoV-2 Nanobodies with Escherichia coli

Shuai Zhao, Wanting Zeng, Fang Yu, Pingping Xu, Chin-Yu Chen, Wanping Chen, Yanming Dong, Fei Wang, Lixin Ma

Biomolecules

DOI

2025

Establishing a Novel E. coli Heterologous Secretion Expression System Mediated by mScarlet3 for the Expression of a Novel Lipolytic Enzyme

Jun Yang, Mingjun Yang, Hui-Chen Liu, Xin-Yu Liu, Fei Wang, Wenqiang Li, Yang Liu, C. Zhai, Lixin Ma

Biomolecules

DOI

2025

Combined Use of Engineered PfAGO and LbCas12a for Nucleic Acid Detection.

Wanting Zeng, Xiao-Chen Xie, Xiao-Lan Yu, Ting Zhang, Tianxin Cai, Shi Chen, Wanping Chen, Fei Wang, Longyu Wang, Lixin Ma

Analytical Chemistry

DOI

2025

Characterization of Argonaute Nuclease from Mesophilic Bacterium Chroococcidiopsis

Yan-Hong Peng, Yue Zhang, Yang Liu, Lixin Ma

International Journal of Molecular Sciences

DOI

2025

A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation

Jiao-Li Hu, Yangyang Hu, Yang Wang, Hengrong Jiang, M. Chen, Jingjing Zhang, Fajun Lai, Xiu-Ling Li, Yi-Bin Cheng, Tao Liu, Guoli Yang, Fei Wang, Wenqiang Li, Lixin Ma

Communications Biology

DOI

2025

In vitro programmable DNA cleavage by a eukaryotic Argonaute

Guangbo Yan, Xia Li, Longyu Wang, Fei Wang, Siyuan Lv, Xiao-Lan Yu, Zhong-Chen Li, Jiakai Cui, Baoxiang Sun, Jia Hou, Wenqiang Li, Xiao-Chen Xie, Shuai Zhao, Zhiwei Zhang, Wanping Chen, Yan-Hong Peng, Yang Liu, Lixin Ma

Nucleic Acids Research

DOI

2025

Argonaute-driven programmable multi-enzyme complex assembly on ribosomal RNA scaffolds.

Guangbo Yan, Xia Li, Xiao-Lan Yu, C. Zhai, Wenqiang Li, Lixin Ma

International Journal of Biological Macromolecules

DOI

2025

Transdermal delivery of CRISPR/Cas9-mediated melanoma gene therapy via polyamines-modified thermosensitive hydrogels

Meng Li, Songli Zhou, Suqin Zhang, Xingyu Xie, Junqi Nie, Qi Wang, Lixin Ma, Yibin Cheng, Jingwen Luo

Journal of Nanobiotechnology

DOI

2025

Somatostatin receptor-targeted polymeric nanoplatform for efficient CRISPR/Cas9 gene editing to enhance synergistic hepatocellular carcinoma therapy

Suqin Zhang, Meng Li, Jingyi Zeng, Songli Zhou, Feifan Yue, Zhaoyi Chen, Lixin Ma, Yang Wang, Fei Wang, Jingwen Luo

Journal of Nanobiotechnology

DOI

2025

A dual-tag strategy with superfolder green fluorescent protein (sfGFP) and small ubiquitin-like modifier (SUMO) for soluble expression of SARS-CoV-2 RNA polymerase visibly in Escherichia coli

Haoyue Song, Fei Wang, Wenli Xie, Zhizhao Chen, Ruyi He, Yu Fang, Qiaoning He, Shihui Yang, Lixin Ma

International Journal of Biological Macromolecules

DOI

2025

Designed peptide binders and nanobodies as PROTAC starting points for targeted degradation of PCNA and BCL6

Shuai Zhao, Jingwen Luo, Pingping Xu, Jingwei Zeng, Guangbo Yan, Fang Yu, Liwei Qin, Cheng Zhang, Peng Li, Mengxing Cai, Wuxiang Mao, Chin‐Yu Chen, Wanping Chen, Rui Han, Fei Wang, Yang Wang, Lixin Ma

International Journal of Biological Macromolecules

DOI

2025

Dual-layer microneedles for immunomodulation and infection-responsive therapy in diabetic foot ulcers

Li Chen, Mengyi Zhan, Li Zhang, Zhuohang Xiong, Chi Zhang, Xuanxuan Zhang, Zhengyang Yang, Lixin Ma, Yibin Cheng, Huanhuan Chen

Materials Today Bio

DOI

2025

A one-two punch of inflammation and oxidative stress promotes revascularization for diabetic foot ulcers

Li Chen, Yunrong Li, Xuanxuan Zhang, Lixin Ma, Cheng Zhang, Huanhuan Chen

Materials Today Bio

DOI

2024

融合大肠杆菌素DNA结合域的Taq DNA聚合酶的性质表征

王亚平，平啸寅，赵艺，刘阳，吴林，马立新

《生物工程学报》

2024

原核Argonaute的应用研究进展

王飞，李文强，刘洋，王珑瑜，陈晚苹，崔佳凯，马立新

《华中农业大学学报》

2024

Whole-Cell Display of Phospholipase D in Escherichia coli for High-Efficiency Extracellular Phosphatidylserine Production

Baoxiang Sun, Zhong-Chen Li, Yan-Hong Peng, Fei Wang, Yi-Bin Cheng, Yang Liu, Lixin Ma

Biomolecules

DOI

2024

A Fast and Sensitive One-Tube SARS-CoV-2 Detection Platform Based on RTX-PCR and Pyrococcus furiosus Argonaute

Rui Han, Fei Wang, Wanping Chen, Lixin Ma

Biosensors

DOI

2024

Structural and mechanistic insights into a mesophilic prokaryotic Argonaute

Xin Tao, Hui Ding, Shao-Wen Wu, Fei Wang, Hu Xu, Jie Li, C. Zhai, Shun-Shun Li, Kai Chen, Shan Wu, Yang Liu, Lixin Ma

Nucleic Acids Research

DOI

2024

Collateral nuclease activity of TnpB triggered by high temperature enables fast and sensitive nucleic acid detection

Ying Xu, Wen Yin, Yi-Bin Cheng, Wei Zeng, Wenqiang Li, Wanping Chen, Fei Wang, Nan Peng, Lixin Ma, Tao Liu

Communications Biology

DOI

2024

Author Correction: Collateral nuclease activity of TnpB triggered by high temperature enables fast and sensitive nucleic acid detection

Ying Xu, Wen Yin, Yi-Bin Cheng, Wei Zeng, Wenqiang Li, Wanping Chen, Fei Wang, Nan Peng, Lixin Ma, Tao Liu

Communications Biology

DOI

2024

Preamplification-free ultra-fast and ultra-sensitive point-of-care testing via LwaCas13a.

Wanting Zeng, Wanping Chen, Yang Liu, Ting Zhang, C. Zhai, Wenqiang Li, Longyu Wang, Cheng Zhang, Qili Zeng, Fei Wang, Lixin Ma

Biosensors & bioelectronics

DOI

2024

Molecular mechanism for target recognition, dimerization, and activation of Pyrococcus furiosus Argonaute.

Longyu Wang, Wanping Chen, Chendi Zhang, Xiao-Chen Xie, Fu-Yong Huang, Miaomiao Chen, Wuxiang Mao, Na Yu, Qiang Wei, Lixin Ma, Zhuang Li

Molecules and Cells

DOI

2024

Hollow MnO2-based multifunctional nanoplatform for enhanced tumor chemodynamic therapy

Dejie Ge, Debao Ren, Yamin Duan, Xuan Luo, Shasha He, Wenjun Qin, Fei Wang, Wen Yin, Lixin Ma, Yong Yang, Cheng Zhang

Science China Materials

DOI

2024

A Whole-Process Visible Strategy for the Preparation of Rhizomucor miehei Lipase with Escherichia coli Secretion Expression System and the Immobilization

Mingjun Yang, Xianhui Su, Jun Yang, Zhiwen Lu, Jie Zhou, Fei Wang, Yang Liu, Lixin Ma, Chao Zhai

Microbial Cell Factories

DOI

2024

[Characterization of a Taq DNA polymerase fused with a DNA binding domain of Escherichia coli colicin].

Yaping Wang, Xiaoyin Ping, Yi Zhao, Yang Liu, Lin Wu, Lixin Ma

PubMed

DOI

2023

Structural Basis for the Ribonuclease Activity of a Thermostable CRISPR-Cas13a from Thermoclostridium caenicola.

Feng Wang, Chendi Zhang, Haijiang Xu, Wanting Zeng, Lixin Ma, Zhuang Li

Journal of Molecular Biology

DOI

2023

Novel Nucleic Acid Detection for Human Parvovirus B19 Based on Pyrococcus furiosus Argonaute Protein

Wei-Ran Chen, Li-Yang Qiu, Ting Luo, Zhong-Qin Lu, Xueying Wang, Qiumian Hong, Jingwen Luo, Lixin Ma, Yuan Wang, Yan-Ming Dong

Viruses

DOI

2023

Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly

Xin Xiong, Zhiwen Lu, Lixin Ma, C. Zhai

Biomolecules

DOI

2023

Improving the Activity of Tryptophan Synthetase via a Nucleic Acid Scaffold

Ya-Ping Wang, Xiangyi Wang, Shuhui Niu, Wei Cheng, Xiaoyan Liu, Yong Min, Yimin Qiu, Lixin Ma, Ben Rao, Lei Zhu

Molecules

DOI

2023

A programmable pAgo nuclease with RNA target-cleavage specificity from the mesophilic bacterium Verrucomicrobia

Qi Liu, Wanping Chen, Yue Zhang, Feng Hu, Xiaoman Jiang, Fei Wang, Yang Liu, Lixin Ma

Acta Biochimica et Biophysica Sinica

DOI

2023

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

Binyang Deng, Y. Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, C. Zhai

Communications Biology

DOI

2023

In vitro characterization of a pAgo nuclease TtdAgo from Thermococcus thioreducens and evaluation of its effect in vivo

Yingke Tang, Fei Wang, Yi Wang, Yu-Wei Wang, Yang Liu, Zhizhao Chen, Wenqiang Li, Shi-Hui Yang, Lixin Ma

Frontiers in Bioengineering and Biotechnology

DOI

2023

An amplification-free CRISPR/Cas12a-based fluorescence assay for ultrasensitive detection of nuclease activity

Xinping Wang, Yi-Chuan Chen, Lixin Ma, Zhenwei Han, Yi Liu, Jie Qiao

Talanta

DOI

2023

TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method

Fang Yu, Xia Li, Fei Wang, Yang Liu, Chao Zhai, Wenqiang Li, Lixin Ma, Wanping Chen

Frontiers in Bioengineering and Biotechnology

DOI

2023

Structural Insight into the Mechanism of σ32-Mediated Transcription Initiation of Bacterial RNA Polymerase

Qiang Lu, Taiyu Chen, Jiening Wang, Feng Wang, Wenlong Ye, Lixin Ma, Shan Wu

Biomolecules

DOI

2022

Degradation of CP4-EPSPS with a Psychrophilic Bacterium Stenotrophomonas maltophilia 780

Yanhong Peng, Wen-Cong He, Yunjing Li, Lu Liu, Binyang Deng, Guangbo Yan, Jun Yang, Fei Wang, Lixin Ma, Gang Wu, C. Zhai

Biomolecules

DOI

2022

Development of a Hyperthermostable Artificial Scaffold Based on Ultrahigh-Affinity Protein Pairs and Its Application in Cellulose Degradation

Jun Yang, Fei Wang, Shi-Hui Yang, Wenxue Jiang, Rong Gao, Siyu Lin, Binyang Deng, Xiaoyu Wang, Wei Cheng, Yang Liu, Chin-Yu Chen, Qiong Xing, C. Zhai, Lixin Ma

ACS Sustainable Chemistry and Engineering

DOI

2022

Characterization of a Programmable Argonaute Nuclease from the Mesophilic Bacterium Rummeliibacillus suwonensis

Xiaoman Jiang, Yang Liu, Qi Liu, Lixin Ma

Biomolecules

DOI

2022

Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production

Shuai Zhao, Guoli Yang, Xiao-Chen Xie, Guangbo Yan, Fei Wang, Wanping Chen, Lixin Ma

Biomolecules

DOI

2022

Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation

Yanhong Peng, Ya-Ping Wang, Xiao-Yan Liu, Rong-Hua Zhou, Xian-Qing Liao, Yong Min, Lixin Ma, Ying Wang, Ben Rao

Molecules

DOI

2022

A programmable pAgo nuclease with RNA target preference from the psychrotolerant bacterium Mucilaginibacter paludis

Wenqiang Li, Yang Liu, Ruyi He, Longyu Wang, Ya-Ping Wang, Wanting Zeng, Zhiwei Zhang, Fei Wang, Lixin Ma

Nucleic Acids Research

DOI

2022

A bacterial Argonaute from Tepiditoga spiralis with the ability of RNA guided plasmid cleavage.

Xiao-Chen Xie, Longyu Wang, Lixin Ma

Biochemical and Biophysical Research Communications - BBRC

DOI

2022

A bacterial Argonaute with efficient DNA and RNA cleavage activity guided by small DNA and RNA

Longyu Wang, Xiaochen Xie, Bin Lv, Yang Liu, Wenqiang Li, Zhiwei Zhang, Jun Yang, Guangbo Yan, Wanping Chen, Cheng Zhang, Fei Wang, Chunhua Li, Lixin Ma

Cell Reports

DOI

2022

Copper‐Based Metal‐Organic Framework Induces NO Generation for Synergistic Tumor Therapy and Antimetastasis Activity

Debao Ren, Yibin Cheng, Wenxuan Xu, Wenjun Qin, Tonghui Hao, Fei Wang, Yun Fu Hu, Lixin Ma, Cheng Zhang

Small

DOI

2022

Bacteria‐Elicited Specific Thrombosis Utilizing Acid‐Induced Cytolysin A Expression to Enable Potent Tumor Therapy

Wenjun Qin, Wenxuan Xu, Longyu Wang, Debao Ren, Yibin Cheng, Wen Wu Song, Tao Jiang, Lixin Ma, Cheng Zhang

Advanced Science

DOI

2022

Encapsulating IM7-Displaying Yeast Cells in Calcium Alginate Beads for One-Step Protein Purification and Multienzyme Biocatalysis

Wenhao Yin, Xinping Wang, Ying Liao, Lixin Ma, Jie Qiao, Hui Liu, Xin Song, Yi Liu

Frontiers in Bioengineering and Biotechnology

DOI

2022

Bacteria-mediated tumor immunotherapy via photothermally-programmed PD1 expression

Wenxuan Xu, Debao Ren, Zimeng Yu, Jia Hou, Fan Huang, Tingfang Gan, Ping Ji, Cheng Zhang, Lixin Ma, Yunhong Hu

Nanoscale Advances

DOI

2022

Activation and signaling mechanism revealed by GPR119-Gs complex structures

Y. Qian, Jiening Wang, Lin-Lin Yang, Yanru Liu, Li-Na Wang, Wei Liu, Yun Lin, Hong Yang, Lixin Ma, Sheng Ye, Shan Wu, Anna Qiao

Nature Communications

DOI

2022

Phosphorylation of covalent organic framework nanospheres for inhibition of amyloid-β peptide fibrillation

Linli Yao, Zhe Zhou, Suxiao Wang, Qichao Zou, Hang‐Xing Wang, Lixin Ma, Shengfu Wang, Xun Zhang

Chemical Science

DOI

2021

基于结构改造来源于大阪伊德氏杆菌201-F6的PET水解酶

陈纯琪，韩旭，刘卫东，马立新，刘珂，郭瑞庭

《生物工程学报》

DOI

2021

CRISPR/dCas9系统在活细胞成像领域的研究进展

林斯雨，钟星，马立新，乔洁，刘奕

《生物工程学报》

DOI

2021

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

Zhiwei Zhang, Yang Liu, Jing Zhao, Wenqiang Li, Ruiwen Hu, Xia Li, Aitao Li, Ya-Ping Wang, Lixin Ma

BMC Biotechnology

DOI

2021

Corrigendum to article “A programmable omnipotent Argonaute nuclease from mesophilic bacteria Kurthia massiliensis’’

Yang Liu, Wenqiang Li, Xiaoman Jiang, Ya-Ping Wang, Zhiwei Zhang, Qi Liu, Ruyi He, Quan Chen, Jun Yang, Longyu Wang, Fei Wang, Lixin Ma

Nucleic Acids Research

DOI

2021

A programmable omnipotent Argonaute nuclease from mesophilic bacteria Kurthia massiliensis

Yang Liu, Wenqiang Li, Xiaoman Jiang, Ya-Ping Wang, Zhiwei Zhang, Qi Liu, Ruyi He, Quan Chen, Jun Yang, Longyu Wang, Fei Wang, Lixin Ma

Nucleic Acids Research

DOI

2021

General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis

Chun-Chi Chen, Xu Han, Xian Li, Pengcheng Jiang, D. Niu, Lixin Ma, Wei-Dong Liu, Siyu Li, Yingying Qu, Hebing Hu, Jian Min, Yu Yang, Lilan Zhang, Weizhen Zeng, Jian-Wen Huang, Longhai Dai, R. Guo

Nature Catalysis

DOI

2021

Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV

Longyu Wang, Ruyi He, Bin Lv, Xiao Yu, Yang Liu, Jun Yang, Wenqiang Li, Yuan Wang, Hang Zhang, Guangbo Yan, Wu-Xiang Mao, Linlin Liu, Fei Wang, Lixin Ma

Talanta: The International Journal of Pure and Applied Analytical Chemistry

DOI

2021

Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection.

Ruyi He, Longyu Wang, Fei Wang, Jun Yang, Xiao Yu, Yuan Wang, Zhiguo Liu, Chunhua Li, Lixin Ma

In Analysis

DOI

2021

High-level Extracellular Production and Immobilisation of Methyl Parathion Hydrolase from Plesiomonas sp. M6 Expressed in Pichia pastoris.

Ying Wang, Ya-Ping Wang, Can Huang, Lixin Ma, Hong Yan, Yong Min, Xiao-Yan Liu, Rao Ben

Protein Expression and Purification

DOI

2021

Cost-Effective Production of ATP and S-Adenosylmethionine Using Engineered Multidomain Scaffold Proteins

Guang-Fan Yan, Xia Li, Jun Yang, Zhong-Chen Li, Jia Hou, Ben Rao, Yong Hu, Lixin Ma, Ya-Ping Wang

Biomolecules

DOI

2021

“Aptamer-locker” DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis

Bin Qiao, Jiakun Xu, Wenhao Yin, Wanmeng Xin, Lixin Ma, Jie Qiao, Yi Liu

Biosensors and Bioelectronics

DOI

2021

Bacteria‐Mediated Tumor Therapy via Photothermally‐Programmed Cytolysin A Expression

Longyu Wang, Wenjun Qin, Wenxuan Xu, Fan Huang, Xiaochen Xie, Fei Wang, Lixin Ma, Cheng Zhang

Small

DOI

2021

A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides

Yingli Zhang, Zhongchen Li, Li Li, Ben Rao, Lixin Ma, Yaping Wang

Microorganisms

DOI

2021

Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity

Guangbo Yan, Shu Min, Shen Wei, Lixin Ma, Zhai Chao, Wang Yaping, Huang Zunxi

BMC Biotechnology

DOI

2021

The SESAME complex regulates cell senescence through the generation of acetyl-CoA

Wanping Chen, Xilan Yu, Yinsheng Wu, Jie Tang, Qi Yu, Xiaodong Lv, Zitong Zha, Bicheng Hu, Xin Li, Jianguo Chen, Lixin Ma, Jerry L. Workman, Shanshan Li

Nature Metabolism

DOI

2021

Design of fusion protein for efficient preparation of cyanovirin-n and rapid enrichment of pseudorabies virus

Bin Wang, Zhi Yang, Dan Gao, Fei Wang, Min Liu, Guanjun Chen, Lixin Ma, Xiaolan Yu

Biotechnology Letters

DOI

2021

Co-encapsulation of collagenase type I and silibinin in chondroitin sulfate coated multilayered nanoparticles for targeted treatment of liver fibrosis

Jingwen Luo, Zhiwei Zhang, Yingchun Zeng, Yanming Dong, Lixin Ma

Carbohydrate Polymers

DOI

2021

Chondroitin Sulfate-Modified Liposomes for Targeted Co-Delivery of Doxorubicin and Retinoic Acid to Suppress Breast Cancer Lung Metastasis

Zhiwei Zhang, Lixin Ma, Jingwen Luo

Pharmaceutics

DOI

2021

FlavonoidC‐Glycosyltransferases: Function, Evolutionary Relationship, Catalytic Mechanism and Protein Engineering

Longhai Dai, Yumei Hu, Chun‐Chi Chen, Lixin Ma, Rey‐Ting Guo

ChemBioEng Reviews

DOI

2021

Overview of antiviral drug candidates targeting coronaviral 3C‐like main proteases

Chun‐Chi Chen, Xuejing Yu, Chih‐Jung Kuo, Jian Min, Sizhuo Chen, Lixin Ma, Ke Liu, Rey‐Ting Guo

FEBS Journal

DOI

2021

CT5, a subtle in vitro DNA assembling method based on the combination of FnCas12a and T5 exonuclease

Mengjie Dong, Fei Wang, Bin Lv, Meng Mei, Lixin Ma, Yunhong Hu, Chao Zhai

Biotechnology Letters

DOI

2020

人细小病毒B19分子生物学研究进展

董衍明，李京京，徐鹏，李毅，马立新，王媛

《生物工程学报》

2020

Versatile cis-isoprenyl Diphosphate Synthase Superfamily Members in Catalyzing Carbon–Carbon Bond Formation

Chun-Chi Chen, Lilan Zhang, Xuejing Yu, Lixin Ma, Tzu-Ping Ko, R. Guo

DOI

2020

Terpene Cyclases and Prenyltransferases: Structures and Mechanisms of Action

Chun-Chi Chen, Satish R. Malwal, Xu Han, Wei-Dong Liu, Lixin Ma, C. Zhai, Longhai Dai, Jian-Wen Huang, A. Shillo, Janish Desai, Xianqiang Ma, Yong-Hui Zhang, R. Guo, E. Oldfield

ACS Catalysis

DOI

2020

Enzymatic degradation of plant biomass and synthetic polymers

Chun-Chi Chen, Longhai Dai, Lixin Ma, R. Guo

Nature Reviews Chemistry

DOI

2020

One-pot biocatalytic route from cycloalkanes to α,ω‐dicarboxylic acids by designed Escherichia coli consortia

Fei Wang, J. Zhao, Qian Li, Jun Yang, Ren-Jie Li, Jian Min, Xiaojuan Yu, Gao-Wei Zheng, Hui-Lei Yu, C. Zhai, C. Acevedo-Rocha, Lixin Ma, Aitao Li

Nature Communications

DOI

2020

A fusion of Taq DNA polymerase with the CL7 protein from Escherichia coli remarkably improves DNA amplification

Fei Wang, Rao Ben, Jing Ni, Lixin Ma, Yaping Wang

DOI

2020

Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase

Lilan Zhang, Zhenzhen Xie, Ziwei Liu, Shu-Yu Zhou, Lixin Ma, Wei-Dong Liu, Jian-Wen Huang, Tzu-Ping Ko, Xiuqin Li, Yuechan Hu, Jian Min, Xuejing Yu, R. Guo, Chun-Chi Chen

Nature Communications

DOI

2020

PfAgo-based detection of SARS-CoV-2

Fei Wang, Jun Yang, Ruyi He, Xiao Yu, Shu-Liang Chen, Yang Liu, Longyu Wang, Aitao Li, Linlin Liu, C. Zhai, Lixin Ma

Biosensors & bioelectronics

DOI

2020

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering

Yanli Zheng, Jie Li, Baiyang Wang, Jiamei Han, Yile Hao, Shengchen Wang, Xiangdong Ma, Shihui Yang, Lixin Ma, Yi Li, Wenfang Peng

Frontiers in Bioengineering and Biotechnology

DOI

2020

Chondroitin-modified lipid nanoparticles target the Golgi to degrade extracellular matrix for liver cancer management

Jingwen Luo, Tao Gong, Lixin Ma

Carbohydrate Polymers

DOI

2020

Structural insights into thebaine synthase 2 catalysis

Chun‐Chi Chen, Jing Xue, Wei Peng, Binju Wang, Lilan Zhang, Weidong Liu, Tzu‐Ping Ko, Jian‐Wen Huang, Shuyu Zhou, Jian Min, Lixin Ma, Longhai Dai, Rey‐Ting Guo, Xuejing Yu

Biochemical and Biophysical Research Communications

DOI

2020

Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production

Ce Dong, Jie Qiao, Xinping Wang, Wenli Sun, Lixia Chen, Shuntang Li, Ke Wu, Lixin Ma, Yi Liu

Biotechnology for Biofuels

DOI

2020

[Advances in molecular biology research on human parvovirus B19].

Yanming Dong, Jing Li, Peng Xu, Yi Li, Lixin Ma, Yuan Wang

PubMed

DOI

2020

A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris

Huahua He, Shijie Wu, Meng Mei, Jiali Ning, Chaoyin Li, Lixin Ma, Guimin Zhang, Yi Li

Frontiers in Bioengineering and Biotechnology

DOI

2020

A single digestion, single-stranded oligonucleotide mediated PCR-independent site-directed mutagenesis method

Mengjie Dong, Fei Wang, Qingqing Li, Rui Han, Aitao Li, Chao Zhai, Lixin Ma

Applied Microbiology and Biotechnology

DOI

2020

A method for the quantitative detection of Cas12a ribonucleoproteins

Jie Qiao, Siyu Lin, Wenli Sun, Lixin Ma, Yi Liu

Chemical Communications

DOI

2020

Structure basis of non-structural protein pA151R from African Swine Fever Virus

Jian‐Wen Huang, Du Niu, Ke Liu, Qian Wang, Lixin Ma, Chun‐Chi Chen, Lilan Zhang, Weidong Liu, Shuyu Zhou, Jian Min, Shan Wu, Yong Yang, Rey‐Ting Guo

Biochemical and Biophysical Research Communications

DOI

2020

SPOP negatively regulates Toll-like receptor-induced inflammation by disrupting MyD88 self-association

Yun-Hong Hu, Yang Wang, Fei Wang, Yan-Ming Dong, Wanqi Jiang, Ya-Ping Wang, X. Zhong, Lixin Ma

Cellular & Molecular Immunology

DOI

2020

Integrated Analysis of a Competing Endogenous RNA Network Reveals a Prognostic Signature in Kidney Renal Papillary Cell Carcinoma

Ruyi He, Longyu Wang, Juan Li, Lixin Ma, Fei Wang, Yang Wang

Frontiers in Cell and Developmental Biology

DOI

2020

Characterization of lncRNA-Associated ceRNA Network to Reveal Potential Prognostic Biomarkers in Lung Adenocarcinoma

Yang Wang, Ruyi He, Lixin Ma

Frontiers in Bioengineering and Biotechnology

DOI

2019

Flavin-Based Fluorescent Protein EcFbFP Auto-Guided Surface Display of Methyl Parathion Hydrolase in Escherichia coli

L. Bian, Zhen Zhang, Rongxin Tang, Wei Shen, Lixin Ma

Molecular Biotechnology

DOI

2019

Expression and Characterization of a New L-amino Acid Oxidase AAO Producing α-ketoglutaric Acid from L-glutamic Acid

Rao Ben, Xian-Qing Liao, Fang Liu, Wei Chen, Rong-Hua Zhou, Lixin Ma, Ya-Ping Wang

Biotechnology and Bioprocess Engineering

DOI

2019

Pyrococcus furiosus Argonaute-mediated nucleic acid detection.

Ruyi He, Longyu Wang, Fei Wang, Wenqiang Li, Yang Liu, Aitao Li, Yuan Wang, Wu-Xiang Mao, C. Zhai, Lixin Ma

Chemical Communications

DOI

2019

Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering

Yanli Zheng, Jiamei Han, Wenyao Liang, Runxia Li, Xiaoyun Hu, Baiyang Wang, wei shen, Lixin Ma, Lixin Ma, Li Yi, Shihui Yang, Wenfang Peng

Nucleic Acids Research

DOI

2019

Establishment and application of a CRISPR–Cas12a assisted genome-editing system in Zymomonas mobilis

Wei Shen, Jun Zhang, Binan Geng, Mengyue Qiu, Mimi Hu, Qing Yang, Weiwei Bao, Yubei Xiao, Yanli Zheng, Wenfang Peng, Guimin Zhang, Lixin Ma, Shihui Yang

Microbial Cell Factories

DOI

2019

Author Correction: Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins

Jie Qiao, Wenqiang Li, Siyu Lin, Wenli Sun, Lixin Ma, Yi Liu

Communications Biology

DOI

2019

Soluble expression of single-chain variable fragment (scFv) in Escherichia coli using superfolder green fluorescent protein as fusion partner

Min Liu, Bin Wang, Fei Wang, Zhi Yang, Dan Gao, Chenyao Zhang, Lixin Ma, Xiaolan Yu

Applied Microbiology and Biotechnology

DOI

2019

Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1

Jiantao Ma, Tzu‐Ping Ko, Xuejing Yu, Lilan Zhang, Lixin Ma, Chao Zhai, Rey‐Ting Guo, Weidong Liu, Huazhong Li, Chun‐Chi Chen

Biochemical and Biophysical Research Communications

DOI

2019

Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins

Jie Qiao, Wenqiang Li, Siyu Lin, Wenli Sun, Lixin Ma, Yi Liu

Communications Biology

DOI

2019

A Highly Efficient Indirect P. pastoris Surface Display Method Based on the CL7/Im7 Ultra-High-Affinity System

Shuntang Li, Jie Qiao, Siyu Lin, Yi Liu, Lixin Ma

Molecules

DOI

2019

Prediction and characterization of promoters and ribosomal binding sites of Zymomonas mobilis in system biology era

Yongfu Yang, Wei Shen, Ju Wen Huang, Runxia Li, Yubei Xiao, Hui Wei, Yat‐Chen Chou, Min Zhang, Michael E. Himmel, Shouwen Chen, Yi Li, Lixin Ma, Shihui Yang

Biotechnology for Biofuels

DOI

2019

One-step production of bioactive human lipopolysaccharide binding protein from LPS-eliminated E. coli

Jie Qiao, Ce Dong, Xinping Wang, Yi Liu, Lixin Ma

Protein Expression and Purification

DOI

2019

A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease

Hu Xu, Qian Wang, Zhiwei Zhang, Yi Li, Lixin Ma, Chao Zhai

Enzyme and Microbial Technology

DOI

2019

Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis

Chih‐Jung Kuo, Jian Gao, Jian‐Wen Huang, Tzu‐Ping Ko, Chao Zhai, Lixin Ma, Weidong Liu, Longhai Dai, Yung‐Fu Chang, Ter-Hsin Chen, Yumei Hu, Xuejing Yu, Rey‐Ting Guo, Chun‐Chi Chen

Biochimica et Biophysica Acta (BBA) - General Subjects

DOI

2019

Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology

Hexiao Shen, Li Li, Zhaowei Teng, Tianqing Meng, Xiangbin Kong, Yan Ping Hu, Yun Kai Zhu, Lixin Ma

Scientific Reports

DOI

2018

全细胞生物催化邻氯扁桃腈不对称合成(R)-邻氯扁桃酸

陈晨，韩瑞，佘文文，倪静，马立新

《湖北大学学报(自然科学版)》

2018

Rapid and Error-Free Site-Directed Mutagenesis by a PCR-Free In Vitro CRISPR/Cas9-Mediated Mutagenic System.

Wen-Wen She, Jing Ni, Ke Shui, Fei Wang, Ruyi He, Jinhui Xue, M. Reetz, Aitao Li, Lixin Ma

ACS Synthetic Biology

DOI

2018

Back Cover: Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase (Angew. Chem. Int. Ed. 3/2019)

Hangyu Zhou, Binju Wang, Fei Wang, Xiaojuan Yu, Lixin Ma, Aitao Li, Manfred T. Reetz

Angewandte Chemie International Edition

DOI

2018

Rücktitelbild: Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase (Angew. Chem. 3/2019)

Hangyu Zhou, Binju Wang, Fei Wang, Xiaojuan Yu, Lixin Ma, Aitao Li, Manfred T. Reetz

Angewandte Chemie

DOI

2018

Functional expression of porcine interferon-α using a combinational strategy in Pichia pastoris GS115

Huahua He, Chao Zhai, Meng Mei, Yi Rao, Yao Liu, Fei Wang, Lixin Ma, Zhengbing Jiang, Guimin Zhang, Yi Li

Enzyme and Microbial Technology

DOI

2018

Chemo‐ and Regioselective Dihydroxylation of Benzene to Hydroquinone Enabled by Engineered Cytochrome P450 Monooxygenase

Hangyu Zhou, Binju Wang, Fei Wang, Xiaojuan Yu, Lixin Ma, Aitao Li, Manfred T. Reetz

Angewandte Chemie

DOI

2017

High-level expression of l-glutamate oxidase in Pichia pastoris using multi-copy expression strains and high cell density cultivation.

Ya-Ping Wang, Rao Ben, Hong Yan, Rui Han, Li Li, Ping'an Liao, Lixin Ma

Protein Expression and Purification

DOI

2017

Regulation of DNA replication-coupled histone gene expression

Qianyun Mei, Junhua Huang, Wanping Chen, Jie Tang, Chen Xu, Qi Yu, Ying Cheng, Lixin Ma, Xilan Yu, Shanshan Li

Oncotarget

DOI

2017

In vivo Assembly in Escherichia coli of Transformation Vectors for Plastid Genome Engineering

Yuyong Wu, Lili You, Shengchun Li, Meiqi Ma, Mengting Wu, Lixin Ma, Ralph Bock, Ling Chang, Jiang Zhang

Frontiers in Plant Science

DOI

2017

High copy and stable expression of the xylanase XynHB in Saccharomyces cerevisiae by rDNA-mediated integration

Cheng Fang, Qinhong Wang, Jonathan Nimal Selvaraj, Yuling Zhou, Lixin Ma, Guimin Zhang, Yanhe Ma

Scientific Reports

DOI

2017

Non-peptide guided auto-secretion of recombinant proteins by super-folder green fluorescent protein in Escherichia coli

Zhen Zhang, Rongxing Tang, Dewu Zhu, Wenfeng Wang, Yi Li, Lixin Ma

Scientific Reports

DOI

2017

High-level expression and characterization of solvent-tolerant lipase

Yu Wang, Dan Luo, Yunshi Zhao, Shufang Tian, Wenpeng Deng, Chunhua Li, Lixin Ma

Journal of Bioscience and Bioengineering

DOI

2017

Linear DNA vaccine prepared by large-scale PCR provides protective immunity against H1N1 influenza virus infection in mice

Fei Wang, Quanjiao Chen, Shuntang Li, Chenyao Zhang, Shanshan Li, Min Liu, Kun Mei, Chunhua Li, Lixin Ma, Xiaolan Yu

Veterinary Microbiology

DOI

2017

High-level expression of a bacterial laccase, CueO from Escherichia coli K12 in Pichia pastoris GS115 and its application on the decolorization of synthetic dyes

Xiaojian Ma, Lu Liu, Qingqing Li, Yunyun Liu, Yi Li, Lixin Ma, Chao Zhai

Enzyme and Microbial Technology

DOI

2017

Regulation of SESAME-mediated H3T11 phosphorylation by glycolytic enzymes and metabolites

Qi Yu, Chong Tong, Mingdan Luo, Xiangyan Xue, Qianyun Mei, Lixin Ma, Xiaolan Yu, Xiaolan Yu, Wuxiang Mao, Lingbao Kong, Xilan Yu, Xilan Yu, Shanshan Li

PLoS ONE

DOI

2017

High-level expression of two thermophilic β-mannanases in Yarrowialipolytica

Wang YaPing, Ben Rao, Ling Zhang, Lixin Ma

Protein Expression and Purification

DOI

2017

Characterization of aromatic residue–controlled protein retention in the endoplasmic reticulum of Saccharomyces cerevisiae

Meng Mei, Chao Zhai, Xinzhi Li, Yu Zhou, Wenfang Peng, Lixin Ma, Qinhong Wang, Brent L. Iverson, Guimin Zhang, Yi Li

Journal of Biological Chemistry

DOI

2017

Kinetic basis for the activation of human cyclooxygenase-2 rather than cyclooxygenase-1 by nitric oxide

Jie Qiao, Lixin Ma, Justine P. Roth, Yamin Li, Yi Liu

Organic & Biomolecular Chemistry

DOI

2016

Dithiolopyrrolones的合成、广谱生物学活性及其应用（英文）

蔡浩宜，赵新露，梅萌，周瑜，张琳玲，沈文文，马立新，何结望，易犁

《中国抗生素杂志》

2016

Application of modified yeast surface display technologies for non-Antibody protein engineering

Meng Mei, Yu Zhou, Wenfang Peng, Chan Yu, Lixin Ma, Guimin Zhang, Yi Li

Microbiological Research

DOI

2016

Surface Immobilization of Human Arginase-1 with an Engineered Ice Nucleation Protein Display System in E. coli

Zhen Zhang, Rongxin Tang, Lu Bian, Meng Mei, Chunhua Li, Xiangdong Ma, Yi Li, Lixin Ma

PLoS ONE

DOI

2016

High-level expression and characterization of a novel serine protease in Pichia pastoris by multi-copy integration

Min Shu, Wei Shen, Shihui Yang, Xiaojuan Wang, Fei Wang, Yaping Wang, Lixin Ma

Enzyme and Microbial Technology

DOI

2016

Dynamic diversification of bacterial functional groups in the Baiyunbian liquor stacking fermentation process

Hui Li, Ruyi He, Xiaomao Xiong, Mingchun Zhang, Tuanyuan Yang, Zhengbing Jiang, Lixin Ma, Yi Li, Xiangdong Ma

Annals of Microbiology

DOI

2016

The Functions of Effector Proteins in Yersinia Virulence

Linglin Zhang, Meng Mei, Chan Yu, WENWEN SHEN, Lixin Ma, Jiewang He, Yi Li

Polish Journal of Microbiology

DOI

2016

Trbp Is Required for Differentiation of Myoblasts and Normal Regeneration of Skeletal Muscle

Jian Ding, Mao Nie, Jianming Liu, Xiaoyun Hu, Lixin Ma, Zhong‐Liang Deng, Da‐Zhi Wang

PLoS ONE

DOI

2015

油菜叶绿体乙酰辅酶A羧化酶单交换表达载体的构建

武玉永，谭秀华，马立新

《广西植物》

2015

High level expression of organophosphorus hydrolase in Pichia pastoris by multicopy ophcM assembly

Wei Shen, Min Shu, Lixin Ma, Hong Ni, Hong Yan

Protein Expression and Purification

DOI

2015

Secretory expression of organophosphorus hydrolase OPHC2 inYarrowia lipolyticaPolg

Meng Li, Xiaolan Yu, Fei Wang, Chao Zhai, Wei Shen, Xianhong Yu, Xiaojuan Wang, Lixin Ma

Journal of Environmental Science and Health Part B

DOI

2015

Expression and Characterization of the RKOD DNA Polymerase in Pichia pastoris

Fei Wang, Shuntang Li, Hui Zhao, Lu Bian, Liang Chen, Zhen Zhang, Xing Zhong, Lixin Ma, Xiaolan Yu

PLoS ONE

DOI

2015

High-level expression and characterization of a thermophilic β-mannanase from Aspergillus niger in Pichia pastoris

Yu Shi, Zhezhe Li, Yaping Wang, Wanping Chen, Lin Fu, Wei Tang, Chen Chen, Yunyun Liu, Xue Zhang, Lixin Ma

Biotechnology Letters

DOI

2015

Trbp regulates heart function through microRNA-mediated Sox6 repression

Jian Ding, Jinghai Chen, Yanqun Wang, Masaharu Kataoka, Lixin Ma, Pingzhu Zhou, Xiaoyun Hu, Zhiqiang Lin, Mao Nie, Zhong-Liang Deng, William T. Pu, Da-Zhi Wang

Nature Genetics

DOI

2014

耐热羧肽酶Taq基因在毕赤酵母中的表达

余先红，汪晓娟，钟星，唐微，翟超，陈晚苹，马立新

《生物工程学报》

2014

Novel characteristics of chitin deacetylase from Colletotrichum lindemuthianum: Production of fully acetylated chitooligomers, and hydrolysis of deacetylated chitooligomers

Lixin Kang, Yunxiang Liang, Lixin Ma

Process Biochemistry

DOI

2014

Expression of recombinant proteins in insect cells by their direct infection with Escherichia coli transformed with baculovirus bacmids

Xinyi Yu, Jonggang Hua, G. HU, J. ZHANG, Lixin Ma

Acta Virologica

DOI

2013

构建定向T载体用于基因克隆和表达

钟星，翟超，陈亮，余晓岚，蒋思婧，严红，杨登想，马立新

《生物工程学报》

2013

油菜叶绿体多顺反子双交换表达载体的构建(英文)

武玉永，姚庆收，马立新

Agricultural Science & Technology

2013

Four novel antimicrobial peptides derived from human C8α-MACPF

Zhen Zhang, Yuling Zhou, Jing Ya Sun, Peihong Mao, Jing Xiang, Xiangdong Ma, Lixin Ma

Biotechnology Letters

DOI

2013

A single-step mixing cloning method for assembly of lentiviral short hairpin RNA expression vectors for gene silencing

Xing Zhong, Chao Zhai, Yang Deng-xiang, Sijing Jiang, Zhezhe Li, Xiaolan Yu, Liang Chen, Zhen Zhang, Fei Wang, Yapin Wang, Wan‐Ping Chen, Lixin Ma

Analytical Biochemistry

DOI

2013

High-Level Expression and Characteristics of A Thermostable Phytase Mutant from Escherichia Coli K12 in Pichia Pastoris

Lixin Ma

Chemistry & Bioengineering

2013

The sumoylation of zinc-fingers and homeoboxes 1 (ZHX1) by ubc9 regulates its stability and transcriptional repression activity

Shuliang Chen, Yu Xiao, Quan Lei, Lixin Ma, Deyin Guo

Journal of Cellular Biochemistry

DOI

2012

壳聚糖酶的克隆表达与壳寡糖的制备分析

康立新，周玉玲，马立新

《生物技术》

2012

Recombinant expression of chitosanase from Bacillus subtilis HD145 in Pichia pastoris

Lixin Kang, Xiao-Mei Chen, Ling Fu, Lixin Ma

Carbohydrate Research

DOI

2012

Cloning and Expression of Chitosanase and Preparation and Analysis of Chitooligosaccharide

Lixin Ma

Biotechnology(Faisalabad)

2012

Cloning of Novel Mannanase Gene and Its Secreted Expression in Pichia Pastoris

Lixin Ma

Pharmaceutical biotechnology

2012

Screening and application of a high efficient feather-degrading probiotics

Lixin Ma

Journal of Hubei University

2011

High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris

Xiaomei Chen, Chao Zhai, Lixin Kang, Chunhua Li, Hong Yan, Yuling Zhou, Xiaolan Yu, Lixin Ma

Biotechnology Letters

DOI

2011

Molecular cloning and heterologous expression of an acid stable xylanase gene from Alternaria sp. HB186

Liangwei Mao, Po Meng, Cheng Zhou, Lixin Ma, Guimin Zhang, Yanhe Ma

World Journal of Microbiology and Biotechnology

DOI

2011

An improved nonchromatographic method for the purification of recombinant proteins using elastin-like polypeptide-tagged proteases

Dongming Lan, Guangrui Huang, Hongwei Shao, Lichun Zhang, Lixin Ma, Shangwu Chen, Anlong Xu

Analytical Biochemistry

DOI

2011

Improved method for constructing plant amiRNA vectors with blue–white screening and MAGIC

Hong Yan, Xing Zhong, Sijing Jiang, Chao Zhai, Lixin Ma

Biotechnology Letters

DOI

2011

Comparative studies on the enzymology properties of severval phytases expressed by Pichia pastoris

Lixin Ma

Journal of Hubei University

2011

Toward an understanding of the protein interaction network of the human liver

Jian Wang, Keke Huo, Lixin Ma, Liujun Tang, Dong Li, Xiaobi Huang, Yanzhi Yuan, Chunhua Li, Wei Wang, Wei Guan, Hui Chen, Chaozhi Jin, Junchen Wei, Wanqiao Zhang, Yongsheng Yang, Qiongming Liu, Ying Zhou, Cuili Zhang, Zhihao Wu, Wang‐Xiang Xu, Ying Zhang, Tao Liu, Donghui Yu, Ya‐Ping Zhang, Liang Chen, Dewu Zhu, Xing Zhong, Lixin Kang, Xiang Gan, Xiaolan Yu, Qi Ma, Jing Ru Yan, Li Zhou, Zhongyang Liu, Yunping Zhu, Tao Zhou, Fuchu He, Xiaoming Yang

Molecular Systems Biology

DOI

2010

植酸酶phyA基因在解脂耶氏酵母po1h中的表达

陈云，邹由，王一丁，马立新

《生物工程学报》

2010

A novel approach for the construction of plant amiRNA expression vectors

Hong Yan, Xuan Deng, Yinglong Cao, Junyan Huang, Lixin Ma, Bin Zhao

Journal of Biotechnology

DOI

2010

Constructing recombinant herpesvirus BAC vectors with mating-assisted genetically integrated clone method

Sijing Jiang, Xing Zhong, Chao Zhai, Liang Chen, Lixin Ma, Meilin Jin, Huanchun Chen

Biotechnology Letters

DOI

2010

Efficient vectors for expression cloning of large numbers of PCR fragments in P. pastoris

Ben Rao, Xing Zhong, Yaping Wang, Qiong Wu, Zhengbing Jiang, Lixin Ma

Yeast

DOI

2010

Preparation of Monoclonal Antibodies Against KOD DNA Polymerase

Lixin Ma

Chemistry & Bioengineering

2009

红霉素高产菌株的筛选

李武德，唐慧慧，万丹，马立新，宋喜芳

《中国抗生素杂志》

2009

利用融合蛋白EDDIE在大肠杆菌中高效表达抗菌肽Cecropin AD

张贞，柯涛，周玉玲，马向东，马立新

《生物工程学报》

2009

A series of novel directional cloning and expression vectors for blunt-end ligation of PCR products

Qiong Wu, Xing Zhong, Chao Zhai, Ju Yang, Xiaomei Chen, Liang Chen, Wenfeng Wang, Lixin Ma

Biotechnology Letters

DOI

2009

An advanced blue–white screening method for construction of shRNA expression vectors

Ju Yang, Jun Qiang Xu, Xing Zhong, Yun Zhang, Qiong Wu, Wenjuan Li, Xing Zhang, Peng Zhen, Xiaosong Yang, Liang Chen, Yaping Wang, Dewu Zhu, Chao Zhai, Lixin Ma

Biochemical and Biophysical Research Communications

DOI

2009

Novel ligation-independent cloning vectors for production of native proteins

Lixin Ma

Journal of Hubei University

2009

High-throughput cloning of human liver complete open reading frames using homologous recombination in Escherichia coli

Dewu Zhu, Xin Zhong, Rong Tan, Liang Chen, Guangrui Huang, Jing Li, Xiaoyan Sun, Li‐an Xu, Jin Chen, Yangping Ou, Ting Zhang, Dongxia Yuan, Zhen Zhang, Wangyun Shu, Lixin Ma

Analytical Biochemistry

DOI

2008

棉花黄萎病真菌Verticillium dahliae木聚糖酶基因的克隆、表达和酶学性质分析

张桂敏，饶犇，叶戋，马立新，张先恩

《微生物学报》

2008

甘蓝型油菜叶绿体多顺反子单交换表达载体构建

武玉永，姚庆收，马立新

《安徽农业科学》

2008

Cloning and enzymatic characterization of a xylanase gene from a soil-derived metagenomic library with an efficient approach

Yong Hu, Guimin Zhang, Aiying Li, Jing Chen, Lixin Ma

Applied Microbiology and Biotechnology

DOI

2008

A rapid and reliable method for screening recombinants in E.coli

Lixin Ma

Journal of Hubei University

2008

Subunit–subunit interactions in the human 26S proteasome

Chuan Chen, Caoxin Huang, Shuiliang Chen, Jie Liang, Wenbo Lin, Guifen Ke, Hongxin Zhang, Bing Wang, Jian Huang, Ze‐Guang Han, Lixin Ma, Keke Huo, Xiaoming Yang, Pengyuan Yang, Fuchu He, Tao Tao

PROTEOMICS

DOI

2007

一种鉴定多糖水解酶类及其产生菌的新方法

马向东，柯涛，熊兰，严红，马立新

《微生物学报》

2007

植酸酶和甘露聚糖酶双功能毕赤酵母工程菌的构建和产酶分析

黄生平，汪昌丽，张桂敏，马立新

《微生物学报》

2007

一种新型巴斯德毕赤酵母表达载体的构建及甘露聚糖酶的表达

黄生平，汪昌丽，马立新

《生物技术通讯》

2007

Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli

Jing Li, Chunhua Li, Wei Xiao, Dongxia Yuan, Gang Wan, Lixin Ma

Analytical Biochemistry

DOI

2007

The mutagenic properties of BrdUTP in a random mutagenesis process

Xiangdong Ma, Tao Ke, Peihong Mao, Xiang Jin, Lixin Ma, Guangyuan He

Molecular Biology Reports

DOI

2007

High expression of codon optimized phytase-encoding gene appA-P in Pichia Pastoris

Lixin Ma

Journal of Hubei University

2007

Cloning of a Laccase Gene cDNA from Fungi Ganoderma lucidum by the Method of Exon Spicing

Lixin Ma

Journal of Wuhan University

2007

Cell surface display of functionally active lipases from Yarrowia lipolytica in Pichia pastoris

Zhengbing Jiang, Huiting Song, Nishith Gupta, Lixin Ma, Zhenbin Wu

Protein Expression and Purification

DOI

2006

番茄质体多顺反子定点整合表达载体的构建及其转烟草的研究

陆云华，马立新，蒋思婧

《作物学报》

2006

Directional and Direct Cloning Strategy for High-Throughput Generation of Recombinant Baculoviruses

Qi Ma, Li Zhou, Lixin Ma, Keke Huo

BioTechniques

DOI

2006

Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216

Huiting Song, Zhengbing Jiang, Lixin Ma

Protein Expression and Purification

DOI

2006

Molecular cloning and heterologous expression of an alkaline xylanase fromBacillus pumilusHBP8 inPichia pastoris

Guimin Zhang, Yong Hu, Zhuang Yong-hong, Lixin Ma, Xian‐En Zhang

Biocatalysis and Biotransformation

DOI

2006

Cloning of glyA Gene and Its Expression in Pichia pastoris

Lixin Ma

Amino Acids & Biotic Resources

2006

An efficient protocol for extraction of fungal chromosomal DNA

Lixin Ma

Journal of Hubei University

2006

一种构建基因组文库的改进方法

徐松，张娟，马立新

《遗传》

2006

一种通用高效的复杂载体构建的新方法

陆云华，马立新，蒋思婧

《遗传》

2005

水稻多顺反子质体表达载体的构建

陆云华，马立新

《宜春学院学报》

2005

烟草质体多顺反子定点整合表达载体的构建和转化

陆云华，马立新，陈俊，蒋思婧

《植物生理与分子生物学》

2005

安全环保的烟草质体多顺反子定点整合表达载体的构建

陆云华，张新，马立新

《宜春学院学报》

2005

平菇漆酶基因在毕赤酵母中的分泌表达及酶学性质研究

张银波，江木兰，胡小加，张桂敏，马立新

《微生物学报》

2005

耐碱性甘露聚糖酶基因的克隆及其在毕赤酵母中的表达

谭秀华，武玉永，马立新，蒋思婧

《微生物学报》

2005

灵芝(Ganoderma lucidum)漆酶基因的克隆及其序列分析

张银波，江木兰，胡小加，张桂敏，马立新

《中国生物化学与分子生物学报》

2005

大豆叶绿体多顺反子单交换表达载体的构建

常玲，张江，陈俊，张银波，马立新

《中国油料作物学报》

2005

Cloning of β-1,4endo-glucanase gene and expression in Escherichia coli

Lixin Ma

Journal of Hubei University

2005

The expression of oligo-1,6-glucosidase from Bacillus subtilis in Pichia pastoris

Lixin Ma

Journal of Hubei University

2005

酸性木聚糖酶基因的克隆及其在毕赤酵母中的分泌表达

李春华，李翔，马立新

《微生物学通报》

2005

结核杆菌Ag85B与ESAT-6双顺反子真核表达质粒的构建及体外表达

刘焰，刘君炎，马立新，居巍，彭剑虹，刘汉燕

《免疫学杂志》

2004

植酸酶的基因工程与应用

张江，马立新

《现代商贸工业》

2004

金针菇漆酶基因的克隆及其在毕赤酵母中的表达研究

张银波，姜琼，江木兰，马立新

《微生物学报》

2004

Cloning of phytase gene from Bacillus licheniformis and its expression in Escherichia coli

Lixin Ma

Journal of Hubei University

2003

Arresten在毕赤酵母中的表达和鉴定

曾昭淳，何爱彬，马立新，廖飞

《生物工程学报》

2003

短小芽孢杆菌木聚糖酶基因在毕赤酵母中的分泌表达及酶学性质研究

江正兵，宋慧婷，马立新

《生物工程学报》

2002

枯草芽孢杆菌寡聚-1,6-葡萄糖苷酶基因的克隆及其在大肠杆菌中的表达

蒋思婧，马立新

《微生物学报》

1999

适用于细菌与植物互作研究的gusA启动子探针载体pHN112的构建

史巧娟，马立新，蒋思婧，周俊初

《生物工程学报》

1999

以绿荧光蛋白基因为报告基因的广宿主启动子探针载体的构建和应用

马立新，史巧娟，周俊初，陈华癸

《微生物学报》

精选作品

期刊论文

A Programmable Eukaryotic Argonaute Nuclease with Dual DNA and RNA Cleavage Activity from Thermophilic Fungus Thermochaetoides thermophila

Structure-Based Rational Design of TcAgo from Thermogladius calderae

Mechanism of the long-B prokaryotic Argonaute-mediated nuclease activation in bacterial immunity

Argonaute-mediated RNA editing selectively repairs point mutations

Correction: Transdermal delivery of CRISPR/Cas9-mediated melanoma gene therapy via polyamines-modified thermosensitive hydrogels

Author Correction: The SESAME complex regulates cell senescence through the generation of acetyl-CoA

A Plug‐and‐Play Platform for Customizing Multivalent Degraders and Degrader‐Drug Conjugates

A programmable pAgo DNase from Hydrogenophilus thermoluteolus guided by small DNA and RNA

Corrigendum to ‘Dual-layer microneedles for immunomodulation and infection-responsive therapy in diabetic foot ulcers’ [Mater. Today Bio 35 (2025) 102490]

Corrigendum to ‘A one-two punch of inflammation and oxidative stress promotes revascularization for diabetic foot ulcers’ [Mater. Today Bio 31 (2025) 101548]

基于激光时空控制表达Noxa的工程菌构建及其抗肿瘤作用

基于中空二氧化锰的多功能纳米平台增强肿瘤化学动力学治疗（英文）

An Engineered PfAgo with Wide Catalytic Temperature Range and Substrate Spectrum

A Mesophilic Argonaute from Cohnella algarum Mediates Programmable DNA/RNA Cleavage with Distinctive Guide Specificity

Protein Engineering of Taq DNA Polymerase for Ultrafast PCR and Its Application in Molecular Diagnostics of Genetically Modified Crops.

Improving the Catalytic Activity and Thermostability of FAST-PETase with a Multifunctional Short Peptide

Visual and High-Efficiency Secretion of SARS-CoV-2 Nanobodies with Escherichia coli

Establishing a Novel E. coli Heterologous Secretion Expression System Mediated by mScarlet3 for the Expression of a Novel Lipolytic Enzyme

Combined Use of Engineered PfAGO and LbCas12a for Nucleic Acid Detection.

Characterization of Argonaute Nuclease from Mesophilic Bacterium Chroococcidiopsis

A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation

In vitro programmable DNA cleavage by a eukaryotic Argonaute

Argonaute-driven programmable multi-enzyme complex assembly on ribosomal RNA scaffolds.

Transdermal delivery of CRISPR/Cas9-mediated melanoma gene therapy via polyamines-modified thermosensitive hydrogels

Somatostatin receptor-targeted polymeric nanoplatform for efficient CRISPR/Cas9 gene editing to enhance synergistic hepatocellular carcinoma therapy

A dual-tag strategy with superfolder green fluorescent protein (sfGFP) and small ubiquitin-like modifier (SUMO) for soluble expression of SARS-CoV-2 RNA polymerase visibly in Escherichia coli

Designed peptide binders and nanobodies as PROTAC starting points for targeted degradation of PCNA and BCL6

Dual-layer microneedles for immunomodulation and infection-responsive therapy in diabetic foot ulcers

A one-two punch of inflammation and oxidative stress promotes revascularization for diabetic foot ulcers

融合大肠杆菌素DNA结合域的Taq DNA聚合酶的性质表征

原核Argonaute的应用研究进展

Whole-Cell Display of Phospholipase D in Escherichia coli for High-Efficiency Extracellular Phosphatidylserine Production

A Fast and Sensitive One-Tube SARS-CoV-2 Detection Platform Based on RTX-PCR and Pyrococcus furiosus Argonaute

Structural and mechanistic insights into a mesophilic prokaryotic Argonaute

Collateral nuclease activity of TnpB triggered by high temperature enables fast and sensitive nucleic acid detection

Author Correction: Collateral nuclease activity of TnpB triggered by high temperature enables fast and sensitive nucleic acid detection

Preamplification-free ultra-fast and ultra-sensitive point-of-care testing via LwaCas13a.

Molecular mechanism for target recognition, dimerization, and activation of Pyrococcus furiosus Argonaute.

Hollow MnO2-based multifunctional nanoplatform for enhanced tumor chemodynamic therapy

A Whole-Process Visible Strategy for the Preparation of Rhizomucor miehei Lipase with Escherichia coli Secretion Expression System and the Immobilization

[Characterization of a Taq DNA polymerase fused with a DNA binding domain of Escherichia coli colicin].

Structural Basis for the Ribonuclease Activity of a Thermostable CRISPR-Cas13a from Thermoclostridium caenicola.

Novel Nucleic Acid Detection for Human Parvovirus B19 Based on Pyrococcus furiosus Argonaute Protein

Applications of Programmable Endonucleases in Sequence- and Ligation-Independent Seamless DNA Assembly

Improving the Activity of Tryptophan Synthetase via a Nucleic Acid Scaffold

A programmable pAgo nuclease with RNA target-cleavage specificity from the mesophilic bacterium Verrucomicrobia

Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification

In vitro characterization of a pAgo nuclease TtdAgo from Thermococcus thioreducens and evaluation of its effect in vivo

An amplification-free CRISPR/Cas12a-based fluorescence assay for ultrasensitive detection of nuclease activity

TLTC, a T5 exonuclease–mediated low-temperature DNA cloning method

Structural Insight into the Mechanism of σ32-Mediated Transcription Initiation of Bacterial RNA Polymerase

Degradation of CP4-EPSPS with a Psychrophilic Bacterium Stenotrophomonas maltophilia 780

Development of a Hyperthermostable Artificial Scaffold Based on Ultrahigh-Affinity Protein Pairs and Its Application in Cellulose Degradation

Characterization of a Programmable Argonaute Nuclease from the Mesophilic Bacterium Rummeliibacillus suwonensis

Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production

Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation

A programmable pAgo nuclease with RNA target preference from the psychrotolerant bacterium Mucilaginibacter paludis

A bacterial Argonaute from Tepiditoga spiralis with the ability of RNA guided plasmid cleavage.

A bacterial Argonaute with efficient DNA and RNA cleavage activity guided by small DNA and RNA

Copper‐Based Metal‐Organic Framework Induces NO Generation for Synergistic Tumor Therapy and Antimetastasis Activity

Bacteria‐Elicited Specific Thrombosis Utilizing Acid‐Induced Cytolysin A Expression to Enable Potent Tumor Therapy

Encapsulating IM7-Displaying Yeast Cells in Calcium Alginate Beads for One-Step Protein Purification and Multienzyme Biocatalysis

Bacteria-mediated tumor immunotherapy via photothermally-programmed PD1 expression

Activation and signaling mechanism revealed by GPR119-Gs complex structures

Phosphorylation of covalent organic framework nanospheres for inhibition of amyloid-β peptide fibrillation

基于结构改造来源于大阪伊德氏杆菌201-F6的PET水解酶

CRISPR/dCas9系统在活细胞成像领域的研究进展

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid

Corrigendum to article “A programmable omnipotent Argonaute nuclease from mesophilic bacteria Kurthia massiliensis’’

A programmable omnipotent Argonaute nuclease from mesophilic bacteria Kurthia massiliensis

General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis

Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV

Combination of ultrashort PCR and Pyrococcus furiosus Argonaute for DNA detection.

High-level Extracellular Production and Immobilisation of Methyl Parathion Hydrolase from Plesiomonas sp. M6 Expressed in Pichia pastoris.

Cost-Effective Production of ATP and S-Adenosylmethionine Using Engineered Multidomain Scaffold Proteins

“Aptamer-locker” DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis

Bacteria‐Mediated Tumor Therapy via Photothermally‐Programmed Cytolysin A Expression

A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides